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Transcriptome Analysis Revealed Plant Hormone Biosynthesis and Response Pathway Modification by Epichloë gansuensis in Achnatherum inebrians under Different Soil Moisture Availability

The present study was designed to explore the effects of the endophyte Epichloë gansuensis on gene expression related to plant hormone biosynthesis and response pathways and the content of salicylic acid (SA) and jasmonic acid (JA) hormones of Achnatherum inebrians, under different moisture conditio...

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Autores principales: Zhao, Zhenrui, Kou, Mingzhu, Zhong, Rui, Xia, Chao, Christensen, Michael J., Zhang, Xingxu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398561/
https://www.ncbi.nlm.nih.gov/pubmed/34436179
http://dx.doi.org/10.3390/jof7080640
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author Zhao, Zhenrui
Kou, Mingzhu
Zhong, Rui
Xia, Chao
Christensen, Michael J.
Zhang, Xingxu
author_facet Zhao, Zhenrui
Kou, Mingzhu
Zhong, Rui
Xia, Chao
Christensen, Michael J.
Zhang, Xingxu
author_sort Zhao, Zhenrui
collection PubMed
description The present study was designed to explore the effects of the endophyte Epichloë gansuensis on gene expression related to plant hormone biosynthesis and response pathways and the content of salicylic acid (SA) and jasmonic acid (JA) hormones of Achnatherum inebrians, under different moisture conditions. Through a pot experiment and transcriptome analysis, we found a total of 51 differentially expressed genes (DEGs) related to hormone biosynthesis and response pathways, including 12 auxin related genes, 8 cytokinin (CTK) related genes, 3 gibberellin (GA) related genes, 7 abscisic acid (ABA) related genes, 7 ethylene (ET) related genes, 12 JA related genes and 4 SA related genes. Furthermore, key genes of JA and SA biosynthesis and response pathways, such as LOX2S, AOS, OPR, ACX, JMT, JAZ, PAL, NPR1, TGA and PR-1, showed different degrees of upregulation or downregulation. Under 60% soil moisture content, the JA content of endophyte-free (EF) A. inebrians was significantly (p < 0.05) higher than that of endophyte-infected (EI) A. inebrians. Under 30% and 60% soil moisture content, the SA content of EF A. inebrians was significantly (p < 0.05) higher than that of EI A. inebrians. SA content of EI A. inebrians under 30% and 60% soil moisture content was significantly (p < 0.05) higher than that under 15% soil moisture content. With both EI and EF plants, the SA and JA levels, respectively, are very similar at 15% soil moisture content. This study has revealed that E. gansuensis differentially activated plant hormone synthesis and signal transduction pathways of A. inebrians plants under different soil moisture availability.
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spelling pubmed-83985612021-08-29 Transcriptome Analysis Revealed Plant Hormone Biosynthesis and Response Pathway Modification by Epichloë gansuensis in Achnatherum inebrians under Different Soil Moisture Availability Zhao, Zhenrui Kou, Mingzhu Zhong, Rui Xia, Chao Christensen, Michael J. Zhang, Xingxu J Fungi (Basel) Article The present study was designed to explore the effects of the endophyte Epichloë gansuensis on gene expression related to plant hormone biosynthesis and response pathways and the content of salicylic acid (SA) and jasmonic acid (JA) hormones of Achnatherum inebrians, under different moisture conditions. Through a pot experiment and transcriptome analysis, we found a total of 51 differentially expressed genes (DEGs) related to hormone biosynthesis and response pathways, including 12 auxin related genes, 8 cytokinin (CTK) related genes, 3 gibberellin (GA) related genes, 7 abscisic acid (ABA) related genes, 7 ethylene (ET) related genes, 12 JA related genes and 4 SA related genes. Furthermore, key genes of JA and SA biosynthesis and response pathways, such as LOX2S, AOS, OPR, ACX, JMT, JAZ, PAL, NPR1, TGA and PR-1, showed different degrees of upregulation or downregulation. Under 60% soil moisture content, the JA content of endophyte-free (EF) A. inebrians was significantly (p < 0.05) higher than that of endophyte-infected (EI) A. inebrians. Under 30% and 60% soil moisture content, the SA content of EF A. inebrians was significantly (p < 0.05) higher than that of EI A. inebrians. SA content of EI A. inebrians under 30% and 60% soil moisture content was significantly (p < 0.05) higher than that under 15% soil moisture content. With both EI and EF plants, the SA and JA levels, respectively, are very similar at 15% soil moisture content. This study has revealed that E. gansuensis differentially activated plant hormone synthesis and signal transduction pathways of A. inebrians plants under different soil moisture availability. MDPI 2021-08-06 /pmc/articles/PMC8398561/ /pubmed/34436179 http://dx.doi.org/10.3390/jof7080640 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Zhenrui
Kou, Mingzhu
Zhong, Rui
Xia, Chao
Christensen, Michael J.
Zhang, Xingxu
Transcriptome Analysis Revealed Plant Hormone Biosynthesis and Response Pathway Modification by Epichloë gansuensis in Achnatherum inebrians under Different Soil Moisture Availability
title Transcriptome Analysis Revealed Plant Hormone Biosynthesis and Response Pathway Modification by Epichloë gansuensis in Achnatherum inebrians under Different Soil Moisture Availability
title_full Transcriptome Analysis Revealed Plant Hormone Biosynthesis and Response Pathway Modification by Epichloë gansuensis in Achnatherum inebrians under Different Soil Moisture Availability
title_fullStr Transcriptome Analysis Revealed Plant Hormone Biosynthesis and Response Pathway Modification by Epichloë gansuensis in Achnatherum inebrians under Different Soil Moisture Availability
title_full_unstemmed Transcriptome Analysis Revealed Plant Hormone Biosynthesis and Response Pathway Modification by Epichloë gansuensis in Achnatherum inebrians under Different Soil Moisture Availability
title_short Transcriptome Analysis Revealed Plant Hormone Biosynthesis and Response Pathway Modification by Epichloë gansuensis in Achnatherum inebrians under Different Soil Moisture Availability
title_sort transcriptome analysis revealed plant hormone biosynthesis and response pathway modification by epichloë gansuensis in achnatherum inebrians under different soil moisture availability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398561/
https://www.ncbi.nlm.nih.gov/pubmed/34436179
http://dx.doi.org/10.3390/jof7080640
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