Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression

The upregulated expression of tyrosine kinase AXL has been reported in several hematologic and solid human tumors, including gastric, breast, colorectal, prostate and ovarian cancers. Thus, AXL can potentially serve as a diagnostic and prognostic biomarker for various cancers. This paper reports the...

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Autores principales: Sreejith, Kamalalayam Rajan, Umer, Muhammad, Singha, Pradip, Nguyen, Nhat-Khuong, Kasetsirikul, Surasak, Ooi, Chin Hong, Shiddiky, Muhammad J. A., Nguyen, Nam-Trung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398586/
https://www.ncbi.nlm.nih.gov/pubmed/34442532
http://dx.doi.org/10.3390/mi12080905
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author Sreejith, Kamalalayam Rajan
Umer, Muhammad
Singha, Pradip
Nguyen, Nhat-Khuong
Kasetsirikul, Surasak
Ooi, Chin Hong
Shiddiky, Muhammad J. A.
Nguyen, Nam-Trung
author_facet Sreejith, Kamalalayam Rajan
Umer, Muhammad
Singha, Pradip
Nguyen, Nhat-Khuong
Kasetsirikul, Surasak
Ooi, Chin Hong
Shiddiky, Muhammad J. A.
Nguyen, Nam-Trung
author_sort Sreejith, Kamalalayam Rajan
collection PubMed
description The upregulated expression of tyrosine kinase AXL has been reported in several hematologic and solid human tumors, including gastric, breast, colorectal, prostate and ovarian cancers. Thus, AXL can potentially serve as a diagnostic and prognostic biomarker for various cancers. This paper reports the first ever loop-mediated isothermal amplification (LAMP) in a core-shell bead assay for the detection of AXL gene overexpression. We demonstrated simple instrumentation toward a point-of-care device to perform LAMP. This paper also reports the first ever use of core-shell beads as a microreactor to perform LAMP as an attempt to promote environmentally-friendly laboratory practices.
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spelling pubmed-83985862021-08-29 Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression Sreejith, Kamalalayam Rajan Umer, Muhammad Singha, Pradip Nguyen, Nhat-Khuong Kasetsirikul, Surasak Ooi, Chin Hong Shiddiky, Muhammad J. A. Nguyen, Nam-Trung Micromachines (Basel) Article The upregulated expression of tyrosine kinase AXL has been reported in several hematologic and solid human tumors, including gastric, breast, colorectal, prostate and ovarian cancers. Thus, AXL can potentially serve as a diagnostic and prognostic biomarker for various cancers. This paper reports the first ever loop-mediated isothermal amplification (LAMP) in a core-shell bead assay for the detection of AXL gene overexpression. We demonstrated simple instrumentation toward a point-of-care device to perform LAMP. This paper also reports the first ever use of core-shell beads as a microreactor to perform LAMP as an attempt to promote environmentally-friendly laboratory practices. MDPI 2021-07-30 /pmc/articles/PMC8398586/ /pubmed/34442532 http://dx.doi.org/10.3390/mi12080905 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sreejith, Kamalalayam Rajan
Umer, Muhammad
Singha, Pradip
Nguyen, Nhat-Khuong
Kasetsirikul, Surasak
Ooi, Chin Hong
Shiddiky, Muhammad J. A.
Nguyen, Nam-Trung
Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression
title Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression
title_full Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression
title_fullStr Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression
title_full_unstemmed Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression
title_short Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression
title_sort loop-mediated isothermal amplification in a core-shell bead assay for the detection of tyrosine kinase axl overexpression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398586/
https://www.ncbi.nlm.nih.gov/pubmed/34442532
http://dx.doi.org/10.3390/mi12080905
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