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The Dynamics of Flower Development in Castanea sativa Mill.

The sweet chestnut tree (Castanea sativa Mill.) is one of the most significant Mediterranean tree species, being an important natural resource for the wood and fruit industries. It is a monoecious species, presenting unisexual male catkins and bisexual catkins, with the latter having distinct male a...

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Autores principales: Alhinho, Ana Teresa, Ramos, Miguel Jesus Nunes, Alves, Sofia, Rocheta, Margarida, Morais-Cecílio, Leonor, Gomes-Laranjo, José, Sobral, Rómulo, Costa, Maria Manuela Ribeiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398726/
https://www.ncbi.nlm.nih.gov/pubmed/34451583
http://dx.doi.org/10.3390/plants10081538
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author Alhinho, Ana Teresa
Ramos, Miguel Jesus Nunes
Alves, Sofia
Rocheta, Margarida
Morais-Cecílio, Leonor
Gomes-Laranjo, José
Sobral, Rómulo
Costa, Maria Manuela Ribeiro
author_facet Alhinho, Ana Teresa
Ramos, Miguel Jesus Nunes
Alves, Sofia
Rocheta, Margarida
Morais-Cecílio, Leonor
Gomes-Laranjo, José
Sobral, Rómulo
Costa, Maria Manuela Ribeiro
author_sort Alhinho, Ana Teresa
collection PubMed
description The sweet chestnut tree (Castanea sativa Mill.) is one of the most significant Mediterranean tree species, being an important natural resource for the wood and fruit industries. It is a monoecious species, presenting unisexual male catkins and bisexual catkins, with the latter having distinct male and female flowers. Despite the importance of the sweet chestnut tree, little is known regarding the molecular mechanisms involved in the determination of sexual organ identity. Thus, the study of how the different flowers of C. sativa develop is fundamental to understand the reproductive success of this species and the impact of flower phenology on its productivity. In this study, a C. sativa de novo transcriptome was assembled and the homologous genes to those of the ABCDE model for floral organ identity were identified. Expression analysis showed that the C. sativa B- and C-class genes are differentially expressed in the male flowers and female flowers. Yeast two-hybrid analysis also suggested that changes in the canonical ABCDE protein–protein interactions may underlie the mechanisms necessary to the development of separate male and female flowers, as reported for the monoecious Fagaceae Quercus suber. The results here depicted constitute a step towards the understanding of the molecular mechanisms involved in unisexual flower development in C. sativa, also suggesting that the ABCDE model for flower organ identity may be molecularly conserved in the predominantly monoecious Fagaceae family.
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spelling pubmed-83987262021-08-29 The Dynamics of Flower Development in Castanea sativa Mill. Alhinho, Ana Teresa Ramos, Miguel Jesus Nunes Alves, Sofia Rocheta, Margarida Morais-Cecílio, Leonor Gomes-Laranjo, José Sobral, Rómulo Costa, Maria Manuela Ribeiro Plants (Basel) Article The sweet chestnut tree (Castanea sativa Mill.) is one of the most significant Mediterranean tree species, being an important natural resource for the wood and fruit industries. It is a monoecious species, presenting unisexual male catkins and bisexual catkins, with the latter having distinct male and female flowers. Despite the importance of the sweet chestnut tree, little is known regarding the molecular mechanisms involved in the determination of sexual organ identity. Thus, the study of how the different flowers of C. sativa develop is fundamental to understand the reproductive success of this species and the impact of flower phenology on its productivity. In this study, a C. sativa de novo transcriptome was assembled and the homologous genes to those of the ABCDE model for floral organ identity were identified. Expression analysis showed that the C. sativa B- and C-class genes are differentially expressed in the male flowers and female flowers. Yeast two-hybrid analysis also suggested that changes in the canonical ABCDE protein–protein interactions may underlie the mechanisms necessary to the development of separate male and female flowers, as reported for the monoecious Fagaceae Quercus suber. The results here depicted constitute a step towards the understanding of the molecular mechanisms involved in unisexual flower development in C. sativa, also suggesting that the ABCDE model for flower organ identity may be molecularly conserved in the predominantly monoecious Fagaceae family. MDPI 2021-07-27 /pmc/articles/PMC8398726/ /pubmed/34451583 http://dx.doi.org/10.3390/plants10081538 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alhinho, Ana Teresa
Ramos, Miguel Jesus Nunes
Alves, Sofia
Rocheta, Margarida
Morais-Cecílio, Leonor
Gomes-Laranjo, José
Sobral, Rómulo
Costa, Maria Manuela Ribeiro
The Dynamics of Flower Development in Castanea sativa Mill.
title The Dynamics of Flower Development in Castanea sativa Mill.
title_full The Dynamics of Flower Development in Castanea sativa Mill.
title_fullStr The Dynamics of Flower Development in Castanea sativa Mill.
title_full_unstemmed The Dynamics of Flower Development in Castanea sativa Mill.
title_short The Dynamics of Flower Development in Castanea sativa Mill.
title_sort dynamics of flower development in castanea sativa mill.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398726/
https://www.ncbi.nlm.nih.gov/pubmed/34451583
http://dx.doi.org/10.3390/plants10081538
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