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A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers. The DNA-core histone complexes were formed using λ-DNA tethers, core histones, and NAP1 and were exposed to forces ranging from ~2 pN to ~...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398817/ https://www.ncbi.nlm.nih.gov/pubmed/34443369 http://dx.doi.org/10.3390/molecules26164781 |
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author | Fabian, Roberto Gaire, Santosh Tyson, Christopher Adhikari, Raghabendra Pegg, Ian Sarkar, Abhijit |
author_facet | Fabian, Roberto Gaire, Santosh Tyson, Christopher Adhikari, Raghabendra Pegg, Ian Sarkar, Abhijit |
author_sort | Fabian, Roberto |
collection | PubMed |
description | We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers. The DNA-core histone complexes were formed using λ-DNA tethers, core histones, and NAP1 and were exposed to forces ranging from ~2 pN to ~74 pN. During the assembly events, we observed the length of the DNA decrease in approximate integer multiples of ~50 nm, suggesting the binding of the histone octamers to the DNA tether. During the mechanically induced disassembly events, we observed disruption lengths in approximate integer multiples of ~50 nm, suggesting the unbinding of one or more octamers from the DNA tether. We also observed histone octamer unbinding events at forces as low as ~2 pN. Our horizontal magnetic tweezers yielded high-resolution, low-noise data on force-mediated DNA-core histone assembly and disassembly processes. |
format | Online Article Text |
id | pubmed-8398817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83988172021-08-29 A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins Fabian, Roberto Gaire, Santosh Tyson, Christopher Adhikari, Raghabendra Pegg, Ian Sarkar, Abhijit Molecules Article We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers. The DNA-core histone complexes were formed using λ-DNA tethers, core histones, and NAP1 and were exposed to forces ranging from ~2 pN to ~74 pN. During the assembly events, we observed the length of the DNA decrease in approximate integer multiples of ~50 nm, suggesting the binding of the histone octamers to the DNA tether. During the mechanically induced disassembly events, we observed disruption lengths in approximate integer multiples of ~50 nm, suggesting the unbinding of one or more octamers from the DNA tether. We also observed histone octamer unbinding events at forces as low as ~2 pN. Our horizontal magnetic tweezers yielded high-resolution, low-noise data on force-mediated DNA-core histone assembly and disassembly processes. MDPI 2021-08-07 /pmc/articles/PMC8398817/ /pubmed/34443369 http://dx.doi.org/10.3390/molecules26164781 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fabian, Roberto Gaire, Santosh Tyson, Christopher Adhikari, Raghabendra Pegg, Ian Sarkar, Abhijit A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins |
title | A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins |
title_full | A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins |
title_fullStr | A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins |
title_full_unstemmed | A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins |
title_short | A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins |
title_sort | horizontal magnetic tweezers for studying single dna molecules and dna-binding proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398817/ https://www.ncbi.nlm.nih.gov/pubmed/34443369 http://dx.doi.org/10.3390/molecules26164781 |
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