Study on the Regulatory Effects of GA(3) on Soybean Internode Elongation

Excessive plant height is an important factor that can lead to lodging, which is closely related to soybean yield. Gibberellins are widely used as plant growth regulators in agricultural production. Gibberellic acid (GA(3)), one of the most effective active gibberellins, has been used to regulate plant height and increase yields. The mechanism through which GA(3) regulates internode elongation has been extensively investigated. In 2019 and 2020, we applied GA(3) to the stems, leaves, and roots of two soybean cultivars, Heinong 48 (a high-stalk cultivar) and Henong 60 (a dwarf cultivar), and GA(3) was also applied to plants whose apical meristem was removed or to girded plants to compare the internode length and stem GA(3) content of soybean plants under different treatments. These results suggested that the application of GA(3) to the stems, leaves, and roots of soybean increased the internode length and GA(3) content in the stems. Application of GA(3) decreased the proportion of the pith in the soybean stems and primary xylem while increasing the proportion of secondary xylem. The apical meristem is an important site of GA(3) synthesis in soybean stems and is involved in the regulation of stem elongation. GA(3) was shown to be transported acropetally through the xylem and laterally between the xylem and phloem in soybean stems. We conclude that the GA(3) level in stems is an important factor affecting internode elongation.