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Laser Scribing Fabrication of Graphitic Carbon Biosensors for Label-Free Detection of Interleukin-6
Interleukin-6 (IL-6) is an important immuno-modulating cytokine playing a pivotal role in inflammatory processes in disease induction and progression. As IL-6 serves as an important indicator of disease state, it is of paramount importance to develop low cost, fast and sensitive improved methods of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399033/ https://www.ncbi.nlm.nih.gov/pubmed/34443939 http://dx.doi.org/10.3390/nano11082110 |
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author | Tan, Pei Shee Vaughan, Eoghan Islam, Jahidul Burke, Niall Iacopino, Daniela Tierney, Joanna B. |
author_facet | Tan, Pei Shee Vaughan, Eoghan Islam, Jahidul Burke, Niall Iacopino, Daniela Tierney, Joanna B. |
author_sort | Tan, Pei Shee |
collection | PubMed |
description | Interleukin-6 (IL-6) is an important immuno-modulating cytokine playing a pivotal role in inflammatory processes in disease induction and progression. As IL-6 serves as an important indicator of disease state, it is of paramount importance to develop low cost, fast and sensitive improved methods of detection. Here we present an electrochemical immunosensor platform based on the use of highly porous graphitic carbon electrodes fabricated by direct laser writing of commercial polyimide tapes and chemically modified with capture IL-6 antibodies. The unique porous and 3D morphology, as well as the high density of edge planes of the graphitic carbon electrodes, resulted in a fast heterogeneous electron transfer (HET) rate, k(0) = 0.13 cm/s. The resulting immunosensor showed a linear response to log of concentration in the working range of 10 to 500 pg/mL, and low limit of detection (LOD) of 5.1 pg/mL IL-6 in phosphate buffer saline. The total test time was approximately 90 min, faster than the time required for ELISA testing. Moreover, the assay did not require additional sample pre-concentration or labelling steps. The immunosensor shelf-life was long, with stable results obtained after 6 weeks of storage at 4 °C, and the selectivity was high, as no response was obtained in the presence of another inflammatory cytokine, Interlukin-4. These results show that laser-fabricated graphitic carbon electrodes can be used as selective and sensitive electrochemical immunosensors and offer a viable option for rapid and low-cost biomarker detection for point-of-care analysis. |
format | Online Article Text |
id | pubmed-8399033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83990332021-08-29 Laser Scribing Fabrication of Graphitic Carbon Biosensors for Label-Free Detection of Interleukin-6 Tan, Pei Shee Vaughan, Eoghan Islam, Jahidul Burke, Niall Iacopino, Daniela Tierney, Joanna B. Nanomaterials (Basel) Article Interleukin-6 (IL-6) is an important immuno-modulating cytokine playing a pivotal role in inflammatory processes in disease induction and progression. As IL-6 serves as an important indicator of disease state, it is of paramount importance to develop low cost, fast and sensitive improved methods of detection. Here we present an electrochemical immunosensor platform based on the use of highly porous graphitic carbon electrodes fabricated by direct laser writing of commercial polyimide tapes and chemically modified with capture IL-6 antibodies. The unique porous and 3D morphology, as well as the high density of edge planes of the graphitic carbon electrodes, resulted in a fast heterogeneous electron transfer (HET) rate, k(0) = 0.13 cm/s. The resulting immunosensor showed a linear response to log of concentration in the working range of 10 to 500 pg/mL, and low limit of detection (LOD) of 5.1 pg/mL IL-6 in phosphate buffer saline. The total test time was approximately 90 min, faster than the time required for ELISA testing. Moreover, the assay did not require additional sample pre-concentration or labelling steps. The immunosensor shelf-life was long, with stable results obtained after 6 weeks of storage at 4 °C, and the selectivity was high, as no response was obtained in the presence of another inflammatory cytokine, Interlukin-4. These results show that laser-fabricated graphitic carbon electrodes can be used as selective and sensitive electrochemical immunosensors and offer a viable option for rapid and low-cost biomarker detection for point-of-care analysis. MDPI 2021-08-19 /pmc/articles/PMC8399033/ /pubmed/34443939 http://dx.doi.org/10.3390/nano11082110 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tan, Pei Shee Vaughan, Eoghan Islam, Jahidul Burke, Niall Iacopino, Daniela Tierney, Joanna B. Laser Scribing Fabrication of Graphitic Carbon Biosensors for Label-Free Detection of Interleukin-6 |
title | Laser Scribing Fabrication of Graphitic Carbon Biosensors for Label-Free Detection of Interleukin-6 |
title_full | Laser Scribing Fabrication of Graphitic Carbon Biosensors for Label-Free Detection of Interleukin-6 |
title_fullStr | Laser Scribing Fabrication of Graphitic Carbon Biosensors for Label-Free Detection of Interleukin-6 |
title_full_unstemmed | Laser Scribing Fabrication of Graphitic Carbon Biosensors for Label-Free Detection of Interleukin-6 |
title_short | Laser Scribing Fabrication of Graphitic Carbon Biosensors for Label-Free Detection of Interleukin-6 |
title_sort | laser scribing fabrication of graphitic carbon biosensors for label-free detection of interleukin-6 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399033/ https://www.ncbi.nlm.nih.gov/pubmed/34443939 http://dx.doi.org/10.3390/nano11082110 |
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