Cargando…

Optimization of Production Parameters for Andrographolide-Loaded Nanoemulsion Preparation by Microfluidization and Evaluations of Its Bioactivities in Skin Cancer Cells and UVB Radiation-Exposed Skin

Andrographolide (AG) is an active compound isolated from Andrographis paniculata (Family Acanthaceae). Although it possesses beneficial bioactivities to the skin, there is insufficient information of its applications for treatment of skin disorders due to low water solubility leading to complication...

Descripción completa

Detalles Bibliográficos
Autores principales: Asasutjarit, Rathapon, Sooksai, Nawarat, Fristiohady, Adryan, Lairungruang, Kriyapa, Ng, Shiow-Fern, Fuongfuchat, Asira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399116/
https://www.ncbi.nlm.nih.gov/pubmed/34452250
http://dx.doi.org/10.3390/pharmaceutics13081290
_version_ 1783744998876905472
author Asasutjarit, Rathapon
Sooksai, Nawarat
Fristiohady, Adryan
Lairungruang, Kriyapa
Ng, Shiow-Fern
Fuongfuchat, Asira
author_facet Asasutjarit, Rathapon
Sooksai, Nawarat
Fristiohady, Adryan
Lairungruang, Kriyapa
Ng, Shiow-Fern
Fuongfuchat, Asira
author_sort Asasutjarit, Rathapon
collection PubMed
description Andrographolide (AG) is an active compound isolated from Andrographis paniculata (Family Acanthaceae). Although it possesses beneficial bioactivities to the skin, there is insufficient information of its applications for treatment of skin disorders due to low water solubility leading to complications in product development. To overcome the problem, an AG-loaded nanoemulsion (AG-NE) was formulated and prepared using a microfluidization technique. This study aimed to investigate the effect of pressure and the number of homogenization cycles (factors) on droplet size, polydispersity index and zeta potential of AG-NE (responses) and to determine the effect of AG-NE on skin cancer cells and UVB irradiation-induced skin disorders in rats. Relationships between factors versus responses obtained from the face-centered central composite design were described by quadratic models. The optimum value of parameters for the production of optimized AG-NE (Op-AG-NE) were 20,000 psi of pressure and 5 homogenization cycles. Op-AG-NE showed promising cytotoxicity effects on the human malignant melanoma- (A375 cells) and non-melanoma cells (A-431 cells) via apoptosis induction with a high selectivity index and also inhibited intracellular tyrosinase activity in the A375 cells. Op-AG-NE could reduce melanin index and healed UVB irradiation exposed skin. Op-AG-NE thus had potential for treatment of skin cancers and skin disorders from exposure to UVB radiation.
format Online
Article
Text
id pubmed-8399116
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83991162021-08-29 Optimization of Production Parameters for Andrographolide-Loaded Nanoemulsion Preparation by Microfluidization and Evaluations of Its Bioactivities in Skin Cancer Cells and UVB Radiation-Exposed Skin Asasutjarit, Rathapon Sooksai, Nawarat Fristiohady, Adryan Lairungruang, Kriyapa Ng, Shiow-Fern Fuongfuchat, Asira Pharmaceutics Article Andrographolide (AG) is an active compound isolated from Andrographis paniculata (Family Acanthaceae). Although it possesses beneficial bioactivities to the skin, there is insufficient information of its applications for treatment of skin disorders due to low water solubility leading to complications in product development. To overcome the problem, an AG-loaded nanoemulsion (AG-NE) was formulated and prepared using a microfluidization technique. This study aimed to investigate the effect of pressure and the number of homogenization cycles (factors) on droplet size, polydispersity index and zeta potential of AG-NE (responses) and to determine the effect of AG-NE on skin cancer cells and UVB irradiation-induced skin disorders in rats. Relationships between factors versus responses obtained from the face-centered central composite design were described by quadratic models. The optimum value of parameters for the production of optimized AG-NE (Op-AG-NE) were 20,000 psi of pressure and 5 homogenization cycles. Op-AG-NE showed promising cytotoxicity effects on the human malignant melanoma- (A375 cells) and non-melanoma cells (A-431 cells) via apoptosis induction with a high selectivity index and also inhibited intracellular tyrosinase activity in the A375 cells. Op-AG-NE could reduce melanin index and healed UVB irradiation exposed skin. Op-AG-NE thus had potential for treatment of skin cancers and skin disorders from exposure to UVB radiation. MDPI 2021-08-18 /pmc/articles/PMC8399116/ /pubmed/34452250 http://dx.doi.org/10.3390/pharmaceutics13081290 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Asasutjarit, Rathapon
Sooksai, Nawarat
Fristiohady, Adryan
Lairungruang, Kriyapa
Ng, Shiow-Fern
Fuongfuchat, Asira
Optimization of Production Parameters for Andrographolide-Loaded Nanoemulsion Preparation by Microfluidization and Evaluations of Its Bioactivities in Skin Cancer Cells and UVB Radiation-Exposed Skin
title Optimization of Production Parameters for Andrographolide-Loaded Nanoemulsion Preparation by Microfluidization and Evaluations of Its Bioactivities in Skin Cancer Cells and UVB Radiation-Exposed Skin
title_full Optimization of Production Parameters for Andrographolide-Loaded Nanoemulsion Preparation by Microfluidization and Evaluations of Its Bioactivities in Skin Cancer Cells and UVB Radiation-Exposed Skin
title_fullStr Optimization of Production Parameters for Andrographolide-Loaded Nanoemulsion Preparation by Microfluidization and Evaluations of Its Bioactivities in Skin Cancer Cells and UVB Radiation-Exposed Skin
title_full_unstemmed Optimization of Production Parameters for Andrographolide-Loaded Nanoemulsion Preparation by Microfluidization and Evaluations of Its Bioactivities in Skin Cancer Cells and UVB Radiation-Exposed Skin
title_short Optimization of Production Parameters for Andrographolide-Loaded Nanoemulsion Preparation by Microfluidization and Evaluations of Its Bioactivities in Skin Cancer Cells and UVB Radiation-Exposed Skin
title_sort optimization of production parameters for andrographolide-loaded nanoemulsion preparation by microfluidization and evaluations of its bioactivities in skin cancer cells and uvb radiation-exposed skin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399116/
https://www.ncbi.nlm.nih.gov/pubmed/34452250
http://dx.doi.org/10.3390/pharmaceutics13081290
work_keys_str_mv AT asasutjaritrathapon optimizationofproductionparametersforandrographolideloadednanoemulsionpreparationbymicrofluidizationandevaluationsofitsbioactivitiesinskincancercellsanduvbradiationexposedskin
AT sooksainawarat optimizationofproductionparametersforandrographolideloadednanoemulsionpreparationbymicrofluidizationandevaluationsofitsbioactivitiesinskincancercellsanduvbradiationexposedskin
AT fristiohadyadryan optimizationofproductionparametersforandrographolideloadednanoemulsionpreparationbymicrofluidizationandevaluationsofitsbioactivitiesinskincancercellsanduvbradiationexposedskin
AT lairungruangkriyapa optimizationofproductionparametersforandrographolideloadednanoemulsionpreparationbymicrofluidizationandevaluationsofitsbioactivitiesinskincancercellsanduvbradiationexposedskin
AT ngshiowfern optimizationofproductionparametersforandrographolideloadednanoemulsionpreparationbymicrofluidizationandevaluationsofitsbioactivitiesinskincancercellsanduvbradiationexposedskin
AT fuongfuchatasira optimizationofproductionparametersforandrographolideloadednanoemulsionpreparationbymicrofluidizationandevaluationsofitsbioactivitiesinskincancercellsanduvbradiationexposedskin