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Genome-Wide Expression Patterns of Rhoptry Kinases during the Eimeria tenella Life-Cycle

Kinome from apicomplexan parasites is composed of eukaryotic protein kinases and Apicomplexa specific kinases, such as rhoptry kinases (ROPK). Ropk is a gene family that is known to play important roles in host–pathogen interaction in Toxoplasma gondii but is still poorly described in Eimeria tenell...

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Autores principales: Ribeiro E Silva, Adeline, Sausset, Alix, Bussière, Françoise I., Laurent, Fabrice, Lacroix-Lamandé, Sonia, Silvestre, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399136/
https://www.ncbi.nlm.nih.gov/pubmed/34442701
http://dx.doi.org/10.3390/microorganisms9081621
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author Ribeiro E Silva, Adeline
Sausset, Alix
Bussière, Françoise I.
Laurent, Fabrice
Lacroix-Lamandé, Sonia
Silvestre, Anne
author_facet Ribeiro E Silva, Adeline
Sausset, Alix
Bussière, Françoise I.
Laurent, Fabrice
Lacroix-Lamandé, Sonia
Silvestre, Anne
author_sort Ribeiro E Silva, Adeline
collection PubMed
description Kinome from apicomplexan parasites is composed of eukaryotic protein kinases and Apicomplexa specific kinases, such as rhoptry kinases (ROPK). Ropk is a gene family that is known to play important roles in host–pathogen interaction in Toxoplasma gondii but is still poorly described in Eimeria tenella, the parasite responsible for avian coccidiosis worldwide. In the E. tenella genome, 28 ropk genes are predicted and could be classified as active (n = 7), inactive (incomplete catalytic triad, n = 12), and non-canonical kinases (active kinase with a modified catalytic triad, n = 9). We characterized the ropk gene expression patterns by real-time quantitative RT-PCR, normalized by parasite housekeeping genes, during the E. tenella life-cycle. Analyzed stages were: non-sporulated oocysts, sporulated oocysts, extracellular and intracellular sporozoites, immature and mature schizonts I, first- and second-generation merozoites, and gametes. Transcription of all those predicted ropk was confirmed. The mean intensity of transcription was higher in extracellular stages and 7–9 ropk were specifically transcribed in merozoites in comparison with sporozoites. Transcriptional profiles of intracellular stages were closely related to each other, suggesting a probable common role of ROPKs in hijacking signaling pathways and immune responses in infected cells. These results provide a solid basis for future functional analysis of ROPK from E. tenella.
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spelling pubmed-83991362021-08-29 Genome-Wide Expression Patterns of Rhoptry Kinases during the Eimeria tenella Life-Cycle Ribeiro E Silva, Adeline Sausset, Alix Bussière, Françoise I. Laurent, Fabrice Lacroix-Lamandé, Sonia Silvestre, Anne Microorganisms Article Kinome from apicomplexan parasites is composed of eukaryotic protein kinases and Apicomplexa specific kinases, such as rhoptry kinases (ROPK). Ropk is a gene family that is known to play important roles in host–pathogen interaction in Toxoplasma gondii but is still poorly described in Eimeria tenella, the parasite responsible for avian coccidiosis worldwide. In the E. tenella genome, 28 ropk genes are predicted and could be classified as active (n = 7), inactive (incomplete catalytic triad, n = 12), and non-canonical kinases (active kinase with a modified catalytic triad, n = 9). We characterized the ropk gene expression patterns by real-time quantitative RT-PCR, normalized by parasite housekeeping genes, during the E. tenella life-cycle. Analyzed stages were: non-sporulated oocysts, sporulated oocysts, extracellular and intracellular sporozoites, immature and mature schizonts I, first- and second-generation merozoites, and gametes. Transcription of all those predicted ropk was confirmed. The mean intensity of transcription was higher in extracellular stages and 7–9 ropk were specifically transcribed in merozoites in comparison with sporozoites. Transcriptional profiles of intracellular stages were closely related to each other, suggesting a probable common role of ROPKs in hijacking signaling pathways and immune responses in infected cells. These results provide a solid basis for future functional analysis of ROPK from E. tenella. MDPI 2021-07-29 /pmc/articles/PMC8399136/ /pubmed/34442701 http://dx.doi.org/10.3390/microorganisms9081621 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ribeiro E Silva, Adeline
Sausset, Alix
Bussière, Françoise I.
Laurent, Fabrice
Lacroix-Lamandé, Sonia
Silvestre, Anne
Genome-Wide Expression Patterns of Rhoptry Kinases during the Eimeria tenella Life-Cycle
title Genome-Wide Expression Patterns of Rhoptry Kinases during the Eimeria tenella Life-Cycle
title_full Genome-Wide Expression Patterns of Rhoptry Kinases during the Eimeria tenella Life-Cycle
title_fullStr Genome-Wide Expression Patterns of Rhoptry Kinases during the Eimeria tenella Life-Cycle
title_full_unstemmed Genome-Wide Expression Patterns of Rhoptry Kinases during the Eimeria tenella Life-Cycle
title_short Genome-Wide Expression Patterns of Rhoptry Kinases during the Eimeria tenella Life-Cycle
title_sort genome-wide expression patterns of rhoptry kinases during the eimeria tenella life-cycle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399136/
https://www.ncbi.nlm.nih.gov/pubmed/34442701
http://dx.doi.org/10.3390/microorganisms9081621
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