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Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy

Peptide synthesis is an area with a wide field of application, from biomedicine to nanotechnology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols generate large vol...

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Autores principales: Guzmán, Fanny, Gauna, Adriana, Roman, Tanya, Luna, Omar, Álvarez, Claudio, Pareja-Barrueto, Claudia, Mercado, Luis, Albericio, Fernando, Cárdenas, Constanza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399505/
https://www.ncbi.nlm.nih.gov/pubmed/34443624
http://dx.doi.org/10.3390/molecules26165035
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author Guzmán, Fanny
Gauna, Adriana
Roman, Tanya
Luna, Omar
Álvarez, Claudio
Pareja-Barrueto, Claudia
Mercado, Luis
Albericio, Fernando
Cárdenas, Constanza
author_facet Guzmán, Fanny
Gauna, Adriana
Roman, Tanya
Luna, Omar
Álvarez, Claudio
Pareja-Barrueto, Claudia
Mercado, Luis
Albericio, Fernando
Cárdenas, Constanza
author_sort Guzmán, Fanny
collection PubMed
description Peptide synthesis is an area with a wide field of application, from biomedicine to nanotechnology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols generate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included. Fifty-two peptides with wide amino acid composition and seven to twenty amino acid residues in length were synthesized in less than three weeks. A clustering analysis was performed, grouping the peptides by physicochemical features. Although a relationship between the overall yield and the physicochemical features of the sequences was not established, the process showed good performance despite sequence diversity. The recycling system allowed to reduce N, N-dimethylformamide usage by 25–30% and reduce the deprotection reagent usage by 50%. This protocol has been optimized for the simultaneous synthesis of a large number of peptide sequences. Additionally, a reagent recycling system was included in the procedure, which turns the process into a framework of circular economy, without affecting the quality of the products obtained.
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spelling pubmed-83995052021-08-29 Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy Guzmán, Fanny Gauna, Adriana Roman, Tanya Luna, Omar Álvarez, Claudio Pareja-Barrueto, Claudia Mercado, Luis Albericio, Fernando Cárdenas, Constanza Molecules Article Peptide synthesis is an area with a wide field of application, from biomedicine to nanotechnology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols generate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included. Fifty-two peptides with wide amino acid composition and seven to twenty amino acid residues in length were synthesized in less than three weeks. A clustering analysis was performed, grouping the peptides by physicochemical features. Although a relationship between the overall yield and the physicochemical features of the sequences was not established, the process showed good performance despite sequence diversity. The recycling system allowed to reduce N, N-dimethylformamide usage by 25–30% and reduce the deprotection reagent usage by 50%. This protocol has been optimized for the simultaneous synthesis of a large number of peptide sequences. Additionally, a reagent recycling system was included in the procedure, which turns the process into a framework of circular economy, without affecting the quality of the products obtained. MDPI 2021-08-19 /pmc/articles/PMC8399505/ /pubmed/34443624 http://dx.doi.org/10.3390/molecules26165035 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guzmán, Fanny
Gauna, Adriana
Roman, Tanya
Luna, Omar
Álvarez, Claudio
Pareja-Barrueto, Claudia
Mercado, Luis
Albericio, Fernando
Cárdenas, Constanza
Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy
title Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy
title_full Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy
title_fullStr Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy
title_full_unstemmed Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy
title_short Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy
title_sort tea bags for fmoc solid-phase peptide synthesis: an example of circular economy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399505/
https://www.ncbi.nlm.nih.gov/pubmed/34443624
http://dx.doi.org/10.3390/molecules26165035
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