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Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy
Peptide synthesis is an area with a wide field of application, from biomedicine to nanotechnology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols generate large vol...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399505/ https://www.ncbi.nlm.nih.gov/pubmed/34443624 http://dx.doi.org/10.3390/molecules26165035 |
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author | Guzmán, Fanny Gauna, Adriana Roman, Tanya Luna, Omar Álvarez, Claudio Pareja-Barrueto, Claudia Mercado, Luis Albericio, Fernando Cárdenas, Constanza |
author_facet | Guzmán, Fanny Gauna, Adriana Roman, Tanya Luna, Omar Álvarez, Claudio Pareja-Barrueto, Claudia Mercado, Luis Albericio, Fernando Cárdenas, Constanza |
author_sort | Guzmán, Fanny |
collection | PubMed |
description | Peptide synthesis is an area with a wide field of application, from biomedicine to nanotechnology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols generate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included. Fifty-two peptides with wide amino acid composition and seven to twenty amino acid residues in length were synthesized in less than three weeks. A clustering analysis was performed, grouping the peptides by physicochemical features. Although a relationship between the overall yield and the physicochemical features of the sequences was not established, the process showed good performance despite sequence diversity. The recycling system allowed to reduce N, N-dimethylformamide usage by 25–30% and reduce the deprotection reagent usage by 50%. This protocol has been optimized for the simultaneous synthesis of a large number of peptide sequences. Additionally, a reagent recycling system was included in the procedure, which turns the process into a framework of circular economy, without affecting the quality of the products obtained. |
format | Online Article Text |
id | pubmed-8399505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83995052021-08-29 Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy Guzmán, Fanny Gauna, Adriana Roman, Tanya Luna, Omar Álvarez, Claudio Pareja-Barrueto, Claudia Mercado, Luis Albericio, Fernando Cárdenas, Constanza Molecules Article Peptide synthesis is an area with a wide field of application, from biomedicine to nanotechnology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols generate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included. Fifty-two peptides with wide amino acid composition and seven to twenty amino acid residues in length were synthesized in less than three weeks. A clustering analysis was performed, grouping the peptides by physicochemical features. Although a relationship between the overall yield and the physicochemical features of the sequences was not established, the process showed good performance despite sequence diversity. The recycling system allowed to reduce N, N-dimethylformamide usage by 25–30% and reduce the deprotection reagent usage by 50%. This protocol has been optimized for the simultaneous synthesis of a large number of peptide sequences. Additionally, a reagent recycling system was included in the procedure, which turns the process into a framework of circular economy, without affecting the quality of the products obtained. MDPI 2021-08-19 /pmc/articles/PMC8399505/ /pubmed/34443624 http://dx.doi.org/10.3390/molecules26165035 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guzmán, Fanny Gauna, Adriana Roman, Tanya Luna, Omar Álvarez, Claudio Pareja-Barrueto, Claudia Mercado, Luis Albericio, Fernando Cárdenas, Constanza Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy |
title | Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy |
title_full | Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy |
title_fullStr | Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy |
title_full_unstemmed | Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy |
title_short | Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy |
title_sort | tea bags for fmoc solid-phase peptide synthesis: an example of circular economy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399505/ https://www.ncbi.nlm.nih.gov/pubmed/34443624 http://dx.doi.org/10.3390/molecules26165035 |
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