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Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds
This study aims to point out the main drawback with respect to the design of simulated body environments. Three media commonly used for the simulation of the identical body environment were selected, i.e., Kokubo’s simulated body fluid that simulates the inorganic component of human blood plasma, hu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399520/ https://www.ncbi.nlm.nih.gov/pubmed/34442910 http://dx.doi.org/10.3390/ma14164388 |
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author | Suchý, Tomáš Bartoš, Martin Sedláček, Radek Šupová, Monika Žaloudková, Margit Martynková, Gražyna Simha Foltán, René |
author_facet | Suchý, Tomáš Bartoš, Martin Sedláček, Radek Šupová, Monika Žaloudková, Margit Martynková, Gražyna Simha Foltán, René |
author_sort | Suchý, Tomáš |
collection | PubMed |
description | This study aims to point out the main drawback with respect to the design of simulated body environments. Three media commonly used for the simulation of the identical body environment were selected, i.e., Kokubo’s simulated body fluid that simulates the inorganic component of human blood plasma, human blood plasma, and phosphate buffer saline. A comparison was performed of the effects of the media on collagen scaffolds. The mechanical and structural effects of the media were determined via the application of compression mechanical tests, the determination of mass loss, and image and micro-CT analyses. The adsorption of various components from the media was characterized employing energy-dispersive spectrometry. The phase composition of the materials before and after exposure was determined using X-ray diffraction. Infrared spectroscopy was employed for the interpretation of changes in the collagen secondary structure. Major differences in terms of the mechanical properties and mass loss were observed between the three media. Conversely, only minor structural changes were detected. Since no general recommendation exists for selecting the simulated body environment, it is necessary to avoid the simplification of the results and, ideally, to utilize alternative methods to describe the various aspects of degradation processes that occur in the media. |
format | Online Article Text |
id | pubmed-8399520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83995202021-08-29 Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds Suchý, Tomáš Bartoš, Martin Sedláček, Radek Šupová, Monika Žaloudková, Margit Martynková, Gražyna Simha Foltán, René Materials (Basel) Article This study aims to point out the main drawback with respect to the design of simulated body environments. Three media commonly used for the simulation of the identical body environment were selected, i.e., Kokubo’s simulated body fluid that simulates the inorganic component of human blood plasma, human blood plasma, and phosphate buffer saline. A comparison was performed of the effects of the media on collagen scaffolds. The mechanical and structural effects of the media were determined via the application of compression mechanical tests, the determination of mass loss, and image and micro-CT analyses. The adsorption of various components from the media was characterized employing energy-dispersive spectrometry. The phase composition of the materials before and after exposure was determined using X-ray diffraction. Infrared spectroscopy was employed for the interpretation of changes in the collagen secondary structure. Major differences in terms of the mechanical properties and mass loss were observed between the three media. Conversely, only minor structural changes were detected. Since no general recommendation exists for selecting the simulated body environment, it is necessary to avoid the simplification of the results and, ideally, to utilize alternative methods to describe the various aspects of degradation processes that occur in the media. MDPI 2021-08-05 /pmc/articles/PMC8399520/ /pubmed/34442910 http://dx.doi.org/10.3390/ma14164388 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Suchý, Tomáš Bartoš, Martin Sedláček, Radek Šupová, Monika Žaloudková, Margit Martynková, Gražyna Simha Foltán, René Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds |
title | Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds |
title_full | Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds |
title_fullStr | Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds |
title_full_unstemmed | Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds |
title_short | Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds |
title_sort | various simulated body fluids lead to significant differences in collagen tissue engineering scaffolds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399520/ https://www.ncbi.nlm.nih.gov/pubmed/34442910 http://dx.doi.org/10.3390/ma14164388 |
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