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Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds

This study aims to point out the main drawback with respect to the design of simulated body environments. Three media commonly used for the simulation of the identical body environment were selected, i.e., Kokubo’s simulated body fluid that simulates the inorganic component of human blood plasma, hu...

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Autores principales: Suchý, Tomáš, Bartoš, Martin, Sedláček, Radek, Šupová, Monika, Žaloudková, Margit, Martynková, Gražyna Simha, Foltán, René
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399520/
https://www.ncbi.nlm.nih.gov/pubmed/34442910
http://dx.doi.org/10.3390/ma14164388
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author Suchý, Tomáš
Bartoš, Martin
Sedláček, Radek
Šupová, Monika
Žaloudková, Margit
Martynková, Gražyna Simha
Foltán, René
author_facet Suchý, Tomáš
Bartoš, Martin
Sedláček, Radek
Šupová, Monika
Žaloudková, Margit
Martynková, Gražyna Simha
Foltán, René
author_sort Suchý, Tomáš
collection PubMed
description This study aims to point out the main drawback with respect to the design of simulated body environments. Three media commonly used for the simulation of the identical body environment were selected, i.e., Kokubo’s simulated body fluid that simulates the inorganic component of human blood plasma, human blood plasma, and phosphate buffer saline. A comparison was performed of the effects of the media on collagen scaffolds. The mechanical and structural effects of the media were determined via the application of compression mechanical tests, the determination of mass loss, and image and micro-CT analyses. The adsorption of various components from the media was characterized employing energy-dispersive spectrometry. The phase composition of the materials before and after exposure was determined using X-ray diffraction. Infrared spectroscopy was employed for the interpretation of changes in the collagen secondary structure. Major differences in terms of the mechanical properties and mass loss were observed between the three media. Conversely, only minor structural changes were detected. Since no general recommendation exists for selecting the simulated body environment, it is necessary to avoid the simplification of the results and, ideally, to utilize alternative methods to describe the various aspects of degradation processes that occur in the media.
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spelling pubmed-83995202021-08-29 Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds Suchý, Tomáš Bartoš, Martin Sedláček, Radek Šupová, Monika Žaloudková, Margit Martynková, Gražyna Simha Foltán, René Materials (Basel) Article This study aims to point out the main drawback with respect to the design of simulated body environments. Three media commonly used for the simulation of the identical body environment were selected, i.e., Kokubo’s simulated body fluid that simulates the inorganic component of human blood plasma, human blood plasma, and phosphate buffer saline. A comparison was performed of the effects of the media on collagen scaffolds. The mechanical and structural effects of the media were determined via the application of compression mechanical tests, the determination of mass loss, and image and micro-CT analyses. The adsorption of various components from the media was characterized employing energy-dispersive spectrometry. The phase composition of the materials before and after exposure was determined using X-ray diffraction. Infrared spectroscopy was employed for the interpretation of changes in the collagen secondary structure. Major differences in terms of the mechanical properties and mass loss were observed between the three media. Conversely, only minor structural changes were detected. Since no general recommendation exists for selecting the simulated body environment, it is necessary to avoid the simplification of the results and, ideally, to utilize alternative methods to describe the various aspects of degradation processes that occur in the media. MDPI 2021-08-05 /pmc/articles/PMC8399520/ /pubmed/34442910 http://dx.doi.org/10.3390/ma14164388 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Suchý, Tomáš
Bartoš, Martin
Sedláček, Radek
Šupová, Monika
Žaloudková, Margit
Martynková, Gražyna Simha
Foltán, René
Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds
title Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds
title_full Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds
title_fullStr Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds
title_full_unstemmed Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds
title_short Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds
title_sort various simulated body fluids lead to significant differences in collagen tissue engineering scaffolds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399520/
https://www.ncbi.nlm.nih.gov/pubmed/34442910
http://dx.doi.org/10.3390/ma14164388
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