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Wasted Biomaterials from Crustaceans as a Compliant Natural Product Regarding Microbiological, Antibacterial Properties and Heavy Metal Content for Reuse in Blue Bioeconomy: A Preliminary Study

The compliance of crab shells traditionally used as a complex natural product for agricultural soil amendment with modern biofertilizers’ quality and safety requirements was investigated. Shells waste from the Blue crab, Callinectes sapidus and the Green crab, Carcinus aestuarii were tested for macr...

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Autores principales: Nekvapil, Fran, Ganea, Iolanda-Veronica, Ciorîță, Alexandra, Hirian, Razvan, Ogresta, Lovro, Glamuzina, Branko, Roba, Carmen, Cintă Pinzaru, Simona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399662/
https://www.ncbi.nlm.nih.gov/pubmed/34443081
http://dx.doi.org/10.3390/ma14164558
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author Nekvapil, Fran
Ganea, Iolanda-Veronica
Ciorîță, Alexandra
Hirian, Razvan
Ogresta, Lovro
Glamuzina, Branko
Roba, Carmen
Cintă Pinzaru, Simona
author_facet Nekvapil, Fran
Ganea, Iolanda-Veronica
Ciorîță, Alexandra
Hirian, Razvan
Ogresta, Lovro
Glamuzina, Branko
Roba, Carmen
Cintă Pinzaru, Simona
author_sort Nekvapil, Fran
collection PubMed
description The compliance of crab shells traditionally used as a complex natural product for agricultural soil amendment with modern biofertilizers’ quality and safety requirements was investigated. Shells waste from the Blue crab, Callinectes sapidus and the Green crab, Carcinus aestuarii were tested for macronutrients, heavy metals, bacteria content, and antimicrobial properties. Such information is crucial for further utilization of the biogenic powders for any composite formulation in added-value by-products. The calcium carbonate-rich hard tissue yield was 52.13% ± 0.015 (mean ± S.D.) and 64.71% ± 0.144 from the blue and green crabs, respectively. The contents of Pb, Ni, Zn, Cr (VI), and Cu were several orders of magnitude below the prescribed limit by EU biofertilizer legislation, with Fe, Mn (not prescribed), and As being the most abundant. The content of As and Cd from the material considered here was within limits. The shells contain no colony-forming units of Salmonella spp. and compliant levels of Escherichia coli; moreover, the shell micro-powder showed dose-dependent growth inhibition of Pseudomonas aeruginosa and Staphylococcus aureus. In summary, the waste crab shells present a complex natural product as plant biofertilizer following the circular economy concepts.
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spelling pubmed-83996622021-08-29 Wasted Biomaterials from Crustaceans as a Compliant Natural Product Regarding Microbiological, Antibacterial Properties and Heavy Metal Content for Reuse in Blue Bioeconomy: A Preliminary Study Nekvapil, Fran Ganea, Iolanda-Veronica Ciorîță, Alexandra Hirian, Razvan Ogresta, Lovro Glamuzina, Branko Roba, Carmen Cintă Pinzaru, Simona Materials (Basel) Article The compliance of crab shells traditionally used as a complex natural product for agricultural soil amendment with modern biofertilizers’ quality and safety requirements was investigated. Shells waste from the Blue crab, Callinectes sapidus and the Green crab, Carcinus aestuarii were tested for macronutrients, heavy metals, bacteria content, and antimicrobial properties. Such information is crucial for further utilization of the biogenic powders for any composite formulation in added-value by-products. The calcium carbonate-rich hard tissue yield was 52.13% ± 0.015 (mean ± S.D.) and 64.71% ± 0.144 from the blue and green crabs, respectively. The contents of Pb, Ni, Zn, Cr (VI), and Cu were several orders of magnitude below the prescribed limit by EU biofertilizer legislation, with Fe, Mn (not prescribed), and As being the most abundant. The content of As and Cd from the material considered here was within limits. The shells contain no colony-forming units of Salmonella spp. and compliant levels of Escherichia coli; moreover, the shell micro-powder showed dose-dependent growth inhibition of Pseudomonas aeruginosa and Staphylococcus aureus. In summary, the waste crab shells present a complex natural product as plant biofertilizer following the circular economy concepts. MDPI 2021-08-13 /pmc/articles/PMC8399662/ /pubmed/34443081 http://dx.doi.org/10.3390/ma14164558 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nekvapil, Fran
Ganea, Iolanda-Veronica
Ciorîță, Alexandra
Hirian, Razvan
Ogresta, Lovro
Glamuzina, Branko
Roba, Carmen
Cintă Pinzaru, Simona
Wasted Biomaterials from Crustaceans as a Compliant Natural Product Regarding Microbiological, Antibacterial Properties and Heavy Metal Content for Reuse in Blue Bioeconomy: A Preliminary Study
title Wasted Biomaterials from Crustaceans as a Compliant Natural Product Regarding Microbiological, Antibacterial Properties and Heavy Metal Content for Reuse in Blue Bioeconomy: A Preliminary Study
title_full Wasted Biomaterials from Crustaceans as a Compliant Natural Product Regarding Microbiological, Antibacterial Properties and Heavy Metal Content for Reuse in Blue Bioeconomy: A Preliminary Study
title_fullStr Wasted Biomaterials from Crustaceans as a Compliant Natural Product Regarding Microbiological, Antibacterial Properties and Heavy Metal Content for Reuse in Blue Bioeconomy: A Preliminary Study
title_full_unstemmed Wasted Biomaterials from Crustaceans as a Compliant Natural Product Regarding Microbiological, Antibacterial Properties and Heavy Metal Content for Reuse in Blue Bioeconomy: A Preliminary Study
title_short Wasted Biomaterials from Crustaceans as a Compliant Natural Product Regarding Microbiological, Antibacterial Properties and Heavy Metal Content for Reuse in Blue Bioeconomy: A Preliminary Study
title_sort wasted biomaterials from crustaceans as a compliant natural product regarding microbiological, antibacterial properties and heavy metal content for reuse in blue bioeconomy: a preliminary study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399662/
https://www.ncbi.nlm.nih.gov/pubmed/34443081
http://dx.doi.org/10.3390/ma14164558
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