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Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms

Biofilm infections are a global public health threat, necessitating new treatment strategies. Biofilm formation also contributes to the development and spread of multidrug-resistant (MDR) bacterial strains. Biofilm-associated chronic infections typically involve colonization by more than one bacteri...

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Autores principales: Makabenta, Jessa Marie V., Park, Jungmi, Li, Cheng-Hsuan, Chattopadhyay, Aritra Nath, Nabawy, Ahmed, Landis, Ryan F., Gupta, Akash, Schmidt-Malan, Suzannah, Patel, Robin, Rotello, Vincent M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399783/
https://www.ncbi.nlm.nih.gov/pubmed/34443542
http://dx.doi.org/10.3390/molecules26164958
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author Makabenta, Jessa Marie V.
Park, Jungmi
Li, Cheng-Hsuan
Chattopadhyay, Aritra Nath
Nabawy, Ahmed
Landis, Ryan F.
Gupta, Akash
Schmidt-Malan, Suzannah
Patel, Robin
Rotello, Vincent M.
author_facet Makabenta, Jessa Marie V.
Park, Jungmi
Li, Cheng-Hsuan
Chattopadhyay, Aritra Nath
Nabawy, Ahmed
Landis, Ryan F.
Gupta, Akash
Schmidt-Malan, Suzannah
Patel, Robin
Rotello, Vincent M.
author_sort Makabenta, Jessa Marie V.
collection PubMed
description Biofilm infections are a global public health threat, necessitating new treatment strategies. Biofilm formation also contributes to the development and spread of multidrug-resistant (MDR) bacterial strains. Biofilm-associated chronic infections typically involve colonization by more than one bacterial species. The co-existence of multiple species of bacteria in biofilms exacerbates therapeutic challenges and can render traditional antibiotics ineffective. Polymeric nanoparticles offer alternative antimicrobial approaches to antibiotics, owing to their tunable physico-chemical properties. Here, we report the efficacy of poly(oxanorborneneimide) (PONI)-based antimicrobial polymeric nanoparticles (PNPs) against multi-species bacterial biofilms. PNPs showed good dual-species biofilm penetration profiles as confirmed by confocal laser scanning microscopy. Broad-spectrum antimicrobial activity was observed, with reduction in both bacterial viability and overall biofilm mass. Further, PNPs displayed minimal fibroblast toxicity and high antimicrobial activity in an in vitro co-culture model comprising fibroblast cells and dual-species biofilms of Escherichia coli and Pseudomonas aeruginosa. This study highlights a potential clinical application of the presented polymeric platform.
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spelling pubmed-83997832021-08-29 Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms Makabenta, Jessa Marie V. Park, Jungmi Li, Cheng-Hsuan Chattopadhyay, Aritra Nath Nabawy, Ahmed Landis, Ryan F. Gupta, Akash Schmidt-Malan, Suzannah Patel, Robin Rotello, Vincent M. Molecules Article Biofilm infections are a global public health threat, necessitating new treatment strategies. Biofilm formation also contributes to the development and spread of multidrug-resistant (MDR) bacterial strains. Biofilm-associated chronic infections typically involve colonization by more than one bacterial species. The co-existence of multiple species of bacteria in biofilms exacerbates therapeutic challenges and can render traditional antibiotics ineffective. Polymeric nanoparticles offer alternative antimicrobial approaches to antibiotics, owing to their tunable physico-chemical properties. Here, we report the efficacy of poly(oxanorborneneimide) (PONI)-based antimicrobial polymeric nanoparticles (PNPs) against multi-species bacterial biofilms. PNPs showed good dual-species biofilm penetration profiles as confirmed by confocal laser scanning microscopy. Broad-spectrum antimicrobial activity was observed, with reduction in both bacterial viability and overall biofilm mass. Further, PNPs displayed minimal fibroblast toxicity and high antimicrobial activity in an in vitro co-culture model comprising fibroblast cells and dual-species biofilms of Escherichia coli and Pseudomonas aeruginosa. This study highlights a potential clinical application of the presented polymeric platform. MDPI 2021-08-16 /pmc/articles/PMC8399783/ /pubmed/34443542 http://dx.doi.org/10.3390/molecules26164958 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Makabenta, Jessa Marie V.
Park, Jungmi
Li, Cheng-Hsuan
Chattopadhyay, Aritra Nath
Nabawy, Ahmed
Landis, Ryan F.
Gupta, Akash
Schmidt-Malan, Suzannah
Patel, Robin
Rotello, Vincent M.
Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms
title Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms
title_full Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms
title_fullStr Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms
title_full_unstemmed Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms
title_short Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms
title_sort polymeric nanoparticles active against dual-species bacterial biofilms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399783/
https://www.ncbi.nlm.nih.gov/pubmed/34443542
http://dx.doi.org/10.3390/molecules26164958
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