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Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens
Antimicrobial peptides are a fundamental component of mollusks’ defense systems, though they remain a thinly investigated subject. Here, infection by Vibrio parahemolyticus triggered a significant increase in antimicrobial activity in oyster plasma. By using PBS-challenged oysters as a control, plas...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399951/ https://www.ncbi.nlm.nih.gov/pubmed/34436258 http://dx.doi.org/10.3390/md19080420 |
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author | Mao, Fan Bao, Yongbo Wong, Nai-Kei Huang, Minwei Liu, Kunna Zhang, Xiangyu Yang, Zhuo Yi, Wenjie Shu, Xiao Xiang, Zhiming Yu, Ziniu Zhang, Yang |
author_facet | Mao, Fan Bao, Yongbo Wong, Nai-Kei Huang, Minwei Liu, Kunna Zhang, Xiangyu Yang, Zhuo Yi, Wenjie Shu, Xiao Xiang, Zhiming Yu, Ziniu Zhang, Yang |
author_sort | Mao, Fan |
collection | PubMed |
description | Antimicrobial peptides are a fundamental component of mollusks’ defense systems, though they remain a thinly investigated subject. Here, infection by Vibrio parahemolyticus triggered a significant increase in antimicrobial activity in oyster plasma. By using PBS-challenged oysters as a control, plasma peptides from immunologically challenged oysters were subjected to peptidomic profiling and in silico data mining to identify bioactive peptides. Thirty-five identified plasma peptides were up-regulated post infection, among which, six up-regulated peptides (URPs) showed a relatively high positive charge. URP20 was validated with significant antibacterial activity. Virtually, URP20 triggered aggregation of bacterial cells, accompanied by their membrane permeabilization. Interestingly, URP20 was found to be active against Gram-positive and Gram-negative foodborne pathogens as well as Candida albicans, with no cytotoxicity to mammalian cells and mice. Our study provides the first large-scale plasma peptidomic dataset that identifies novel bioactive peptides in marine mollusks. Further exploration of peptide diversity in marine invertebrates should prove a fruitful pursuit for designing novel AMPs with broad applications. |
format | Online Article Text |
id | pubmed-8399951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83999512021-08-29 Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens Mao, Fan Bao, Yongbo Wong, Nai-Kei Huang, Minwei Liu, Kunna Zhang, Xiangyu Yang, Zhuo Yi, Wenjie Shu, Xiao Xiang, Zhiming Yu, Ziniu Zhang, Yang Mar Drugs Article Antimicrobial peptides are a fundamental component of mollusks’ defense systems, though they remain a thinly investigated subject. Here, infection by Vibrio parahemolyticus triggered a significant increase in antimicrobial activity in oyster plasma. By using PBS-challenged oysters as a control, plasma peptides from immunologically challenged oysters were subjected to peptidomic profiling and in silico data mining to identify bioactive peptides. Thirty-five identified plasma peptides were up-regulated post infection, among which, six up-regulated peptides (URPs) showed a relatively high positive charge. URP20 was validated with significant antibacterial activity. Virtually, URP20 triggered aggregation of bacterial cells, accompanied by their membrane permeabilization. Interestingly, URP20 was found to be active against Gram-positive and Gram-negative foodborne pathogens as well as Candida albicans, with no cytotoxicity to mammalian cells and mice. Our study provides the first large-scale plasma peptidomic dataset that identifies novel bioactive peptides in marine mollusks. Further exploration of peptide diversity in marine invertebrates should prove a fruitful pursuit for designing novel AMPs with broad applications. MDPI 2021-07-26 /pmc/articles/PMC8399951/ /pubmed/34436258 http://dx.doi.org/10.3390/md19080420 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mao, Fan Bao, Yongbo Wong, Nai-Kei Huang, Minwei Liu, Kunna Zhang, Xiangyu Yang, Zhuo Yi, Wenjie Shu, Xiao Xiang, Zhiming Yu, Ziniu Zhang, Yang Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens |
title | Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens |
title_full | Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens |
title_fullStr | Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens |
title_full_unstemmed | Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens |
title_short | Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens |
title_sort | large-scale plasma peptidomic profiling reveals a novel, nontoxic, crassostrea hongkongensis-derived antimicrobial peptide against foodborne pathogens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399951/ https://www.ncbi.nlm.nih.gov/pubmed/34436258 http://dx.doi.org/10.3390/md19080420 |
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