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Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens

Antimicrobial peptides are a fundamental component of mollusks’ defense systems, though they remain a thinly investigated subject. Here, infection by Vibrio parahemolyticus triggered a significant increase in antimicrobial activity in oyster plasma. By using PBS-challenged oysters as a control, plas...

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Autores principales: Mao, Fan, Bao, Yongbo, Wong, Nai-Kei, Huang, Minwei, Liu, Kunna, Zhang, Xiangyu, Yang, Zhuo, Yi, Wenjie, Shu, Xiao, Xiang, Zhiming, Yu, Ziniu, Zhang, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399951/
https://www.ncbi.nlm.nih.gov/pubmed/34436258
http://dx.doi.org/10.3390/md19080420
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author Mao, Fan
Bao, Yongbo
Wong, Nai-Kei
Huang, Minwei
Liu, Kunna
Zhang, Xiangyu
Yang, Zhuo
Yi, Wenjie
Shu, Xiao
Xiang, Zhiming
Yu, Ziniu
Zhang, Yang
author_facet Mao, Fan
Bao, Yongbo
Wong, Nai-Kei
Huang, Minwei
Liu, Kunna
Zhang, Xiangyu
Yang, Zhuo
Yi, Wenjie
Shu, Xiao
Xiang, Zhiming
Yu, Ziniu
Zhang, Yang
author_sort Mao, Fan
collection PubMed
description Antimicrobial peptides are a fundamental component of mollusks’ defense systems, though they remain a thinly investigated subject. Here, infection by Vibrio parahemolyticus triggered a significant increase in antimicrobial activity in oyster plasma. By using PBS-challenged oysters as a control, plasma peptides from immunologically challenged oysters were subjected to peptidomic profiling and in silico data mining to identify bioactive peptides. Thirty-five identified plasma peptides were up-regulated post infection, among which, six up-regulated peptides (URPs) showed a relatively high positive charge. URP20 was validated with significant antibacterial activity. Virtually, URP20 triggered aggregation of bacterial cells, accompanied by their membrane permeabilization. Interestingly, URP20 was found to be active against Gram-positive and Gram-negative foodborne pathogens as well as Candida albicans, with no cytotoxicity to mammalian cells and mice. Our study provides the first large-scale plasma peptidomic dataset that identifies novel bioactive peptides in marine mollusks. Further exploration of peptide diversity in marine invertebrates should prove a fruitful pursuit for designing novel AMPs with broad applications.
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spelling pubmed-83999512021-08-29 Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens Mao, Fan Bao, Yongbo Wong, Nai-Kei Huang, Minwei Liu, Kunna Zhang, Xiangyu Yang, Zhuo Yi, Wenjie Shu, Xiao Xiang, Zhiming Yu, Ziniu Zhang, Yang Mar Drugs Article Antimicrobial peptides are a fundamental component of mollusks’ defense systems, though they remain a thinly investigated subject. Here, infection by Vibrio parahemolyticus triggered a significant increase in antimicrobial activity in oyster plasma. By using PBS-challenged oysters as a control, plasma peptides from immunologically challenged oysters were subjected to peptidomic profiling and in silico data mining to identify bioactive peptides. Thirty-five identified plasma peptides were up-regulated post infection, among which, six up-regulated peptides (URPs) showed a relatively high positive charge. URP20 was validated with significant antibacterial activity. Virtually, URP20 triggered aggregation of bacterial cells, accompanied by their membrane permeabilization. Interestingly, URP20 was found to be active against Gram-positive and Gram-negative foodborne pathogens as well as Candida albicans, with no cytotoxicity to mammalian cells and mice. Our study provides the first large-scale plasma peptidomic dataset that identifies novel bioactive peptides in marine mollusks. Further exploration of peptide diversity in marine invertebrates should prove a fruitful pursuit for designing novel AMPs with broad applications. MDPI 2021-07-26 /pmc/articles/PMC8399951/ /pubmed/34436258 http://dx.doi.org/10.3390/md19080420 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mao, Fan
Bao, Yongbo
Wong, Nai-Kei
Huang, Minwei
Liu, Kunna
Zhang, Xiangyu
Yang, Zhuo
Yi, Wenjie
Shu, Xiao
Xiang, Zhiming
Yu, Ziniu
Zhang, Yang
Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens
title Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens
title_full Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens
title_fullStr Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens
title_full_unstemmed Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens
title_short Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens
title_sort large-scale plasma peptidomic profiling reveals a novel, nontoxic, crassostrea hongkongensis-derived antimicrobial peptide against foodborne pathogens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399951/
https://www.ncbi.nlm.nih.gov/pubmed/34436258
http://dx.doi.org/10.3390/md19080420
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