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From Water into Sediment—Tracing Freshwater Cyanobacteria via DNA Analyses
Sedimentary ancient DNA-based studies have been used to probe centuries of climate and environmental changes and how they affected cyanobacterial assemblages in temperate lakes. Due to cyanobacteria containing potential bloom-forming and toxin-producing taxa, their approximate reconstruction from se...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400057/ https://www.ncbi.nlm.nih.gov/pubmed/34442857 http://dx.doi.org/10.3390/microorganisms9081778 |
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author | Nwosu, Ebuka Canisius Roeser, Patricia Yang, Sizhong Ganzert, Lars Dellwig, Olaf Pinkerneil, Sylvia Brauer, Achim Dittmann, Elke Wagner, Dirk Liebner, Susanne |
author_facet | Nwosu, Ebuka Canisius Roeser, Patricia Yang, Sizhong Ganzert, Lars Dellwig, Olaf Pinkerneil, Sylvia Brauer, Achim Dittmann, Elke Wagner, Dirk Liebner, Susanne |
author_sort | Nwosu, Ebuka Canisius |
collection | PubMed |
description | Sedimentary ancient DNA-based studies have been used to probe centuries of climate and environmental changes and how they affected cyanobacterial assemblages in temperate lakes. Due to cyanobacteria containing potential bloom-forming and toxin-producing taxa, their approximate reconstruction from sediments is crucial, especially in lakes lacking long-term monitoring data. To extend the resolution of sediment record interpretation, we used high-throughput sequencing, amplicon sequence variant (ASV) analysis, and quantitative PCR to compare pelagic cyanobacterial composition to that in sediment traps (collected monthly) and surface sediments in Lake Tiefer See. Cyanobacterial composition, species richness, and evenness was not significantly different among the pelagic depths, sediment traps and surface sediments (p > 0.05), indicating that the cyanobacteria in the sediments reflected the cyanobacterial assemblage in the water column. However, total cyanobacterial abundances (qPCR) decreased from the metalimnion down the water column. The aggregate-forming (Aphanizomenon) and colony-forming taxa (Snowella) showed pronounced sedimentation. In contrast, Planktothrix was only very poorly represented in sediment traps (meta- and hypolimnion) and surface sediments, despite its highest relative abundance at the thermocline (10 m water depth) during periods of lake stratification (May–October). We conclude that this skewed representation in taxonomic abundances reflects taphonomic processes, which should be considered in future DNA-based paleolimnological investigations. |
format | Online Article Text |
id | pubmed-8400057 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84000572021-08-29 From Water into Sediment—Tracing Freshwater Cyanobacteria via DNA Analyses Nwosu, Ebuka Canisius Roeser, Patricia Yang, Sizhong Ganzert, Lars Dellwig, Olaf Pinkerneil, Sylvia Brauer, Achim Dittmann, Elke Wagner, Dirk Liebner, Susanne Microorganisms Article Sedimentary ancient DNA-based studies have been used to probe centuries of climate and environmental changes and how they affected cyanobacterial assemblages in temperate lakes. Due to cyanobacteria containing potential bloom-forming and toxin-producing taxa, their approximate reconstruction from sediments is crucial, especially in lakes lacking long-term monitoring data. To extend the resolution of sediment record interpretation, we used high-throughput sequencing, amplicon sequence variant (ASV) analysis, and quantitative PCR to compare pelagic cyanobacterial composition to that in sediment traps (collected monthly) and surface sediments in Lake Tiefer See. Cyanobacterial composition, species richness, and evenness was not significantly different among the pelagic depths, sediment traps and surface sediments (p > 0.05), indicating that the cyanobacteria in the sediments reflected the cyanobacterial assemblage in the water column. However, total cyanobacterial abundances (qPCR) decreased from the metalimnion down the water column. The aggregate-forming (Aphanizomenon) and colony-forming taxa (Snowella) showed pronounced sedimentation. In contrast, Planktothrix was only very poorly represented in sediment traps (meta- and hypolimnion) and surface sediments, despite its highest relative abundance at the thermocline (10 m water depth) during periods of lake stratification (May–October). We conclude that this skewed representation in taxonomic abundances reflects taphonomic processes, which should be considered in future DNA-based paleolimnological investigations. MDPI 2021-08-21 /pmc/articles/PMC8400057/ /pubmed/34442857 http://dx.doi.org/10.3390/microorganisms9081778 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nwosu, Ebuka Canisius Roeser, Patricia Yang, Sizhong Ganzert, Lars Dellwig, Olaf Pinkerneil, Sylvia Brauer, Achim Dittmann, Elke Wagner, Dirk Liebner, Susanne From Water into Sediment—Tracing Freshwater Cyanobacteria via DNA Analyses |
title | From Water into Sediment—Tracing Freshwater Cyanobacteria via DNA Analyses |
title_full | From Water into Sediment—Tracing Freshwater Cyanobacteria via DNA Analyses |
title_fullStr | From Water into Sediment—Tracing Freshwater Cyanobacteria via DNA Analyses |
title_full_unstemmed | From Water into Sediment—Tracing Freshwater Cyanobacteria via DNA Analyses |
title_short | From Water into Sediment—Tracing Freshwater Cyanobacteria via DNA Analyses |
title_sort | from water into sediment—tracing freshwater cyanobacteria via dna analyses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400057/ https://www.ncbi.nlm.nih.gov/pubmed/34442857 http://dx.doi.org/10.3390/microorganisms9081778 |
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