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Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)

Cell bioprinting technologies aim to fabricate tissuelike constructs by delivering biomaterials layer-by-layer. Bioprinted constructs can reduce the use of animals in drug development and hold promise for addressing the shortage of organs for transplants. Here, we sought to validate the feasibility...

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Autores principales: Roversi, Katiane, Ebrahimi Orimi, Hamid, Falchetti, Marcelo, Lummertz da Rocha, Edroaldo, Talbot, Sebastien, Boutopoulos, Christos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400135/
https://www.ncbi.nlm.nih.gov/pubmed/34442487
http://dx.doi.org/10.3390/mi12080865
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author Roversi, Katiane
Ebrahimi Orimi, Hamid
Falchetti, Marcelo
Lummertz da Rocha, Edroaldo
Talbot, Sebastien
Boutopoulos, Christos
author_facet Roversi, Katiane
Ebrahimi Orimi, Hamid
Falchetti, Marcelo
Lummertz da Rocha, Edroaldo
Talbot, Sebastien
Boutopoulos, Christos
author_sort Roversi, Katiane
collection PubMed
description Cell bioprinting technologies aim to fabricate tissuelike constructs by delivering biomaterials layer-by-layer. Bioprinted constructs can reduce the use of animals in drug development and hold promise for addressing the shortage of organs for transplants. Here, we sought to validate the feasibility of bioprinting primary adult sensory neurons using a newly developed laser-assisted cell bioprinting technology, known as Laser-Induced Side Transfer (LIST). We used dorsal root ganglion neurons (DRG; cell bodies of somatosensory neurons) to prepare our bioink. DRG-laden- droplets were printed on fibrin-coated coverslips and their viability, calcium kinetics, neuropeptides release, and neurite outgrowth were measured. The transcriptome of the neurons was sequenced. We found that LIST-printed neurons maintain high viability (Printed: 86%, Control: 87% on average) and their capacity to release neuropeptides (Printed CGRP: 130 pg/mL, Control CGRP: 146 pg/mL). In addition, LIST-printed neurons do not show differences in the expressed genes compared to control neurons. However, in printed neurons, we found compromised neurite outgrowth and lower sensitivity to the ligand of the TRPV1 channel, capsaicin. In conclusion, LIST-printed neurons maintain high viability and marginal functionality losses. Overall, this work paves the way for bioprinting functional 2D neuron assays.
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spelling pubmed-84001352021-08-29 Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST) Roversi, Katiane Ebrahimi Orimi, Hamid Falchetti, Marcelo Lummertz da Rocha, Edroaldo Talbot, Sebastien Boutopoulos, Christos Micromachines (Basel) Article Cell bioprinting technologies aim to fabricate tissuelike constructs by delivering biomaterials layer-by-layer. Bioprinted constructs can reduce the use of animals in drug development and hold promise for addressing the shortage of organs for transplants. Here, we sought to validate the feasibility of bioprinting primary adult sensory neurons using a newly developed laser-assisted cell bioprinting technology, known as Laser-Induced Side Transfer (LIST). We used dorsal root ganglion neurons (DRG; cell bodies of somatosensory neurons) to prepare our bioink. DRG-laden- droplets were printed on fibrin-coated coverslips and their viability, calcium kinetics, neuropeptides release, and neurite outgrowth were measured. The transcriptome of the neurons was sequenced. We found that LIST-printed neurons maintain high viability (Printed: 86%, Control: 87% on average) and their capacity to release neuropeptides (Printed CGRP: 130 pg/mL, Control CGRP: 146 pg/mL). In addition, LIST-printed neurons do not show differences in the expressed genes compared to control neurons. However, in printed neurons, we found compromised neurite outgrowth and lower sensitivity to the ligand of the TRPV1 channel, capsaicin. In conclusion, LIST-printed neurons maintain high viability and marginal functionality losses. Overall, this work paves the way for bioprinting functional 2D neuron assays. MDPI 2021-07-23 /pmc/articles/PMC8400135/ /pubmed/34442487 http://dx.doi.org/10.3390/mi12080865 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Roversi, Katiane
Ebrahimi Orimi, Hamid
Falchetti, Marcelo
Lummertz da Rocha, Edroaldo
Talbot, Sebastien
Boutopoulos, Christos
Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)
title Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)
title_full Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)
title_fullStr Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)
title_full_unstemmed Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)
title_short Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)
title_sort bioprinting of adult dorsal root ganglion (drg) neurons using laser-induced side transfer (list)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400135/
https://www.ncbi.nlm.nih.gov/pubmed/34442487
http://dx.doi.org/10.3390/mi12080865
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