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A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain

In the present study, a HPLC/DAD method was set up to allow for the determination and quantification of malondialdehyde (MDA) in the brain of rodents (rats). Chromatographic separation was achieved on Supelcosil LC-18 (3 μm) SUPELCO Column 3.3 cm × 4.6 mm and Supelco Column Saver 0.5 μm filter by us...

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Autores principales: Jîtcă, George, Fogarasi, Erzsébet, Ősz, Bianca-Eugenia, Vari, Camil Eugen, Tero-Vescan, Amelia, Miklos, Amalia, Bătrînu, Mădălina-Georgiana, Rusz, Carmen Maria, Croitoru, Mircea Dumitru, Dogaru, Maria Titica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400166/
https://www.ncbi.nlm.nih.gov/pubmed/34443656
http://dx.doi.org/10.3390/molecules26165066
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author Jîtcă, George
Fogarasi, Erzsébet
Ősz, Bianca-Eugenia
Vari, Camil Eugen
Tero-Vescan, Amelia
Miklos, Amalia
Bătrînu, Mădălina-Georgiana
Rusz, Carmen Maria
Croitoru, Mircea Dumitru
Dogaru, Maria Titica
author_facet Jîtcă, George
Fogarasi, Erzsébet
Ősz, Bianca-Eugenia
Vari, Camil Eugen
Tero-Vescan, Amelia
Miklos, Amalia
Bătrînu, Mădălina-Georgiana
Rusz, Carmen Maria
Croitoru, Mircea Dumitru
Dogaru, Maria Titica
author_sort Jîtcă, George
collection PubMed
description In the present study, a HPLC/DAD method was set up to allow for the determination and quantification of malondialdehyde (MDA) in the brain of rodents (rats). Chromatographic separation was achieved on Supelcosil LC-18 (3 μm) SUPELCO Column 3.3 cm × 4.6 mm and Supelco Column Saver 0.5 μm filter by using a mobile phase acetonitrile (A) and phosphate buffer (20 mM, pH = 6) (B). Isocratic elution was 14% for (A) and 86% for (B). The injection volume (loop mode) was 100 μL with an analysis time of 1.5 min. Flow rate was set at 1 mL/min. The eluted compound was detected at 532 nm by a DAD detector by keeping the column oven at room temperature. The results indicated that the method has good linearity in the range of 0.2–20 μg/g. Both intra- and inter-day precision, expressed as RSD, were ≤15% and the accuracies ranged between ±15%. The lower limit of quantification (LLOQ), stability, and robustness were evaluated and satisfied the validation criteria. The method was successfully applied in a study of chronic toxicology following different treatment regimens with haloperidol and metformin.
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spelling pubmed-84001662021-08-29 A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain Jîtcă, George Fogarasi, Erzsébet Ősz, Bianca-Eugenia Vari, Camil Eugen Tero-Vescan, Amelia Miklos, Amalia Bătrînu, Mădălina-Georgiana Rusz, Carmen Maria Croitoru, Mircea Dumitru Dogaru, Maria Titica Molecules Article In the present study, a HPLC/DAD method was set up to allow for the determination and quantification of malondialdehyde (MDA) in the brain of rodents (rats). Chromatographic separation was achieved on Supelcosil LC-18 (3 μm) SUPELCO Column 3.3 cm × 4.6 mm and Supelco Column Saver 0.5 μm filter by using a mobile phase acetonitrile (A) and phosphate buffer (20 mM, pH = 6) (B). Isocratic elution was 14% for (A) and 86% for (B). The injection volume (loop mode) was 100 μL with an analysis time of 1.5 min. Flow rate was set at 1 mL/min. The eluted compound was detected at 532 nm by a DAD detector by keeping the column oven at room temperature. The results indicated that the method has good linearity in the range of 0.2–20 μg/g. Both intra- and inter-day precision, expressed as RSD, were ≤15% and the accuracies ranged between ±15%. The lower limit of quantification (LLOQ), stability, and robustness were evaluated and satisfied the validation criteria. The method was successfully applied in a study of chronic toxicology following different treatment regimens with haloperidol and metformin. MDPI 2021-08-21 /pmc/articles/PMC8400166/ /pubmed/34443656 http://dx.doi.org/10.3390/molecules26165066 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jîtcă, George
Fogarasi, Erzsébet
Ősz, Bianca-Eugenia
Vari, Camil Eugen
Tero-Vescan, Amelia
Miklos, Amalia
Bătrînu, Mădălina-Georgiana
Rusz, Carmen Maria
Croitoru, Mircea Dumitru
Dogaru, Maria Titica
A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain
title A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain
title_full A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain
title_fullStr A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain
title_full_unstemmed A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain
title_short A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain
title_sort simple hplc/dad method validation for the quantification of malondialdehyde in rodent’s brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400166/
https://www.ncbi.nlm.nih.gov/pubmed/34443656
http://dx.doi.org/10.3390/molecules26165066
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