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A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain
In the present study, a HPLC/DAD method was set up to allow for the determination and quantification of malondialdehyde (MDA) in the brain of rodents (rats). Chromatographic separation was achieved on Supelcosil LC-18 (3 μm) SUPELCO Column 3.3 cm × 4.6 mm and Supelco Column Saver 0.5 μm filter by us...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400166/ https://www.ncbi.nlm.nih.gov/pubmed/34443656 http://dx.doi.org/10.3390/molecules26165066 |
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author | Jîtcă, George Fogarasi, Erzsébet Ősz, Bianca-Eugenia Vari, Camil Eugen Tero-Vescan, Amelia Miklos, Amalia Bătrînu, Mădălina-Georgiana Rusz, Carmen Maria Croitoru, Mircea Dumitru Dogaru, Maria Titica |
author_facet | Jîtcă, George Fogarasi, Erzsébet Ősz, Bianca-Eugenia Vari, Camil Eugen Tero-Vescan, Amelia Miklos, Amalia Bătrînu, Mădălina-Georgiana Rusz, Carmen Maria Croitoru, Mircea Dumitru Dogaru, Maria Titica |
author_sort | Jîtcă, George |
collection | PubMed |
description | In the present study, a HPLC/DAD method was set up to allow for the determination and quantification of malondialdehyde (MDA) in the brain of rodents (rats). Chromatographic separation was achieved on Supelcosil LC-18 (3 μm) SUPELCO Column 3.3 cm × 4.6 mm and Supelco Column Saver 0.5 μm filter by using a mobile phase acetonitrile (A) and phosphate buffer (20 mM, pH = 6) (B). Isocratic elution was 14% for (A) and 86% for (B). The injection volume (loop mode) was 100 μL with an analysis time of 1.5 min. Flow rate was set at 1 mL/min. The eluted compound was detected at 532 nm by a DAD detector by keeping the column oven at room temperature. The results indicated that the method has good linearity in the range of 0.2–20 μg/g. Both intra- and inter-day precision, expressed as RSD, were ≤15% and the accuracies ranged between ±15%. The lower limit of quantification (LLOQ), stability, and robustness were evaluated and satisfied the validation criteria. The method was successfully applied in a study of chronic toxicology following different treatment regimens with haloperidol and metformin. |
format | Online Article Text |
id | pubmed-8400166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84001662021-08-29 A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain Jîtcă, George Fogarasi, Erzsébet Ősz, Bianca-Eugenia Vari, Camil Eugen Tero-Vescan, Amelia Miklos, Amalia Bătrînu, Mădălina-Georgiana Rusz, Carmen Maria Croitoru, Mircea Dumitru Dogaru, Maria Titica Molecules Article In the present study, a HPLC/DAD method was set up to allow for the determination and quantification of malondialdehyde (MDA) in the brain of rodents (rats). Chromatographic separation was achieved on Supelcosil LC-18 (3 μm) SUPELCO Column 3.3 cm × 4.6 mm and Supelco Column Saver 0.5 μm filter by using a mobile phase acetonitrile (A) and phosphate buffer (20 mM, pH = 6) (B). Isocratic elution was 14% for (A) and 86% for (B). The injection volume (loop mode) was 100 μL with an analysis time of 1.5 min. Flow rate was set at 1 mL/min. The eluted compound was detected at 532 nm by a DAD detector by keeping the column oven at room temperature. The results indicated that the method has good linearity in the range of 0.2–20 μg/g. Both intra- and inter-day precision, expressed as RSD, were ≤15% and the accuracies ranged between ±15%. The lower limit of quantification (LLOQ), stability, and robustness were evaluated and satisfied the validation criteria. The method was successfully applied in a study of chronic toxicology following different treatment regimens with haloperidol and metformin. MDPI 2021-08-21 /pmc/articles/PMC8400166/ /pubmed/34443656 http://dx.doi.org/10.3390/molecules26165066 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jîtcă, George Fogarasi, Erzsébet Ősz, Bianca-Eugenia Vari, Camil Eugen Tero-Vescan, Amelia Miklos, Amalia Bătrînu, Mădălina-Georgiana Rusz, Carmen Maria Croitoru, Mircea Dumitru Dogaru, Maria Titica A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain |
title | A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain |
title_full | A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain |
title_fullStr | A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain |
title_full_unstemmed | A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain |
title_short | A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain |
title_sort | simple hplc/dad method validation for the quantification of malondialdehyde in rodent’s brain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400166/ https://www.ncbi.nlm.nih.gov/pubmed/34443656 http://dx.doi.org/10.3390/molecules26165066 |
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