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Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets

Limosilactobacillus reuteri and Lactiplantibacillus plantarum strains, previously isolated from weaned piglets, were considered for the evaluation of their adhesive characteristics. Lactobacilli were treated with LiCl in order to remove the surface protein layer, and probiotic activity was compared...

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Autores principales: Dell’Anno, Matteo, Giromini, Carlotta, Reggi, Serena, Cavalleri, Mariagrazia, Moscatelli, Alessandra, Onelli, Elisabetta, Rebucci, Raffaella, Sundaram, Tamil Selvi, Coranelli, Simona, Spalletta, Ambra, Baldi, Antonella, Rossi, Luciana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400209/
https://www.ncbi.nlm.nih.gov/pubmed/34442665
http://dx.doi.org/10.3390/microorganisms9081587
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author Dell’Anno, Matteo
Giromini, Carlotta
Reggi, Serena
Cavalleri, Mariagrazia
Moscatelli, Alessandra
Onelli, Elisabetta
Rebucci, Raffaella
Sundaram, Tamil Selvi
Coranelli, Simona
Spalletta, Ambra
Baldi, Antonella
Rossi, Luciana
author_facet Dell’Anno, Matteo
Giromini, Carlotta
Reggi, Serena
Cavalleri, Mariagrazia
Moscatelli, Alessandra
Onelli, Elisabetta
Rebucci, Raffaella
Sundaram, Tamil Selvi
Coranelli, Simona
Spalletta, Ambra
Baldi, Antonella
Rossi, Luciana
author_sort Dell’Anno, Matteo
collection PubMed
description Limosilactobacillus reuteri and Lactiplantibacillus plantarum strains, previously isolated from weaned piglets, were considered for the evaluation of their adhesive characteristics. Lactobacilli were treated with LiCl in order to remove the surface protein layer, and probiotic activity was compared with those of untreated strains. The autoaggregation, co-aggregation to E. coli F18+, and adhesive abilities of LiCl-treated Limosilactobacillus reuteri and Lactiplantibacillus plantarum were significantly inhibited (p < 0.05) compared with the respective untreated strain. The hydrophobic and basic phenotypes were observed due to the strong affinity to chloroform and low adherence to ethyl acetate. In particular, L. plantarum showed higher hydrophobicity compared to L. reuteri, which may reflect their different colonizing ability. After treatment with LiCl to remove surface proteins, the adherence capabilities of L. reuteri and L. casei on IPEC-J2 cells decreased significantly (p < 0.001) and L. reuteri adhered more frequently. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that both L. reuteri and L. plantarum had several bands ranging from 20 to 100 kDa. Two-dimensional gel electrophoresis showed an acidic profile of the surface-layer polypeptides for both bacterial strains, and more studies are needed to characterize their profile and functions. The results confirm the pivotal role of surface proteins in the probiotic potential of L. reuteri and L. plantarum.
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spelling pubmed-84002092021-08-29 Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets Dell’Anno, Matteo Giromini, Carlotta Reggi, Serena Cavalleri, Mariagrazia Moscatelli, Alessandra Onelli, Elisabetta Rebucci, Raffaella Sundaram, Tamil Selvi Coranelli, Simona Spalletta, Ambra Baldi, Antonella Rossi, Luciana Microorganisms Article Limosilactobacillus reuteri and Lactiplantibacillus plantarum strains, previously isolated from weaned piglets, were considered for the evaluation of their adhesive characteristics. Lactobacilli were treated with LiCl in order to remove the surface protein layer, and probiotic activity was compared with those of untreated strains. The autoaggregation, co-aggregation to E. coli F18+, and adhesive abilities of LiCl-treated Limosilactobacillus reuteri and Lactiplantibacillus plantarum were significantly inhibited (p < 0.05) compared with the respective untreated strain. The hydrophobic and basic phenotypes were observed due to the strong affinity to chloroform and low adherence to ethyl acetate. In particular, L. plantarum showed higher hydrophobicity compared to L. reuteri, which may reflect their different colonizing ability. After treatment with LiCl to remove surface proteins, the adherence capabilities of L. reuteri and L. casei on IPEC-J2 cells decreased significantly (p < 0.001) and L. reuteri adhered more frequently. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that both L. reuteri and L. plantarum had several bands ranging from 20 to 100 kDa. Two-dimensional gel electrophoresis showed an acidic profile of the surface-layer polypeptides for both bacterial strains, and more studies are needed to characterize their profile and functions. The results confirm the pivotal role of surface proteins in the probiotic potential of L. reuteri and L. plantarum. MDPI 2021-07-26 /pmc/articles/PMC8400209/ /pubmed/34442665 http://dx.doi.org/10.3390/microorganisms9081587 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dell’Anno, Matteo
Giromini, Carlotta
Reggi, Serena
Cavalleri, Mariagrazia
Moscatelli, Alessandra
Onelli, Elisabetta
Rebucci, Raffaella
Sundaram, Tamil Selvi
Coranelli, Simona
Spalletta, Ambra
Baldi, Antonella
Rossi, Luciana
Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets
title Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets
title_full Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets
title_fullStr Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets
title_full_unstemmed Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets
title_short Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets
title_sort evaluation of adhesive characteristics of l. plantarum and l. reuteri isolated from weaned piglets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400209/
https://www.ncbi.nlm.nih.gov/pubmed/34442665
http://dx.doi.org/10.3390/microorganisms9081587
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