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Laser-Induced Graphene-Based Enzymatic Biosensor for Glucose Detection
Laser-induced graphene (LIG) has recently been receiving increasing attention due to its simple fabrication and low cost. This study reports a flexible laser-induced graphene-based electrochemical biosensor fabricated on a polymer substrate by the laser direct engraving process. For this purpose, a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400493/ https://www.ncbi.nlm.nih.gov/pubmed/34451332 http://dx.doi.org/10.3390/polym13162795 |
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author | Settu, Kalpana Chiu, Pin-Tzu Huang, Yu-Ming |
author_facet | Settu, Kalpana Chiu, Pin-Tzu Huang, Yu-Ming |
author_sort | Settu, Kalpana |
collection | PubMed |
description | Laser-induced graphene (LIG) has recently been receiving increasing attention due to its simple fabrication and low cost. This study reports a flexible laser-induced graphene-based electrochemical biosensor fabricated on a polymer substrate by the laser direct engraving process. For this purpose, a 450 nm UV laser was employed to produce a laser-induced graphene electrode (LIGE) on a polyimide substrate. After the laser engraving of LIGE, the chitosan–glucose oxidase (GOx) composite was immobilized on the LIGE surface to develop the biosensor for glucose detection. It was observed that the developed LIGE biosensor exhibited good amperometric responses toward glucose detection over a wide linear range up to 8 mM. The GOx/chitosan-modified LIGE biosensor showed high sensitivity of 43.15 µA mM(−1) cm(−2) with a detection limit of 0.431 mM. The interference studies performed with some possible interfering compounds such as ascorbic acid, uric acid, and urea exhibited no interference as there was no difference observed in the amperometric glucose detection. It was suggested that the LIGE-based biosensor proposed herein was easy to prepare and could be used for low-cost, rapid, and sensitive/selective glucose detection. |
format | Online Article Text |
id | pubmed-8400493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84004932021-08-29 Laser-Induced Graphene-Based Enzymatic Biosensor for Glucose Detection Settu, Kalpana Chiu, Pin-Tzu Huang, Yu-Ming Polymers (Basel) Article Laser-induced graphene (LIG) has recently been receiving increasing attention due to its simple fabrication and low cost. This study reports a flexible laser-induced graphene-based electrochemical biosensor fabricated on a polymer substrate by the laser direct engraving process. For this purpose, a 450 nm UV laser was employed to produce a laser-induced graphene electrode (LIGE) on a polyimide substrate. After the laser engraving of LIGE, the chitosan–glucose oxidase (GOx) composite was immobilized on the LIGE surface to develop the biosensor for glucose detection. It was observed that the developed LIGE biosensor exhibited good amperometric responses toward glucose detection over a wide linear range up to 8 mM. The GOx/chitosan-modified LIGE biosensor showed high sensitivity of 43.15 µA mM(−1) cm(−2) with a detection limit of 0.431 mM. The interference studies performed with some possible interfering compounds such as ascorbic acid, uric acid, and urea exhibited no interference as there was no difference observed in the amperometric glucose detection. It was suggested that the LIGE-based biosensor proposed herein was easy to prepare and could be used for low-cost, rapid, and sensitive/selective glucose detection. MDPI 2021-08-20 /pmc/articles/PMC8400493/ /pubmed/34451332 http://dx.doi.org/10.3390/polym13162795 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Settu, Kalpana Chiu, Pin-Tzu Huang, Yu-Ming Laser-Induced Graphene-Based Enzymatic Biosensor for Glucose Detection |
title | Laser-Induced Graphene-Based Enzymatic Biosensor for Glucose Detection |
title_full | Laser-Induced Graphene-Based Enzymatic Biosensor for Glucose Detection |
title_fullStr | Laser-Induced Graphene-Based Enzymatic Biosensor for Glucose Detection |
title_full_unstemmed | Laser-Induced Graphene-Based Enzymatic Biosensor for Glucose Detection |
title_short | Laser-Induced Graphene-Based Enzymatic Biosensor for Glucose Detection |
title_sort | laser-induced graphene-based enzymatic biosensor for glucose detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400493/ https://www.ncbi.nlm.nih.gov/pubmed/34451332 http://dx.doi.org/10.3390/polym13162795 |
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