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Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins

In this study, we investigated the properties of proteolytic enzymes of two species of Aspergillus, Aspergillus flavus 1 (with a high degree of pathogenicity) and Aspergillus ochraceus L-1 (a conditional pathogen), and their effects on various components of the hemostasis system (in vitro) in the ca...

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Autores principales: Osmolovskiy, Alexander A., Schmidt, Laura, Orekhova, Anastasia V., Komarevtsev, Sergey K., Kreyer, Valeriana G., Shabunin, Sergey V., Egorov, Nikolay S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400497/
https://www.ncbi.nlm.nih.gov/pubmed/34440526
http://dx.doi.org/10.3390/life11080782
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author Osmolovskiy, Alexander A.
Schmidt, Laura
Orekhova, Anastasia V.
Komarevtsev, Sergey K.
Kreyer, Valeriana G.
Shabunin, Sergey V.
Egorov, Nikolay S.
author_facet Osmolovskiy, Alexander A.
Schmidt, Laura
Orekhova, Anastasia V.
Komarevtsev, Sergey K.
Kreyer, Valeriana G.
Shabunin, Sergey V.
Egorov, Nikolay S.
author_sort Osmolovskiy, Alexander A.
collection PubMed
description In this study, we investigated the properties of proteolytic enzymes of two species of Aspergillus, Aspergillus flavus 1 (with a high degree of pathogenicity) and Aspergillus ochraceus L-1 (a conditional pathogen), and their effects on various components of the hemostasis system (in vitro) in the case of their penetration into the bloodstream. We showed that micromycete proteases were highly active in cleaving both globular (albuminolysis) and fibrillar (fibrin) proteins, and, to varying degrees, they could coagulate the plasma of humans and animals (due to proteolysis of factors of the blood coagulation cascade) but were not able to coagulate fibrinogen. The proteases of both Aspergillus fully hydrolyzed thrombi in 120–180 min. Micromycetes did not show hemolytic activity but were able to break down hemoglobin.
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spelling pubmed-84004972021-08-29 Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins Osmolovskiy, Alexander A. Schmidt, Laura Orekhova, Anastasia V. Komarevtsev, Sergey K. Kreyer, Valeriana G. Shabunin, Sergey V. Egorov, Nikolay S. Life (Basel) Article In this study, we investigated the properties of proteolytic enzymes of two species of Aspergillus, Aspergillus flavus 1 (with a high degree of pathogenicity) and Aspergillus ochraceus L-1 (a conditional pathogen), and their effects on various components of the hemostasis system (in vitro) in the case of their penetration into the bloodstream. We showed that micromycete proteases were highly active in cleaving both globular (albuminolysis) and fibrillar (fibrin) proteins, and, to varying degrees, they could coagulate the plasma of humans and animals (due to proteolysis of factors of the blood coagulation cascade) but were not able to coagulate fibrinogen. The proteases of both Aspergillus fully hydrolyzed thrombi in 120–180 min. Micromycetes did not show hemolytic activity but were able to break down hemoglobin. MDPI 2021-08-02 /pmc/articles/PMC8400497/ /pubmed/34440526 http://dx.doi.org/10.3390/life11080782 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Osmolovskiy, Alexander A.
Schmidt, Laura
Orekhova, Anastasia V.
Komarevtsev, Sergey K.
Kreyer, Valeriana G.
Shabunin, Sergey V.
Egorov, Nikolay S.
Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins
title Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins
title_full Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins
title_fullStr Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins
title_full_unstemmed Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins
title_short Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins
title_sort action of extracellular proteases of aspergillus flavus and aspergillus ochraceus micromycetes on plasma hemostasis proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400497/
https://www.ncbi.nlm.nih.gov/pubmed/34440526
http://dx.doi.org/10.3390/life11080782
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