Cargando…

Double-Antibody Sandwich Immunoassay and Plasmonic Coupling Synergistically Improved Long-Range SPR Biosensor with Low Detection Limit

A long-range surface plasmonic resonance (LR-SPR) biosensor modified with double-antibody sandwich immunoassay and plasmonic coupling is demonstrated for human-immunoglobulin G detection with a low limit of detection (LOD). The double-antibody sandwich immunoassay dramatically changes the average re...

Descripción completa

Detalles Bibliográficos
Autores principales: Jing, Jianying, Liu, Kun, Jiang, Junfeng, Xu, Tianhua, Wang, Shuang, Ma, Jinying, Zhang, Zhao, Zhang, Wenlin, Liu, Tiegen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400597/
https://www.ncbi.nlm.nih.gov/pubmed/34443967
http://dx.doi.org/10.3390/nano11082137
_version_ 1783745352451489792
author Jing, Jianying
Liu, Kun
Jiang, Junfeng
Xu, Tianhua
Wang, Shuang
Ma, Jinying
Zhang, Zhao
Zhang, Wenlin
Liu, Tiegen
author_facet Jing, Jianying
Liu, Kun
Jiang, Junfeng
Xu, Tianhua
Wang, Shuang
Ma, Jinying
Zhang, Zhao
Zhang, Wenlin
Liu, Tiegen
author_sort Jing, Jianying
collection PubMed
description A long-range surface plasmonic resonance (LR-SPR) biosensor modified with double-antibody sandwich immunoassay and plasmonic coupling is demonstrated for human-immunoglobulin G detection with a low limit of detection (LOD). The double-antibody sandwich immunoassay dramatically changes the average refractive index of the medium layer on the sensor surface. The near-field electron coupling between the localized surface plasmon and the long-range surface plasmon leads to a significant perturbation of the evanescent field. The large penetration depth and the long propagation distance of the long-range surface plasmonic waves facilitate the LR-SPR sensor in the detection of biological macromolecules. The unique light absorption characteristic of the nanocomposite material in the sensor provides the in situ self-compensation for the disturbance. Therefore, besides the inherent advantages of optical fiber sensors, the developed biosensor can realize the detection of biomolecules with high sensitivity, low LOD and high accuracy and reliability. Experimental results demonstrate that the LOD of the biosensor is as low as 0.11 μg/mL in the detection of the phosphate-buffered saline sample, and the spike-and-repetition rate is 105.56% in the detection of the real serum sample, which partly shows the practicability of the biosensor. This indicates that the LR-SPR biosensor provides better response compared with existing similar sensors and can be regarded as a valuable method for biochemical analysis and disease detection.
format Online
Article
Text
id pubmed-8400597
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84005972021-08-29 Double-Antibody Sandwich Immunoassay and Plasmonic Coupling Synergistically Improved Long-Range SPR Biosensor with Low Detection Limit Jing, Jianying Liu, Kun Jiang, Junfeng Xu, Tianhua Wang, Shuang Ma, Jinying Zhang, Zhao Zhang, Wenlin Liu, Tiegen Nanomaterials (Basel) Article A long-range surface plasmonic resonance (LR-SPR) biosensor modified with double-antibody sandwich immunoassay and plasmonic coupling is demonstrated for human-immunoglobulin G detection with a low limit of detection (LOD). The double-antibody sandwich immunoassay dramatically changes the average refractive index of the medium layer on the sensor surface. The near-field electron coupling between the localized surface plasmon and the long-range surface plasmon leads to a significant perturbation of the evanescent field. The large penetration depth and the long propagation distance of the long-range surface plasmonic waves facilitate the LR-SPR sensor in the detection of biological macromolecules. The unique light absorption characteristic of the nanocomposite material in the sensor provides the in situ self-compensation for the disturbance. Therefore, besides the inherent advantages of optical fiber sensors, the developed biosensor can realize the detection of biomolecules with high sensitivity, low LOD and high accuracy and reliability. Experimental results demonstrate that the LOD of the biosensor is as low as 0.11 μg/mL in the detection of the phosphate-buffered saline sample, and the spike-and-repetition rate is 105.56% in the detection of the real serum sample, which partly shows the practicability of the biosensor. This indicates that the LR-SPR biosensor provides better response compared with existing similar sensors and can be regarded as a valuable method for biochemical analysis and disease detection. MDPI 2021-08-22 /pmc/articles/PMC8400597/ /pubmed/34443967 http://dx.doi.org/10.3390/nano11082137 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jing, Jianying
Liu, Kun
Jiang, Junfeng
Xu, Tianhua
Wang, Shuang
Ma, Jinying
Zhang, Zhao
Zhang, Wenlin
Liu, Tiegen
Double-Antibody Sandwich Immunoassay and Plasmonic Coupling Synergistically Improved Long-Range SPR Biosensor with Low Detection Limit
title Double-Antibody Sandwich Immunoassay and Plasmonic Coupling Synergistically Improved Long-Range SPR Biosensor with Low Detection Limit
title_full Double-Antibody Sandwich Immunoassay and Plasmonic Coupling Synergistically Improved Long-Range SPR Biosensor with Low Detection Limit
title_fullStr Double-Antibody Sandwich Immunoassay and Plasmonic Coupling Synergistically Improved Long-Range SPR Biosensor with Low Detection Limit
title_full_unstemmed Double-Antibody Sandwich Immunoassay and Plasmonic Coupling Synergistically Improved Long-Range SPR Biosensor with Low Detection Limit
title_short Double-Antibody Sandwich Immunoassay and Plasmonic Coupling Synergistically Improved Long-Range SPR Biosensor with Low Detection Limit
title_sort double-antibody sandwich immunoassay and plasmonic coupling synergistically improved long-range spr biosensor with low detection limit
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400597/
https://www.ncbi.nlm.nih.gov/pubmed/34443967
http://dx.doi.org/10.3390/nano11082137
work_keys_str_mv AT jingjianying doubleantibodysandwichimmunoassayandplasmoniccouplingsynergisticallyimprovedlongrangesprbiosensorwithlowdetectionlimit
AT liukun doubleantibodysandwichimmunoassayandplasmoniccouplingsynergisticallyimprovedlongrangesprbiosensorwithlowdetectionlimit
AT jiangjunfeng doubleantibodysandwichimmunoassayandplasmoniccouplingsynergisticallyimprovedlongrangesprbiosensorwithlowdetectionlimit
AT xutianhua doubleantibodysandwichimmunoassayandplasmoniccouplingsynergisticallyimprovedlongrangesprbiosensorwithlowdetectionlimit
AT wangshuang doubleantibodysandwichimmunoassayandplasmoniccouplingsynergisticallyimprovedlongrangesprbiosensorwithlowdetectionlimit
AT majinying doubleantibodysandwichimmunoassayandplasmoniccouplingsynergisticallyimprovedlongrangesprbiosensorwithlowdetectionlimit
AT zhangzhao doubleantibodysandwichimmunoassayandplasmoniccouplingsynergisticallyimprovedlongrangesprbiosensorwithlowdetectionlimit
AT zhangwenlin doubleantibodysandwichimmunoassayandplasmoniccouplingsynergisticallyimprovedlongrangesprbiosensorwithlowdetectionlimit
AT liutiegen doubleantibodysandwichimmunoassayandplasmoniccouplingsynergisticallyimprovedlongrangesprbiosensorwithlowdetectionlimit