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A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type

Mesenchymal stromal cells (MSCs) have shown a high potential for cartilage repair. Collagen-based scaffolds are used to deliver and retain cells at the site of cartilage damage. The aim of the work was a comparative analysis of the capacity of the MSCs from human adipose tissue to differentiate into...

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Autores principales: Sevastianov, Victor I., Basok, Yulia B., Kirsanova, Ludmila A., Grigoriev, Alexey M., Kirillova, Alexandra D., Nemets, Evgeniy A., Subbot, Anastasia M., Gautier, Sergey V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400656/
https://www.ncbi.nlm.nih.gov/pubmed/34440500
http://dx.doi.org/10.3390/life11080756
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author Sevastianov, Victor I.
Basok, Yulia B.
Kirsanova, Ludmila A.
Grigoriev, Alexey M.
Kirillova, Alexandra D.
Nemets, Evgeniy A.
Subbot, Anastasia M.
Gautier, Sergey V.
author_facet Sevastianov, Victor I.
Basok, Yulia B.
Kirsanova, Ludmila A.
Grigoriev, Alexey M.
Kirillova, Alexandra D.
Nemets, Evgeniy A.
Subbot, Anastasia M.
Gautier, Sergey V.
author_sort Sevastianov, Victor I.
collection PubMed
description Mesenchymal stromal cells (MSCs) have shown a high potential for cartilage repair. Collagen-based scaffolds are used to deliver and retain cells at the site of cartilage damage. The aim of the work was a comparative analysis of the capacity of the MSCs from human adipose tissue to differentiate into chondrocytes in vitro and to stimulate the regeneration of articular cartilage in an experimental model of rabbit knee osteoarthrosis when cultured on microheterogenic collagen-based hydrogel (MCH) and the microparticles of decellularized porcine articular cartilage (DPC). The morphology of samples was evaluated using scanning electron microscopy and histological staining methods. On the surface of the DPC, the cells were distributed more uniformly than on the MCH surface. On day 28, the cells cultured on the DPC produced glycosaminoglycans more intensely compared to the MCH with the synthesis of collagen type II. However, in the experimental model of osteoarthrosis, the stimulation of the cartilage regeneration was more effective when the MSCs were administered to the MCH carrier. The present study demonstrates the way to regulate the action of the MSCs in the area of cartilage regeneration: the MCH is more conducive to stimulating cartilage repair by the MSCs, while the DPC is an inducer for a formation of a cartilage-like tissue by the MSCs in vitro.
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spelling pubmed-84006562021-08-29 A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type Sevastianov, Victor I. Basok, Yulia B. Kirsanova, Ludmila A. Grigoriev, Alexey M. Kirillova, Alexandra D. Nemets, Evgeniy A. Subbot, Anastasia M. Gautier, Sergey V. Life (Basel) Article Mesenchymal stromal cells (MSCs) have shown a high potential for cartilage repair. Collagen-based scaffolds are used to deliver and retain cells at the site of cartilage damage. The aim of the work was a comparative analysis of the capacity of the MSCs from human adipose tissue to differentiate into chondrocytes in vitro and to stimulate the regeneration of articular cartilage in an experimental model of rabbit knee osteoarthrosis when cultured on microheterogenic collagen-based hydrogel (MCH) and the microparticles of decellularized porcine articular cartilage (DPC). The morphology of samples was evaluated using scanning electron microscopy and histological staining methods. On the surface of the DPC, the cells were distributed more uniformly than on the MCH surface. On day 28, the cells cultured on the DPC produced glycosaminoglycans more intensely compared to the MCH with the synthesis of collagen type II. However, in the experimental model of osteoarthrosis, the stimulation of the cartilage regeneration was more effective when the MSCs were administered to the MCH carrier. The present study demonstrates the way to regulate the action of the MSCs in the area of cartilage regeneration: the MCH is more conducive to stimulating cartilage repair by the MSCs, while the DPC is an inducer for a formation of a cartilage-like tissue by the MSCs in vitro. MDPI 2021-07-27 /pmc/articles/PMC8400656/ /pubmed/34440500 http://dx.doi.org/10.3390/life11080756 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sevastianov, Victor I.
Basok, Yulia B.
Kirsanova, Ludmila A.
Grigoriev, Alexey M.
Kirillova, Alexandra D.
Nemets, Evgeniy A.
Subbot, Anastasia M.
Gautier, Sergey V.
A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type
title A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type
title_full A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type
title_fullStr A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type
title_full_unstemmed A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type
title_short A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type
title_sort comparison of the capacity of mesenchymal stromal cells for cartilage regeneration depending on collagen-based injectable biomimetic scaffold type
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400656/
https://www.ncbi.nlm.nih.gov/pubmed/34440500
http://dx.doi.org/10.3390/life11080756
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