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New Insights into Blood Circulating Lymphocytes in Human Pneumocystis Pneumonia

The host lymphocyte response is decisive in Pneumocystis pneumonia (PCP) pathophysiology but little is known of the specific roles of lymphocyte subpopulations in this fungal infection. Peripheral NK, NKT, B, TCD4+ and TCD8+ subpopulations were compared by immunophenotyping between 20 patients diagn...

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Autores principales: Charpentier, Eléna, Marques, Catherine, Ménard, Sandie, Chauvin, Pamela, Guemas, Emilie, Cottrel, Claire, Cassaing, Sophie, Fillaux, Judith, Valentin, Alexis, Blanchard, Nicolas, Berry, Antoine, Iriart, Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400826/
https://www.ncbi.nlm.nih.gov/pubmed/34436191
http://dx.doi.org/10.3390/jof7080652
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author Charpentier, Eléna
Marques, Catherine
Ménard, Sandie
Chauvin, Pamela
Guemas, Emilie
Cottrel, Claire
Cassaing, Sophie
Fillaux, Judith
Valentin, Alexis
Blanchard, Nicolas
Berry, Antoine
Iriart, Xavier
author_facet Charpentier, Eléna
Marques, Catherine
Ménard, Sandie
Chauvin, Pamela
Guemas, Emilie
Cottrel, Claire
Cassaing, Sophie
Fillaux, Judith
Valentin, Alexis
Blanchard, Nicolas
Berry, Antoine
Iriart, Xavier
author_sort Charpentier, Eléna
collection PubMed
description The host lymphocyte response is decisive in Pneumocystis pneumonia (PCP) pathophysiology but little is known of the specific roles of lymphocyte subpopulations in this fungal infection. Peripheral NK, NKT, B, TCD4+ and TCD8+ subpopulations were compared by immunophenotyping between 20 patients diagnosed with PCP (PCP(+)] and 20 uninfected immunosuppressed patients (PCP(−)). Among PCP(+) subjects, the lymphocyte populations were also compared between surviving and deceased patients. Low B cell count (<40 cells/µL) was more frequent in PCP(+) than in PCP(−) patients (p = 0.03), while there was no difference for the TCD4 count. Among the PCP(+) group, the 7 deceased patients had lower Th1 (p = 0.02) and Tc1 (p = 0.03) populations, higher Th2 response (p = 0.03), higher effector TCD8 (p < 0.01), lower central memory TCD8 (p = 0.04) and reduced NK cells (p = 0.02) compared with the 13 survivors. Th1/Th2 ratio < 17, CD8 Tc1 < 44%, effector TCD8 < 25%, central memory TCD8 < 4%, NK cells < 50 cells/µL and total lymphocytes < 0.75 G/L were associated with a higher risk of mortality (p = 0.003, p = 0.007, p = 0.0007, p = 0.004, p = 0.02 and p = 0.019, respectively). The traditional analysis of TCD4 and TCD8 populations may be insufficient in the context of PCP. It could be completed by using B cells to predict the risk of PCP, and by using lymphocyte subpopulations or total lymphocyte count, which are easy to obtain in all health care facilities, to evaluate PCP prognosis.
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spelling pubmed-84008262021-08-29 New Insights into Blood Circulating Lymphocytes in Human Pneumocystis Pneumonia Charpentier, Eléna Marques, Catherine Ménard, Sandie Chauvin, Pamela Guemas, Emilie Cottrel, Claire Cassaing, Sophie Fillaux, Judith Valentin, Alexis Blanchard, Nicolas Berry, Antoine Iriart, Xavier J Fungi (Basel) Article The host lymphocyte response is decisive in Pneumocystis pneumonia (PCP) pathophysiology but little is known of the specific roles of lymphocyte subpopulations in this fungal infection. Peripheral NK, NKT, B, TCD4+ and TCD8+ subpopulations were compared by immunophenotyping between 20 patients diagnosed with PCP (PCP(+)] and 20 uninfected immunosuppressed patients (PCP(−)). Among PCP(+) subjects, the lymphocyte populations were also compared between surviving and deceased patients. Low B cell count (<40 cells/µL) was more frequent in PCP(+) than in PCP(−) patients (p = 0.03), while there was no difference for the TCD4 count. Among the PCP(+) group, the 7 deceased patients had lower Th1 (p = 0.02) and Tc1 (p = 0.03) populations, higher Th2 response (p = 0.03), higher effector TCD8 (p < 0.01), lower central memory TCD8 (p = 0.04) and reduced NK cells (p = 0.02) compared with the 13 survivors. Th1/Th2 ratio < 17, CD8 Tc1 < 44%, effector TCD8 < 25%, central memory TCD8 < 4%, NK cells < 50 cells/µL and total lymphocytes < 0.75 G/L were associated with a higher risk of mortality (p = 0.003, p = 0.007, p = 0.0007, p = 0.004, p = 0.02 and p = 0.019, respectively). The traditional analysis of TCD4 and TCD8 populations may be insufficient in the context of PCP. It could be completed by using B cells to predict the risk of PCP, and by using lymphocyte subpopulations or total lymphocyte count, which are easy to obtain in all health care facilities, to evaluate PCP prognosis. MDPI 2021-08-11 /pmc/articles/PMC8400826/ /pubmed/34436191 http://dx.doi.org/10.3390/jof7080652 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Charpentier, Eléna
Marques, Catherine
Ménard, Sandie
Chauvin, Pamela
Guemas, Emilie
Cottrel, Claire
Cassaing, Sophie
Fillaux, Judith
Valentin, Alexis
Blanchard, Nicolas
Berry, Antoine
Iriart, Xavier
New Insights into Blood Circulating Lymphocytes in Human Pneumocystis Pneumonia
title New Insights into Blood Circulating Lymphocytes in Human Pneumocystis Pneumonia
title_full New Insights into Blood Circulating Lymphocytes in Human Pneumocystis Pneumonia
title_fullStr New Insights into Blood Circulating Lymphocytes in Human Pneumocystis Pneumonia
title_full_unstemmed New Insights into Blood Circulating Lymphocytes in Human Pneumocystis Pneumonia
title_short New Insights into Blood Circulating Lymphocytes in Human Pneumocystis Pneumonia
title_sort new insights into blood circulating lymphocytes in human pneumocystis pneumonia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400826/
https://www.ncbi.nlm.nih.gov/pubmed/34436191
http://dx.doi.org/10.3390/jof7080652
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