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Phenolics-Rich Extracts of Dietary Plants as Regulators of Fructose Uptake in Caco-2 Cells via GLUT5 Involvement

The latest data link the chronic consumption of large amounts of fructose present in food with the generation of hypertension and disturbances in carbohydrate and lipid metabolism, which promote the development of obesity, non-alcoholic fatty liver disease, insulin resistance, and type 2 diabetes. T...

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Autores principales: Zakłos-Szyda, Małgorzata, Pietrzyk, Nina, Kowalska-Baron, Agnieszka, Nowak, Adriana, Chałaśkiewicz, Katarzyna, Ratajewski, Marcin, Budryn, Grażyna, Koziołkiewicz, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401051/
https://www.ncbi.nlm.nih.gov/pubmed/34443333
http://dx.doi.org/10.3390/molecules26164745
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author Zakłos-Szyda, Małgorzata
Pietrzyk, Nina
Kowalska-Baron, Agnieszka
Nowak, Adriana
Chałaśkiewicz, Katarzyna
Ratajewski, Marcin
Budryn, Grażyna
Koziołkiewicz, Maria
author_facet Zakłos-Szyda, Małgorzata
Pietrzyk, Nina
Kowalska-Baron, Agnieszka
Nowak, Adriana
Chałaśkiewicz, Katarzyna
Ratajewski, Marcin
Budryn, Grażyna
Koziołkiewicz, Maria
author_sort Zakłos-Szyda, Małgorzata
collection PubMed
description The latest data link the chronic consumption of large amounts of fructose present in food with the generation of hypertension and disturbances in carbohydrate and lipid metabolism, which promote the development of obesity, non-alcoholic fatty liver disease, insulin resistance, and type 2 diabetes. This effect is possible after fructose is absorbed by the small intestine cells and, to a lesser extent, by hepatocytes. Fructose transport is dependent on proteins from the family of glucose transporters (GLUTs), among which GLUT5 selectively absorbs fructose from the intestine. In this study, we examined the effect of four phenolic-rich extracts obtained from A. graveolens, B. juncea, and M. chamomilla on fructose uptake by Caco-2 cells. Extracts from B. juncea and M. chamomilla most effectively reduced fluorescent fructose analogue (NBDF) accumulation in Caco-2, as well as downregulated GLUT5 protein levels. These preparations were able to decrease the mRNA level of genes encoding transcription factors regulating GLUT5 expression-thioredoxin-interacting protein (TXNIP) and carbohydrate-responsive element-binding protein (ChREBP). Active extracts contained large amounts of apigenin and flavonols. The molecular docking simulation suggested that some of identified phenolic constituents can play an important role in the inhibition of GLUT5-mediated fructose transport.
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spelling pubmed-84010512021-08-29 Phenolics-Rich Extracts of Dietary Plants as Regulators of Fructose Uptake in Caco-2 Cells via GLUT5 Involvement Zakłos-Szyda, Małgorzata Pietrzyk, Nina Kowalska-Baron, Agnieszka Nowak, Adriana Chałaśkiewicz, Katarzyna Ratajewski, Marcin Budryn, Grażyna Koziołkiewicz, Maria Molecules Article The latest data link the chronic consumption of large amounts of fructose present in food with the generation of hypertension and disturbances in carbohydrate and lipid metabolism, which promote the development of obesity, non-alcoholic fatty liver disease, insulin resistance, and type 2 diabetes. This effect is possible after fructose is absorbed by the small intestine cells and, to a lesser extent, by hepatocytes. Fructose transport is dependent on proteins from the family of glucose transporters (GLUTs), among which GLUT5 selectively absorbs fructose from the intestine. In this study, we examined the effect of four phenolic-rich extracts obtained from A. graveolens, B. juncea, and M. chamomilla on fructose uptake by Caco-2 cells. Extracts from B. juncea and M. chamomilla most effectively reduced fluorescent fructose analogue (NBDF) accumulation in Caco-2, as well as downregulated GLUT5 protein levels. These preparations were able to decrease the mRNA level of genes encoding transcription factors regulating GLUT5 expression-thioredoxin-interacting protein (TXNIP) and carbohydrate-responsive element-binding protein (ChREBP). Active extracts contained large amounts of apigenin and flavonols. The molecular docking simulation suggested that some of identified phenolic constituents can play an important role in the inhibition of GLUT5-mediated fructose transport. MDPI 2021-08-05 /pmc/articles/PMC8401051/ /pubmed/34443333 http://dx.doi.org/10.3390/molecules26164745 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zakłos-Szyda, Małgorzata
Pietrzyk, Nina
Kowalska-Baron, Agnieszka
Nowak, Adriana
Chałaśkiewicz, Katarzyna
Ratajewski, Marcin
Budryn, Grażyna
Koziołkiewicz, Maria
Phenolics-Rich Extracts of Dietary Plants as Regulators of Fructose Uptake in Caco-2 Cells via GLUT5 Involvement
title Phenolics-Rich Extracts of Dietary Plants as Regulators of Fructose Uptake in Caco-2 Cells via GLUT5 Involvement
title_full Phenolics-Rich Extracts of Dietary Plants as Regulators of Fructose Uptake in Caco-2 Cells via GLUT5 Involvement
title_fullStr Phenolics-Rich Extracts of Dietary Plants as Regulators of Fructose Uptake in Caco-2 Cells via GLUT5 Involvement
title_full_unstemmed Phenolics-Rich Extracts of Dietary Plants as Regulators of Fructose Uptake in Caco-2 Cells via GLUT5 Involvement
title_short Phenolics-Rich Extracts of Dietary Plants as Regulators of Fructose Uptake in Caco-2 Cells via GLUT5 Involvement
title_sort phenolics-rich extracts of dietary plants as regulators of fructose uptake in caco-2 cells via glut5 involvement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401051/
https://www.ncbi.nlm.nih.gov/pubmed/34443333
http://dx.doi.org/10.3390/molecules26164745
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