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Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells

With the emergence of coronavirus disease-2019, researchers have gained interest in the therapeutic efficacy of mesenchymal stem/stromal cells (MSCs) in acute respiratory distress syndrome; however, the mechanisms of the therapeutic effects of MSCs are unclear. We have previously reported that adipo...

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Autores principales: Ishii, Mitsutoshi, Tsuchiya, Tomoshi, Doi, Ryoichiro, Morofuji, Yoichi, Fujimoto, Takashi, Muto, Hideki, Suematsu, Takashi, Mori, Ryoichi, Matsumoto, Keitaro, Miyazaki, Takuro, Tomoshige, Koichi, Watanabe, Hironosuke, Iwatake, Mayumi, Nagayasu, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401152/
https://www.ncbi.nlm.nih.gov/pubmed/34452225
http://dx.doi.org/10.3390/pharmaceutics13081264
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author Ishii, Mitsutoshi
Tsuchiya, Tomoshi
Doi, Ryoichiro
Morofuji, Yoichi
Fujimoto, Takashi
Muto, Hideki
Suematsu, Takashi
Mori, Ryoichi
Matsumoto, Keitaro
Miyazaki, Takuro
Tomoshige, Koichi
Watanabe, Hironosuke
Iwatake, Mayumi
Nagayasu, Takeshi
author_facet Ishii, Mitsutoshi
Tsuchiya, Tomoshi
Doi, Ryoichiro
Morofuji, Yoichi
Fujimoto, Takashi
Muto, Hideki
Suematsu, Takashi
Mori, Ryoichi
Matsumoto, Keitaro
Miyazaki, Takuro
Tomoshige, Koichi
Watanabe, Hironosuke
Iwatake, Mayumi
Nagayasu, Takeshi
author_sort Ishii, Mitsutoshi
collection PubMed
description With the emergence of coronavirus disease-2019, researchers have gained interest in the therapeutic efficacy of mesenchymal stem/stromal cells (MSCs) in acute respiratory distress syndrome; however, the mechanisms of the therapeutic effects of MSCs are unclear. We have previously reported that adipose-derived MSCs (AD-MSCs) strengthen the barrier function of the pulmonary vessels in scaffold-based bioengineered rat lungs. In this study, we evaluated whether AD-MSCs could enhance the intercellular barrier function of lung epithelial cells in vitro using a transwell coculture system. Transepithelial electrical resistance (TEER) measurements revealed that the peak TEER value was significantly higher in the AD-MSC coculture group than in the AD-MSC non-coculture group. Similarly, the permeability coefficient was significantly decreased in the AD-MSC coculture group compared to that in the AD-MSC non-coculture group. Immunostaining of insert membranes showed that zonula occuldens-1 expression was significantly high at cell junctions in the AD-MSC coculture group. Moreover, cell junction-related gene profiling showed that the expression of some claudin genes, including claudin-4, was upregulated in the AD-MSC coculture group. Taken together, these results showed that AD-MSCs enhanced the barrier function between lung epithelial cells, suggesting that both direct adhesion and indirect paracrine effects strengthened the barrier function of lung alveolar epithelium in vitro.
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spelling pubmed-84011522021-08-29 Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells Ishii, Mitsutoshi Tsuchiya, Tomoshi Doi, Ryoichiro Morofuji, Yoichi Fujimoto, Takashi Muto, Hideki Suematsu, Takashi Mori, Ryoichi Matsumoto, Keitaro Miyazaki, Takuro Tomoshige, Koichi Watanabe, Hironosuke Iwatake, Mayumi Nagayasu, Takeshi Pharmaceutics Article With the emergence of coronavirus disease-2019, researchers have gained interest in the therapeutic efficacy of mesenchymal stem/stromal cells (MSCs) in acute respiratory distress syndrome; however, the mechanisms of the therapeutic effects of MSCs are unclear. We have previously reported that adipose-derived MSCs (AD-MSCs) strengthen the barrier function of the pulmonary vessels in scaffold-based bioengineered rat lungs. In this study, we evaluated whether AD-MSCs could enhance the intercellular barrier function of lung epithelial cells in vitro using a transwell coculture system. Transepithelial electrical resistance (TEER) measurements revealed that the peak TEER value was significantly higher in the AD-MSC coculture group than in the AD-MSC non-coculture group. Similarly, the permeability coefficient was significantly decreased in the AD-MSC coculture group compared to that in the AD-MSC non-coculture group. Immunostaining of insert membranes showed that zonula occuldens-1 expression was significantly high at cell junctions in the AD-MSC coculture group. Moreover, cell junction-related gene profiling showed that the expression of some claudin genes, including claudin-4, was upregulated in the AD-MSC coculture group. Taken together, these results showed that AD-MSCs enhanced the barrier function between lung epithelial cells, suggesting that both direct adhesion and indirect paracrine effects strengthened the barrier function of lung alveolar epithelium in vitro. MDPI 2021-08-16 /pmc/articles/PMC8401152/ /pubmed/34452225 http://dx.doi.org/10.3390/pharmaceutics13081264 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ishii, Mitsutoshi
Tsuchiya, Tomoshi
Doi, Ryoichiro
Morofuji, Yoichi
Fujimoto, Takashi
Muto, Hideki
Suematsu, Takashi
Mori, Ryoichi
Matsumoto, Keitaro
Miyazaki, Takuro
Tomoshige, Koichi
Watanabe, Hironosuke
Iwatake, Mayumi
Nagayasu, Takeshi
Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells
title Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells
title_full Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells
title_fullStr Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells
title_full_unstemmed Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells
title_short Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells
title_sort increased in vitro intercellular barrier function of lung epithelial cells using adipose-derived mesenchymal stem/stromal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401152/
https://www.ncbi.nlm.nih.gov/pubmed/34452225
http://dx.doi.org/10.3390/pharmaceutics13081264
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