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Incorporation of Arginine to Commercial Orthodontic Light-Cured Resin Cements—Physical, Adhesive, and Antibacterial Properties

(1) Background: The amino acid arginine is now receiving great attention due to its potential anti-caries benefits. The purpose of this in vitro study was to evaluate the shear bond strength (SBS), ultimate tensile strength (UTS), and antimicrobial potential (CFU) of two arginine-containing orthodon...

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Autores principales: Geraldeli, Saulo, Maia Carvalho, Lucas de Almeida, de Souza Araújo, Isaac Jordão, Guarda, Maurício Bottene, Nascimento, Marcelle M., Bertolo, Marcus Vinícius Loureiro, Di Nizo, Paolo Túlio, Sinhoreti, Mário Alexandre Coelho, McCarlie, V. Wallace
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401166/
https://www.ncbi.nlm.nih.gov/pubmed/34442914
http://dx.doi.org/10.3390/ma14164391
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author Geraldeli, Saulo
Maia Carvalho, Lucas de Almeida
de Souza Araújo, Isaac Jordão
Guarda, Maurício Bottene
Nascimento, Marcelle M.
Bertolo, Marcus Vinícius Loureiro
Di Nizo, Paolo Túlio
Sinhoreti, Mário Alexandre Coelho
McCarlie, V. Wallace
author_facet Geraldeli, Saulo
Maia Carvalho, Lucas de Almeida
de Souza Araújo, Isaac Jordão
Guarda, Maurício Bottene
Nascimento, Marcelle M.
Bertolo, Marcus Vinícius Loureiro
Di Nizo, Paolo Túlio
Sinhoreti, Mário Alexandre Coelho
McCarlie, V. Wallace
author_sort Geraldeli, Saulo
collection PubMed
description (1) Background: The amino acid arginine is now receiving great attention due to its potential anti-caries benefits. The purpose of this in vitro study was to evaluate the shear bond strength (SBS), ultimate tensile strength (UTS), and antimicrobial potential (CFU) of two arginine-containing orthodontic resin cements. (2) Methods: Forty bovine incisors were separated into four groups (n = 10): Orthocem, Orthocem + arginine (2.5 wt%), Transbond XT, and Transbond XT + arginine (2.5 wt%). The brackets were fixed to the flat surface of the enamel, and after 24 h the SBS was evaluated using the universal testing machine (Instron). For the UTS test, hourglass samples (n = 10) were made and tested in a mini-testing machine (OM-100, Odeme). For the antibacterial test (colony forming unit-CFU), six cement discs from each group were made and exposed to Streptococcus mutans UA159 biofilm for 7 days. The microbiological experiment was performed by serial and triplicate dilutions. The data from each test were statistically analyzed using a two-way ANOVA, followed by Tukey’s test (α = 0.05). (3) Results: The enamel SBS mean values of Transbond XT were statistically higher than those of Orthocem, both with and without arginine (p = 0.02033). There was no significant difference in the SBS mean values between the orthodontic resin cements, either with or without arginine (p = 0.29869). The UTS of the Transbond XT was statistically higher than the Orthocem, but the addition of arginine at 2.5 wt% did not influence the UTS for either resin cement. The Orthocem + arginine orthodontic resin cement was able to significantly reduce S. mutans growth, but no difference was observed for the Transbond XT (p = 0.03439). (4) Conclusion: The incorporation of arginine to commercial orthodontic resin cements may be an efficient preventive strategy to reduce bacterial growth without compromising their adhesive and mechanical properties.
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spelling pubmed-84011662021-08-29 Incorporation of Arginine to Commercial Orthodontic Light-Cured Resin Cements—Physical, Adhesive, and Antibacterial Properties Geraldeli, Saulo Maia Carvalho, Lucas de Almeida de Souza Araújo, Isaac Jordão Guarda, Maurício Bottene Nascimento, Marcelle M. Bertolo, Marcus Vinícius Loureiro Di Nizo, Paolo Túlio Sinhoreti, Mário Alexandre Coelho McCarlie, V. Wallace Materials (Basel) Article (1) Background: The amino acid arginine is now receiving great attention due to its potential anti-caries benefits. The purpose of this in vitro study was to evaluate the shear bond strength (SBS), ultimate tensile strength (UTS), and antimicrobial potential (CFU) of two arginine-containing orthodontic resin cements. (2) Methods: Forty bovine incisors were separated into four groups (n = 10): Orthocem, Orthocem + arginine (2.5 wt%), Transbond XT, and Transbond XT + arginine (2.5 wt%). The brackets were fixed to the flat surface of the enamel, and after 24 h the SBS was evaluated using the universal testing machine (Instron). For the UTS test, hourglass samples (n = 10) were made and tested in a mini-testing machine (OM-100, Odeme). For the antibacterial test (colony forming unit-CFU), six cement discs from each group were made and exposed to Streptococcus mutans UA159 biofilm for 7 days. The microbiological experiment was performed by serial and triplicate dilutions. The data from each test were statistically analyzed using a two-way ANOVA, followed by Tukey’s test (α = 0.05). (3) Results: The enamel SBS mean values of Transbond XT were statistically higher than those of Orthocem, both with and without arginine (p = 0.02033). There was no significant difference in the SBS mean values between the orthodontic resin cements, either with or without arginine (p = 0.29869). The UTS of the Transbond XT was statistically higher than the Orthocem, but the addition of arginine at 2.5 wt% did not influence the UTS for either resin cement. The Orthocem + arginine orthodontic resin cement was able to significantly reduce S. mutans growth, but no difference was observed for the Transbond XT (p = 0.03439). (4) Conclusion: The incorporation of arginine to commercial orthodontic resin cements may be an efficient preventive strategy to reduce bacterial growth without compromising their adhesive and mechanical properties. MDPI 2021-08-05 /pmc/articles/PMC8401166/ /pubmed/34442914 http://dx.doi.org/10.3390/ma14164391 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Geraldeli, Saulo
Maia Carvalho, Lucas de Almeida
de Souza Araújo, Isaac Jordão
Guarda, Maurício Bottene
Nascimento, Marcelle M.
Bertolo, Marcus Vinícius Loureiro
Di Nizo, Paolo Túlio
Sinhoreti, Mário Alexandre Coelho
McCarlie, V. Wallace
Incorporation of Arginine to Commercial Orthodontic Light-Cured Resin Cements—Physical, Adhesive, and Antibacterial Properties
title Incorporation of Arginine to Commercial Orthodontic Light-Cured Resin Cements—Physical, Adhesive, and Antibacterial Properties
title_full Incorporation of Arginine to Commercial Orthodontic Light-Cured Resin Cements—Physical, Adhesive, and Antibacterial Properties
title_fullStr Incorporation of Arginine to Commercial Orthodontic Light-Cured Resin Cements—Physical, Adhesive, and Antibacterial Properties
title_full_unstemmed Incorporation of Arginine to Commercial Orthodontic Light-Cured Resin Cements—Physical, Adhesive, and Antibacterial Properties
title_short Incorporation of Arginine to Commercial Orthodontic Light-Cured Resin Cements—Physical, Adhesive, and Antibacterial Properties
title_sort incorporation of arginine to commercial orthodontic light-cured resin cements—physical, adhesive, and antibacterial properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401166/
https://www.ncbi.nlm.nih.gov/pubmed/34442914
http://dx.doi.org/10.3390/ma14164391
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