Cargando…

Cranberry (Vaccinium macrocarpon) Extract Impairs Nairovirus Infection by Inhibiting the Attachment to Target Cells

Hazara virus (HAZV) belongs to the Nairoviridae family and is included in the same serogroup of the Crimean-Congo hemorrhagic fever virus (CCHFV). CCHFV is the most widespread tick-borne arbovirus. It is responsible for a serious hemorrhagic disease, for which specific and effective treatment and pr...

Descripción completa

Detalles Bibliográficos
Autores principales: Mirandola, Mattia, Salvati, Maria Vittoria, Rodigari, Carola, Appelberg, K. Sofia, Mirazimi, Ali, Maffei, Massimo E., Gribaudo, Giorgio, Salata, Cristiano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401317/
https://www.ncbi.nlm.nih.gov/pubmed/34451488
http://dx.doi.org/10.3390/pathogens10081025
_version_ 1783745522237964288
author Mirandola, Mattia
Salvati, Maria Vittoria
Rodigari, Carola
Appelberg, K. Sofia
Mirazimi, Ali
Maffei, Massimo E.
Gribaudo, Giorgio
Salata, Cristiano
author_facet Mirandola, Mattia
Salvati, Maria Vittoria
Rodigari, Carola
Appelberg, K. Sofia
Mirazimi, Ali
Maffei, Massimo E.
Gribaudo, Giorgio
Salata, Cristiano
author_sort Mirandola, Mattia
collection PubMed
description Hazara virus (HAZV) belongs to the Nairoviridae family and is included in the same serogroup of the Crimean-Congo hemorrhagic fever virus (CCHFV). CCHFV is the most widespread tick-borne arbovirus. It is responsible for a serious hemorrhagic disease, for which specific and effective treatment and preventive systems are missing. Bioactive compounds derived from several natural products may provide a natural source of broad-spectrum antiviral agents, characterized by good tolerability and minimal side effects. Previous in vitro studies have shown that a cranberry (Vaccinium macrocarpon Ait.) extract containing a high content of A-type proanthocyanidins (PAC-A) inhibits the replication of herpes simplex and influenza viruses by hampering their attachment to target cells. Given the broad-spectrum antimicrobial activity of polyphenols and the urgency to develop therapies for the treatment of CCHF, we investigated the antiviral activity of cranberry extract against HAZV, a surrogate nairovirus model of CCHFV that can be handled in Level 2 Biosafety Laboratories (BSL-2). The results indicate that the cranberry extract exerts an antiviral activity against HAZV by targeting early stages of the viral replication cycle, including the initial adsorption to target cells. Although the details of the molecular mechanism of action remain to be clarified, the cranberry extract exerts a virucidal effect through a direct interaction with HAZV particles that leads to the subsequent impairment of virus attachment to cell-surface receptors. Finally, the antiviral activity of the cranberry extract was also confirmed for CCHFV. As a whole, the evidence obtained suggests that cranberry extract is a valuable candidate to be considered for the development of therapeutic strategies for CCHFV infections.
format Online
Article
Text
id pubmed-8401317
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84013172021-08-29 Cranberry (Vaccinium macrocarpon) Extract Impairs Nairovirus Infection by Inhibiting the Attachment to Target Cells Mirandola, Mattia Salvati, Maria Vittoria Rodigari, Carola Appelberg, K. Sofia Mirazimi, Ali Maffei, Massimo E. Gribaudo, Giorgio Salata, Cristiano Pathogens Article Hazara virus (HAZV) belongs to the Nairoviridae family and is included in the same serogroup of the Crimean-Congo hemorrhagic fever virus (CCHFV). CCHFV is the most widespread tick-borne arbovirus. It is responsible for a serious hemorrhagic disease, for which specific and effective treatment and preventive systems are missing. Bioactive compounds derived from several natural products may provide a natural source of broad-spectrum antiviral agents, characterized by good tolerability and minimal side effects. Previous in vitro studies have shown that a cranberry (Vaccinium macrocarpon Ait.) extract containing a high content of A-type proanthocyanidins (PAC-A) inhibits the replication of herpes simplex and influenza viruses by hampering their attachment to target cells. Given the broad-spectrum antimicrobial activity of polyphenols and the urgency to develop therapies for the treatment of CCHF, we investigated the antiviral activity of cranberry extract against HAZV, a surrogate nairovirus model of CCHFV that can be handled in Level 2 Biosafety Laboratories (BSL-2). The results indicate that the cranberry extract exerts an antiviral activity against HAZV by targeting early stages of the viral replication cycle, including the initial adsorption to target cells. Although the details of the molecular mechanism of action remain to be clarified, the cranberry extract exerts a virucidal effect through a direct interaction with HAZV particles that leads to the subsequent impairment of virus attachment to cell-surface receptors. Finally, the antiviral activity of the cranberry extract was also confirmed for CCHFV. As a whole, the evidence obtained suggests that cranberry extract is a valuable candidate to be considered for the development of therapeutic strategies for CCHFV infections. MDPI 2021-08-13 /pmc/articles/PMC8401317/ /pubmed/34451488 http://dx.doi.org/10.3390/pathogens10081025 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mirandola, Mattia
Salvati, Maria Vittoria
Rodigari, Carola
Appelberg, K. Sofia
Mirazimi, Ali
Maffei, Massimo E.
Gribaudo, Giorgio
Salata, Cristiano
Cranberry (Vaccinium macrocarpon) Extract Impairs Nairovirus Infection by Inhibiting the Attachment to Target Cells
title Cranberry (Vaccinium macrocarpon) Extract Impairs Nairovirus Infection by Inhibiting the Attachment to Target Cells
title_full Cranberry (Vaccinium macrocarpon) Extract Impairs Nairovirus Infection by Inhibiting the Attachment to Target Cells
title_fullStr Cranberry (Vaccinium macrocarpon) Extract Impairs Nairovirus Infection by Inhibiting the Attachment to Target Cells
title_full_unstemmed Cranberry (Vaccinium macrocarpon) Extract Impairs Nairovirus Infection by Inhibiting the Attachment to Target Cells
title_short Cranberry (Vaccinium macrocarpon) Extract Impairs Nairovirus Infection by Inhibiting the Attachment to Target Cells
title_sort cranberry (vaccinium macrocarpon) extract impairs nairovirus infection by inhibiting the attachment to target cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401317/
https://www.ncbi.nlm.nih.gov/pubmed/34451488
http://dx.doi.org/10.3390/pathogens10081025
work_keys_str_mv AT mirandolamattia cranberryvacciniummacrocarponextractimpairsnairovirusinfectionbyinhibitingtheattachmenttotargetcells
AT salvatimariavittoria cranberryvacciniummacrocarponextractimpairsnairovirusinfectionbyinhibitingtheattachmenttotargetcells
AT rodigaricarola cranberryvacciniummacrocarponextractimpairsnairovirusinfectionbyinhibitingtheattachmenttotargetcells
AT appelbergksofia cranberryvacciniummacrocarponextractimpairsnairovirusinfectionbyinhibitingtheattachmenttotargetcells
AT mirazimiali cranberryvacciniummacrocarponextractimpairsnairovirusinfectionbyinhibitingtheattachmenttotargetcells
AT maffeimassimoe cranberryvacciniummacrocarponextractimpairsnairovirusinfectionbyinhibitingtheattachmenttotargetcells
AT gribaudogiorgio cranberryvacciniummacrocarponextractimpairsnairovirusinfectionbyinhibitingtheattachmenttotargetcells
AT salatacristiano cranberryvacciniummacrocarponextractimpairsnairovirusinfectionbyinhibitingtheattachmenttotargetcells