(1)H, (13)C and (15)N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2

The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7–33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is pres...

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Detalles Bibliográficos
Autores principales: Richter, Christian, Hohmann, Katharina F., Toews, Sabrina, Mathieu, Daniel, Altincekic, Nadide, Bains, Jasleen Kaur, Binas, Oliver, Ceylan, Betül, Duchardt-Ferner, Elke, Ferner, Jan, Fürtig, Boris, Grün, J. Tassilo, Hengesbach, Martin, Hymon, Daniel, Jonker, Hendrik R. A., Knezic, Bozana, Korn, Sophie M., Landgraf, Tom, Löhr, Frank, Peter, Stephen A., Pyper, Dennis J., Qureshi, Nusrat S., Schlundt, Andreas, Schnieders, Robbin, Stirnal, Elke, Sudakov, Alexey, Vögele, Jennifer, Weigand, Julia E., Wirmer-Bartoschek, Julia, Witt, Kerstin, Wöhnert, Jens, Schwalbe, Harald, Wacker, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401371/
https://www.ncbi.nlm.nih.gov/pubmed/34453696
http://dx.doi.org/10.1007/s12104-021-10047-2
Descripción
Sumario:The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7–33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the (1)H, (13)C and (15)N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12104-021-10047-2.

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