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(1)H, (13)C and (15)N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2
The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7–33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is pres...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Netherlands
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401371/ https://www.ncbi.nlm.nih.gov/pubmed/34453696 http://dx.doi.org/10.1007/s12104-021-10047-2 |
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| author | Richter, Christian Hohmann, Katharina F. Toews, Sabrina Mathieu, Daniel Altincekic, Nadide Bains, Jasleen Kaur Binas, Oliver Ceylan, Betül Duchardt-Ferner, Elke Ferner, Jan Fürtig, Boris Grün, J. Tassilo Hengesbach, Martin Hymon, Daniel Jonker, Hendrik R. A. Knezic, Bozana Korn, Sophie M. Landgraf, Tom Löhr, Frank Peter, Stephen A. Pyper, Dennis J. Qureshi, Nusrat S. Schlundt, Andreas Schnieders, Robbin Stirnal, Elke Sudakov, Alexey Vögele, Jennifer Weigand, Julia E. Wirmer-Bartoschek, Julia Witt, Kerstin Wöhnert, Jens Schwalbe, Harald Wacker, Anna |
| author_facet | Richter, Christian Hohmann, Katharina F. Toews, Sabrina Mathieu, Daniel Altincekic, Nadide Bains, Jasleen Kaur Binas, Oliver Ceylan, Betül Duchardt-Ferner, Elke Ferner, Jan Fürtig, Boris Grün, J. Tassilo Hengesbach, Martin Hymon, Daniel Jonker, Hendrik R. A. Knezic, Bozana Korn, Sophie M. Landgraf, Tom Löhr, Frank Peter, Stephen A. Pyper, Dennis J. Qureshi, Nusrat S. Schlundt, Andreas Schnieders, Robbin Stirnal, Elke Sudakov, Alexey Vögele, Jennifer Weigand, Julia E. Wirmer-Bartoschek, Julia Witt, Kerstin Wöhnert, Jens Schwalbe, Harald Wacker, Anna |
| author_sort | Richter, Christian |
| collection | PubMed |
| description | The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7–33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the (1)H, (13)C and (15)N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12104-021-10047-2. |
| format | Online Article Text |
| id | pubmed-8401371 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Springer Netherlands |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-84013712021-08-30 (1)H, (13)C and (15)N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2 Richter, Christian Hohmann, Katharina F. Toews, Sabrina Mathieu, Daniel Altincekic, Nadide Bains, Jasleen Kaur Binas, Oliver Ceylan, Betül Duchardt-Ferner, Elke Ferner, Jan Fürtig, Boris Grün, J. Tassilo Hengesbach, Martin Hymon, Daniel Jonker, Hendrik R. A. Knezic, Bozana Korn, Sophie M. Landgraf, Tom Löhr, Frank Peter, Stephen A. Pyper, Dennis J. Qureshi, Nusrat S. Schlundt, Andreas Schnieders, Robbin Stirnal, Elke Sudakov, Alexey Vögele, Jennifer Weigand, Julia E. Wirmer-Bartoschek, Julia Witt, Kerstin Wöhnert, Jens Schwalbe, Harald Wacker, Anna Biomol NMR Assign Article The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7–33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the (1)H, (13)C and (15)N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12104-021-10047-2. Springer Netherlands 2021-08-28 2021 /pmc/articles/PMC8401371/ /pubmed/34453696 http://dx.doi.org/10.1007/s12104-021-10047-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Article Richter, Christian Hohmann, Katharina F. Toews, Sabrina Mathieu, Daniel Altincekic, Nadide Bains, Jasleen Kaur Binas, Oliver Ceylan, Betül Duchardt-Ferner, Elke Ferner, Jan Fürtig, Boris Grün, J. Tassilo Hengesbach, Martin Hymon, Daniel Jonker, Hendrik R. A. Knezic, Bozana Korn, Sophie M. Landgraf, Tom Löhr, Frank Peter, Stephen A. Pyper, Dennis J. Qureshi, Nusrat S. Schlundt, Andreas Schnieders, Robbin Stirnal, Elke Sudakov, Alexey Vögele, Jennifer Weigand, Julia E. Wirmer-Bartoschek, Julia Witt, Kerstin Wöhnert, Jens Schwalbe, Harald Wacker, Anna (1)H, (13)C and (15)N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2 |
| title | (1)H, (13)C and (15)N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2 |
| title_full | (1)H, (13)C and (15)N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2 |
| title_fullStr | (1)H, (13)C and (15)N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2 |
| title_full_unstemmed | (1)H, (13)C and (15)N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2 |
| title_short | (1)H, (13)C and (15)N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2 |
| title_sort | (1)h, (13)c and (15)n assignment of stem-loop sl1 from the 5'-utr of sars-cov-2 |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401371/ https://www.ncbi.nlm.nih.gov/pubmed/34453696 http://dx.doi.org/10.1007/s12104-021-10047-2 |
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