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Agrobacterium-Mediated Genetic Transformation of Taiwanese Isolates of Lemna aequinoctialis

Duckweed (Lemna aequinoctialis) is one of the smallest flowering plants in the world. Due to its high reproduction rate and biomass, duckweeds are used as biofactors and feedstuff additives for livestock. It is also an ideal system for basic biological research and various practical applications. In...

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Autores principales: Wang, Kuang-Teng, Hong, Ming-Chang, Wu, Yu-Sheng, Wu, Tsung-Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401387/
https://www.ncbi.nlm.nih.gov/pubmed/34451621
http://dx.doi.org/10.3390/plants10081576
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author Wang, Kuang-Teng
Hong, Ming-Chang
Wu, Yu-Sheng
Wu, Tsung-Meng
author_facet Wang, Kuang-Teng
Hong, Ming-Chang
Wu, Yu-Sheng
Wu, Tsung-Meng
author_sort Wang, Kuang-Teng
collection PubMed
description Duckweed (Lemna aequinoctialis) is one of the smallest flowering plants in the world. Due to its high reproduction rate and biomass, duckweeds are used as biofactors and feedstuff additives for livestock. It is also an ideal system for basic biological research and various practical applications. In this study, we attempt to establish a micropropagation technique and Agrobacterium-mediated transformation in L. aequinoctialis. The plant-growth regulator type and concentration and Agrobacterium-mediated transformation were evaluated for their effects on duckweed callus induction, proliferation, regeneration, and gene transformation efficiency. Calli were successfully induced from 100% of explants on Murashige and Skoog (MS) medium containing 25.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.0 μM thidiazuron (TDZ). MS medium containing 4.5 μM 2,4-D and 2.0 μM TDZ supported the long-lasting growth of calli. Fronds regenerated from 100% of calli on Schenk and Hildebrandt (SH) medium containing 1.0 μM 6-benzyladenine (6-BA). We also determined that 200 μM acetosyringone in the cocultivation medium for 1 day in the dark was crucial for transformation efficiency (up to 3 ± 1%). Additionally, we propose that both techniques will facilitate efficient high-throughput genetic manipulation in Lemnaceae.
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spelling pubmed-84013872021-08-29 Agrobacterium-Mediated Genetic Transformation of Taiwanese Isolates of Lemna aequinoctialis Wang, Kuang-Teng Hong, Ming-Chang Wu, Yu-Sheng Wu, Tsung-Meng Plants (Basel) Article Duckweed (Lemna aequinoctialis) is one of the smallest flowering plants in the world. Due to its high reproduction rate and biomass, duckweeds are used as biofactors and feedstuff additives for livestock. It is also an ideal system for basic biological research and various practical applications. In this study, we attempt to establish a micropropagation technique and Agrobacterium-mediated transformation in L. aequinoctialis. The plant-growth regulator type and concentration and Agrobacterium-mediated transformation were evaluated for their effects on duckweed callus induction, proliferation, regeneration, and gene transformation efficiency. Calli were successfully induced from 100% of explants on Murashige and Skoog (MS) medium containing 25.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.0 μM thidiazuron (TDZ). MS medium containing 4.5 μM 2,4-D and 2.0 μM TDZ supported the long-lasting growth of calli. Fronds regenerated from 100% of calli on Schenk and Hildebrandt (SH) medium containing 1.0 μM 6-benzyladenine (6-BA). We also determined that 200 μM acetosyringone in the cocultivation medium for 1 day in the dark was crucial for transformation efficiency (up to 3 ± 1%). Additionally, we propose that both techniques will facilitate efficient high-throughput genetic manipulation in Lemnaceae. MDPI 2021-07-30 /pmc/articles/PMC8401387/ /pubmed/34451621 http://dx.doi.org/10.3390/plants10081576 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Kuang-Teng
Hong, Ming-Chang
Wu, Yu-Sheng
Wu, Tsung-Meng
Agrobacterium-Mediated Genetic Transformation of Taiwanese Isolates of Lemna aequinoctialis
title Agrobacterium-Mediated Genetic Transformation of Taiwanese Isolates of Lemna aequinoctialis
title_full Agrobacterium-Mediated Genetic Transformation of Taiwanese Isolates of Lemna aequinoctialis
title_fullStr Agrobacterium-Mediated Genetic Transformation of Taiwanese Isolates of Lemna aequinoctialis
title_full_unstemmed Agrobacterium-Mediated Genetic Transformation of Taiwanese Isolates of Lemna aequinoctialis
title_short Agrobacterium-Mediated Genetic Transformation of Taiwanese Isolates of Lemna aequinoctialis
title_sort agrobacterium-mediated genetic transformation of taiwanese isolates of lemna aequinoctialis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401387/
https://www.ncbi.nlm.nih.gov/pubmed/34451621
http://dx.doi.org/10.3390/plants10081576
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