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Beneficial Effect of Quercetin on Erythrocyte Properties in Type 2 Diabetic Rats

Diabetes mellitus is characterized by tissue oxidative damage and impaired microcirculation, as well as worsened erythrocyte properties. Measurements of erythrocyte deformability together with determination of nitric oxide (NO) production and osmotic resistance were used for the characterization of...

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Autores principales: Jasenovec, Tomas, Radosinska, Dominika, Kollarova, Marta, Balis, Peter, Ferenczyova, Kristina, Kalocayova, Barbora, Bartekova, Monika, Tothova, Lubomira, Radosinska, Jana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401571/
https://www.ncbi.nlm.nih.gov/pubmed/34443451
http://dx.doi.org/10.3390/molecules26164868
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author Jasenovec, Tomas
Radosinska, Dominika
Kollarova, Marta
Balis, Peter
Ferenczyova, Kristina
Kalocayova, Barbora
Bartekova, Monika
Tothova, Lubomira
Radosinska, Jana
author_facet Jasenovec, Tomas
Radosinska, Dominika
Kollarova, Marta
Balis, Peter
Ferenczyova, Kristina
Kalocayova, Barbora
Bartekova, Monika
Tothova, Lubomira
Radosinska, Jana
author_sort Jasenovec, Tomas
collection PubMed
description Diabetes mellitus is characterized by tissue oxidative damage and impaired microcirculation, as well as worsened erythrocyte properties. Measurements of erythrocyte deformability together with determination of nitric oxide (NO) production and osmotic resistance were used for the characterization of erythrocyte functionality in lean (control) and obese Zucker diabetic fatty (ZDF) rats of two age categories. Obese ZDF rats correspond to prediabetic (younger) and diabetic (older) animals. As antioxidants were suggested to protect erythrocytes, we also investigated the potential effect of quercetin (20 mg/kg/day for 6 weeks). Erythrocyte deformability was determined by the filtration method and NO production using DAF-2DA fluorescence. For erythrocyte osmotic resistance, we used hemolytic assay. Erythrocyte deformability and NO production deteriorated during aging—both were lower in older ZDF rats than in younger ones. Three-way ANOVA indicates improved erythrocyte deformability after quercetin treatment in older obese ZDF rats only, as it was not modified or deteriorated in both (lean and obese) younger and older lean animals. NO production by erythrocytes increased post treatment in all experimental groups. Our study indicates the potential benefit of quercetin treatment on erythrocyte properties in condition of diabetes mellitus. In addition, our results suggest potential age-dependency of quercetin effects in diabetes that deserve additional research.
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spelling pubmed-84015712021-08-29 Beneficial Effect of Quercetin on Erythrocyte Properties in Type 2 Diabetic Rats Jasenovec, Tomas Radosinska, Dominika Kollarova, Marta Balis, Peter Ferenczyova, Kristina Kalocayova, Barbora Bartekova, Monika Tothova, Lubomira Radosinska, Jana Molecules Article Diabetes mellitus is characterized by tissue oxidative damage and impaired microcirculation, as well as worsened erythrocyte properties. Measurements of erythrocyte deformability together with determination of nitric oxide (NO) production and osmotic resistance were used for the characterization of erythrocyte functionality in lean (control) and obese Zucker diabetic fatty (ZDF) rats of two age categories. Obese ZDF rats correspond to prediabetic (younger) and diabetic (older) animals. As antioxidants were suggested to protect erythrocytes, we also investigated the potential effect of quercetin (20 mg/kg/day for 6 weeks). Erythrocyte deformability was determined by the filtration method and NO production using DAF-2DA fluorescence. For erythrocyte osmotic resistance, we used hemolytic assay. Erythrocyte deformability and NO production deteriorated during aging—both were lower in older ZDF rats than in younger ones. Three-way ANOVA indicates improved erythrocyte deformability after quercetin treatment in older obese ZDF rats only, as it was not modified or deteriorated in both (lean and obese) younger and older lean animals. NO production by erythrocytes increased post treatment in all experimental groups. Our study indicates the potential benefit of quercetin treatment on erythrocyte properties in condition of diabetes mellitus. In addition, our results suggest potential age-dependency of quercetin effects in diabetes that deserve additional research. MDPI 2021-08-11 /pmc/articles/PMC8401571/ /pubmed/34443451 http://dx.doi.org/10.3390/molecules26164868 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jasenovec, Tomas
Radosinska, Dominika
Kollarova, Marta
Balis, Peter
Ferenczyova, Kristina
Kalocayova, Barbora
Bartekova, Monika
Tothova, Lubomira
Radosinska, Jana
Beneficial Effect of Quercetin on Erythrocyte Properties in Type 2 Diabetic Rats
title Beneficial Effect of Quercetin on Erythrocyte Properties in Type 2 Diabetic Rats
title_full Beneficial Effect of Quercetin on Erythrocyte Properties in Type 2 Diabetic Rats
title_fullStr Beneficial Effect of Quercetin on Erythrocyte Properties in Type 2 Diabetic Rats
title_full_unstemmed Beneficial Effect of Quercetin on Erythrocyte Properties in Type 2 Diabetic Rats
title_short Beneficial Effect of Quercetin on Erythrocyte Properties in Type 2 Diabetic Rats
title_sort beneficial effect of quercetin on erythrocyte properties in type 2 diabetic rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401571/
https://www.ncbi.nlm.nih.gov/pubmed/34443451
http://dx.doi.org/10.3390/molecules26164868
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