Cargando…

Development, Characterization and Cell Viability Inhibition of PVA Spheres Loaded with Doxorubicin and 4′-Amino-1-Naphthyl-Chalcone (D14) for Osteosarcoma

Chalcones (1,3-diaryl-2-propen-1-ones) are naturally occurring polyphenols with known anticancer activity against a variety of tumor cell lines, including osteosarcoma (OS). In this paper, we present the preparation and characterization of spheres (~2 mm) from polyvinyl alcohol (PVA) containing a co...

Descripción completa

Detalles Bibliográficos
Autores principales: Seba, Viviane, de Lima, Gabriel Goetten, Pereira, Bruno L., Silva, Gabriel, Reinhardt, Luiza Steffens, Arantes, Pablo Ricardo, Chee, Bor Shin, dos Santos, Mariana Bastos, França, Suzelei C., Regasini, Luis Octavio, Fachin, Ana Lúcia, Cao, Zhi, Nugent, Michael J. D., Marins, Mozart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401585/
https://www.ncbi.nlm.nih.gov/pubmed/34451151
http://dx.doi.org/10.3390/polym13162611
_version_ 1783745585654792192
author Seba, Viviane
de Lima, Gabriel Goetten
Pereira, Bruno L.
Silva, Gabriel
Reinhardt, Luiza Steffens
Arantes, Pablo Ricardo
Chee, Bor Shin
dos Santos, Mariana Bastos
França, Suzelei C.
Regasini, Luis Octavio
Fachin, Ana Lúcia
Cao, Zhi
Nugent, Michael J. D.
Marins, Mozart
author_facet Seba, Viviane
de Lima, Gabriel Goetten
Pereira, Bruno L.
Silva, Gabriel
Reinhardt, Luiza Steffens
Arantes, Pablo Ricardo
Chee, Bor Shin
dos Santos, Mariana Bastos
França, Suzelei C.
Regasini, Luis Octavio
Fachin, Ana Lúcia
Cao, Zhi
Nugent, Michael J. D.
Marins, Mozart
author_sort Seba, Viviane
collection PubMed
description Chalcones (1,3-diaryl-2-propen-1-ones) are naturally occurring polyphenols with known anticancer activity against a variety of tumor cell lines, including osteosarcoma (OS). In this paper, we present the preparation and characterization of spheres (~2 mm) from polyvinyl alcohol (PVA) containing a combination of 4′-Amino-1-Naphthyl-Chalcone (D14) and doxorubicin, to act as a new polymeric dual-drug anticancer delivery. D14 is a potent inhibitor of osteosarcoma progression and, when combined with doxorubicin, presents a synergetic effect; hence, physically crosslinked PVA spheres loaded with D14 and doxorubicin were prepared using liquid nitrogen and six freeze–thawing cycles. Physical-chemical characterization using a scanning electron microscope (SEM), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) presented that the drugs were incorporated into the spheres via weak interactions between the drugs and the polymeric chains, resulting in overall good drug stability. The cytotoxicity activity of the PVA spheres co-encapsulating both drugs was tested against the U2OS human osteosarcoma cell line by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay, and compared to the spheres carrying either D14 or doxorubicin alone. The co-delivery showed a cytotoxic effect 2.6-fold greater than doxorubicin alone, revealing a significant synergistic effect with a coefficient of drug interaction (CDI) of 0.49. The obtained results suggest this developed PVA sphere as a potential dual-drug delivery system that could be used for the prominent synergistic anticancer activity of co-delivering D14 and doxorubicin, providing a new potential strategy for improved osteosarcoma treatment.
format Online
Article
Text
id pubmed-8401585
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84015852021-08-29 Development, Characterization and Cell Viability Inhibition of PVA Spheres Loaded with Doxorubicin and 4′-Amino-1-Naphthyl-Chalcone (D14) for Osteosarcoma Seba, Viviane de Lima, Gabriel Goetten Pereira, Bruno L. Silva, Gabriel Reinhardt, Luiza Steffens Arantes, Pablo Ricardo Chee, Bor Shin dos Santos, Mariana Bastos França, Suzelei C. Regasini, Luis Octavio Fachin, Ana Lúcia Cao, Zhi Nugent, Michael J. D. Marins, Mozart Polymers (Basel) Article Chalcones (1,3-diaryl-2-propen-1-ones) are naturally occurring polyphenols with known anticancer activity against a variety of tumor cell lines, including osteosarcoma (OS). In this paper, we present the preparation and characterization of spheres (~2 mm) from polyvinyl alcohol (PVA) containing a combination of 4′-Amino-1-Naphthyl-Chalcone (D14) and doxorubicin, to act as a new polymeric dual-drug anticancer delivery. D14 is a potent inhibitor of osteosarcoma progression and, when combined with doxorubicin, presents a synergetic effect; hence, physically crosslinked PVA spheres loaded with D14 and doxorubicin were prepared using liquid nitrogen and six freeze–thawing cycles. Physical-chemical characterization using a scanning electron microscope (SEM), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) presented that the drugs were incorporated into the spheres via weak interactions between the drugs and the polymeric chains, resulting in overall good drug stability. The cytotoxicity activity of the PVA spheres co-encapsulating both drugs was tested against the U2OS human osteosarcoma cell line by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay, and compared to the spheres carrying either D14 or doxorubicin alone. The co-delivery showed a cytotoxic effect 2.6-fold greater than doxorubicin alone, revealing a significant synergistic effect with a coefficient of drug interaction (CDI) of 0.49. The obtained results suggest this developed PVA sphere as a potential dual-drug delivery system that could be used for the prominent synergistic anticancer activity of co-delivering D14 and doxorubicin, providing a new potential strategy for improved osteosarcoma treatment. MDPI 2021-08-06 /pmc/articles/PMC8401585/ /pubmed/34451151 http://dx.doi.org/10.3390/polym13162611 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Seba, Viviane
de Lima, Gabriel Goetten
Pereira, Bruno L.
Silva, Gabriel
Reinhardt, Luiza Steffens
Arantes, Pablo Ricardo
Chee, Bor Shin
dos Santos, Mariana Bastos
França, Suzelei C.
Regasini, Luis Octavio
Fachin, Ana Lúcia
Cao, Zhi
Nugent, Michael J. D.
Marins, Mozart
Development, Characterization and Cell Viability Inhibition of PVA Spheres Loaded with Doxorubicin and 4′-Amino-1-Naphthyl-Chalcone (D14) for Osteosarcoma
title Development, Characterization and Cell Viability Inhibition of PVA Spheres Loaded with Doxorubicin and 4′-Amino-1-Naphthyl-Chalcone (D14) for Osteosarcoma
title_full Development, Characterization and Cell Viability Inhibition of PVA Spheres Loaded with Doxorubicin and 4′-Amino-1-Naphthyl-Chalcone (D14) for Osteosarcoma
title_fullStr Development, Characterization and Cell Viability Inhibition of PVA Spheres Loaded with Doxorubicin and 4′-Amino-1-Naphthyl-Chalcone (D14) for Osteosarcoma
title_full_unstemmed Development, Characterization and Cell Viability Inhibition of PVA Spheres Loaded with Doxorubicin and 4′-Amino-1-Naphthyl-Chalcone (D14) for Osteosarcoma
title_short Development, Characterization and Cell Viability Inhibition of PVA Spheres Loaded with Doxorubicin and 4′-Amino-1-Naphthyl-Chalcone (D14) for Osteosarcoma
title_sort development, characterization and cell viability inhibition of pva spheres loaded with doxorubicin and 4′-amino-1-naphthyl-chalcone (d14) for osteosarcoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401585/
https://www.ncbi.nlm.nih.gov/pubmed/34451151
http://dx.doi.org/10.3390/polym13162611
work_keys_str_mv AT sebaviviane developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT delimagabrielgoetten developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT pereirabrunol developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT silvagabriel developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT reinhardtluizasteffens developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT arantespabloricardo developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT cheeborshin developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT dossantosmarianabastos developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT francasuzeleic developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT regasiniluisoctavio developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT fachinanalucia developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT caozhi developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT nugentmichaeljd developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma
AT marinsmozart developmentcharacterizationandcellviabilityinhibitionofpvaspheresloadedwithdoxorubicinand4amino1naphthylchalconed14forosteosarcoma