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The Physicochemical, Biopharmaceutical, and In Vitro Efficacy Properties of Freeze-Dried Dexamethasone-Loaded Lipomers

Dexamethasone-loaded polymer hybrid nanoparticles were developed as a potential tool to treat alopecia areata due to their follicular targeting ability. Freeze drying (FD) is a common technique used to improve nanoparticle stability; however, there are few studies focused on its effect on ethyl cell...

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Autores principales: Pena-Rodríguez, Eloy, Mata-Ventosa, Aida, Garcia-Vega, Laura, Pérez-Torras, Sandra, Fernández-Campos, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401638/
https://www.ncbi.nlm.nih.gov/pubmed/34452283
http://dx.doi.org/10.3390/pharmaceutics13081322
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author Pena-Rodríguez, Eloy
Mata-Ventosa, Aida
Garcia-Vega, Laura
Pérez-Torras, Sandra
Fernández-Campos, Francisco
author_facet Pena-Rodríguez, Eloy
Mata-Ventosa, Aida
Garcia-Vega, Laura
Pérez-Torras, Sandra
Fernández-Campos, Francisco
author_sort Pena-Rodríguez, Eloy
collection PubMed
description Dexamethasone-loaded polymer hybrid nanoparticles were developed as a potential tool to treat alopecia areata due to their follicular targeting ability. Freeze drying (FD) is a common technique used to improve nanoparticle stability; however, there are few studies focused on its effect on ethyl cellulose lipid-core nanoparticles. Nanoparticles were lyophilized with different cryoprotectants. Sucrose was selected because it allowed for a good resuspension and provided acceptable physicochemical parameters (374.33 nm, +34.7 mV, polydispersion 0.229%, and 98.87% encapsulation efficiency). The nanoparticles obtained were loaded into a pleasant xanthan gum hydrogel, and the rheological, release, and skin permeation profiles of different formulations were studied. The FD formulation significantly modified the particle size, and the drug release and permeation properties were also altered. In addition, analyses of the cytotoxicity and anti-inflammatory efficacy of FD and non-FD particles on human keratinocytes indicated no differences.
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spelling pubmed-84016382021-08-29 The Physicochemical, Biopharmaceutical, and In Vitro Efficacy Properties of Freeze-Dried Dexamethasone-Loaded Lipomers Pena-Rodríguez, Eloy Mata-Ventosa, Aida Garcia-Vega, Laura Pérez-Torras, Sandra Fernández-Campos, Francisco Pharmaceutics Article Dexamethasone-loaded polymer hybrid nanoparticles were developed as a potential tool to treat alopecia areata due to their follicular targeting ability. Freeze drying (FD) is a common technique used to improve nanoparticle stability; however, there are few studies focused on its effect on ethyl cellulose lipid-core nanoparticles. Nanoparticles were lyophilized with different cryoprotectants. Sucrose was selected because it allowed for a good resuspension and provided acceptable physicochemical parameters (374.33 nm, +34.7 mV, polydispersion 0.229%, and 98.87% encapsulation efficiency). The nanoparticles obtained were loaded into a pleasant xanthan gum hydrogel, and the rheological, release, and skin permeation profiles of different formulations were studied. The FD formulation significantly modified the particle size, and the drug release and permeation properties were also altered. In addition, analyses of the cytotoxicity and anti-inflammatory efficacy of FD and non-FD particles on human keratinocytes indicated no differences. MDPI 2021-08-23 /pmc/articles/PMC8401638/ /pubmed/34452283 http://dx.doi.org/10.3390/pharmaceutics13081322 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pena-Rodríguez, Eloy
Mata-Ventosa, Aida
Garcia-Vega, Laura
Pérez-Torras, Sandra
Fernández-Campos, Francisco
The Physicochemical, Biopharmaceutical, and In Vitro Efficacy Properties of Freeze-Dried Dexamethasone-Loaded Lipomers
title The Physicochemical, Biopharmaceutical, and In Vitro Efficacy Properties of Freeze-Dried Dexamethasone-Loaded Lipomers
title_full The Physicochemical, Biopharmaceutical, and In Vitro Efficacy Properties of Freeze-Dried Dexamethasone-Loaded Lipomers
title_fullStr The Physicochemical, Biopharmaceutical, and In Vitro Efficacy Properties of Freeze-Dried Dexamethasone-Loaded Lipomers
title_full_unstemmed The Physicochemical, Biopharmaceutical, and In Vitro Efficacy Properties of Freeze-Dried Dexamethasone-Loaded Lipomers
title_short The Physicochemical, Biopharmaceutical, and In Vitro Efficacy Properties of Freeze-Dried Dexamethasone-Loaded Lipomers
title_sort physicochemical, biopharmaceutical, and in vitro efficacy properties of freeze-dried dexamethasone-loaded lipomers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401638/
https://www.ncbi.nlm.nih.gov/pubmed/34452283
http://dx.doi.org/10.3390/pharmaceutics13081322
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