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Genomic Insights into Drug Resistance Determinants in Cedecea neteri, A Rare Opportunistic Pathogen
Cedecea, a genus in the Enterobacteriaceae family, includes several opportunistic pathogens reported to cause an array of sporadic acute infections, most notably of the lung and bloodstream. One species, Cedecea neteri, is associated with cases of bacteremia in immunocompromised hosts and has docume...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401664/ https://www.ncbi.nlm.nih.gov/pubmed/34442820 http://dx.doi.org/10.3390/microorganisms9081741 |
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author | Thompson, Dorothea K. Sharkady, Stephen M. |
author_facet | Thompson, Dorothea K. Sharkady, Stephen M. |
author_sort | Thompson, Dorothea K. |
collection | PubMed |
description | Cedecea, a genus in the Enterobacteriaceae family, includes several opportunistic pathogens reported to cause an array of sporadic acute infections, most notably of the lung and bloodstream. One species, Cedecea neteri, is associated with cases of bacteremia in immunocompromised hosts and has documented resistance to different antibiotics, including β-lactams and colistin. Despite the potential to inflict serious infections, knowledge about drug resistance determinants in Cedecea is limited. In this study, we utilized whole-genome sequence data available for three environmental strains (SSMD04, M006, ND14a) of C. neteri and various bioinformatics tools to analyze drug resistance genes in this bacterium. All three genomes harbor multiple chromosome-encoded β-lactamase genes. A deeper analysis of β-lactamase genes in SSMD04 revealed four metallo-β-lactamases, a novel variant, and a CMY/ACT-type AmpC putatively regulated by a divergently transcribed AmpR. Homologs of known resistance-nodulation-cell division (RND)-type multidrug efflux pumps such as OqxB, AcrB, AcrD, and MdtBC were also identified. Genomic island prediction for SSMD04 indicated that tolC, involved in drug and toxin export across the outer membrane of Gram-negative bacteria, was acquired by a transposase-mediated genetic transfer mechanism. Our study provides new insights into drug resistance mechanisms of an environmental microorganism capable of behaving as a clinically relevant opportunistic pathogen. |
format | Online Article Text |
id | pubmed-8401664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84016642021-08-29 Genomic Insights into Drug Resistance Determinants in Cedecea neteri, A Rare Opportunistic Pathogen Thompson, Dorothea K. Sharkady, Stephen M. Microorganisms Article Cedecea, a genus in the Enterobacteriaceae family, includes several opportunistic pathogens reported to cause an array of sporadic acute infections, most notably of the lung and bloodstream. One species, Cedecea neteri, is associated with cases of bacteremia in immunocompromised hosts and has documented resistance to different antibiotics, including β-lactams and colistin. Despite the potential to inflict serious infections, knowledge about drug resistance determinants in Cedecea is limited. In this study, we utilized whole-genome sequence data available for three environmental strains (SSMD04, M006, ND14a) of C. neteri and various bioinformatics tools to analyze drug resistance genes in this bacterium. All three genomes harbor multiple chromosome-encoded β-lactamase genes. A deeper analysis of β-lactamase genes in SSMD04 revealed four metallo-β-lactamases, a novel variant, and a CMY/ACT-type AmpC putatively regulated by a divergently transcribed AmpR. Homologs of known resistance-nodulation-cell division (RND)-type multidrug efflux pumps such as OqxB, AcrB, AcrD, and MdtBC were also identified. Genomic island prediction for SSMD04 indicated that tolC, involved in drug and toxin export across the outer membrane of Gram-negative bacteria, was acquired by a transposase-mediated genetic transfer mechanism. Our study provides new insights into drug resistance mechanisms of an environmental microorganism capable of behaving as a clinically relevant opportunistic pathogen. MDPI 2021-08-15 /pmc/articles/PMC8401664/ /pubmed/34442820 http://dx.doi.org/10.3390/microorganisms9081741 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Thompson, Dorothea K. Sharkady, Stephen M. Genomic Insights into Drug Resistance Determinants in Cedecea neteri, A Rare Opportunistic Pathogen |
title | Genomic Insights into Drug Resistance Determinants in Cedecea neteri, A Rare Opportunistic Pathogen |
title_full | Genomic Insights into Drug Resistance Determinants in Cedecea neteri, A Rare Opportunistic Pathogen |
title_fullStr | Genomic Insights into Drug Resistance Determinants in Cedecea neteri, A Rare Opportunistic Pathogen |
title_full_unstemmed | Genomic Insights into Drug Resistance Determinants in Cedecea neteri, A Rare Opportunistic Pathogen |
title_short | Genomic Insights into Drug Resistance Determinants in Cedecea neteri, A Rare Opportunistic Pathogen |
title_sort | genomic insights into drug resistance determinants in cedecea neteri, a rare opportunistic pathogen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401664/ https://www.ncbi.nlm.nih.gov/pubmed/34442820 http://dx.doi.org/10.3390/microorganisms9081741 |
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