Presence of SARS-CoV-2 in a Cornea Transplant

Background: The SARS-CoV-2 pandemic has impacted tissue transplantation procedures since conjunctivas were found to be associated with coronavirus infection. Here, we investigated infection of a cornea graft from a COVID-19-positive donor. Methods: In order to evaluate the presence of SARS-CoV-2 in...

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Detalles Bibliográficos
Autores principales: Otmani Idrissi, Myriem, Baudoin, Jean-Pierre, Chateau, Anne-Line, Aherfi, Sarah, Bedotto-Buffet, Marielle, Latil, Alain, Lepidi, Hubert, Chiaroni, Jacques, Picard, Christophe, Mege, Jean-Louis, La Scola, Bernard, Mezouar, Soraya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401771/
https://www.ncbi.nlm.nih.gov/pubmed/34451398
http://dx.doi.org/10.3390/pathogens10080934
Descripción
Sumario:Background: The SARS-CoV-2 pandemic has impacted tissue transplantation procedures since conjunctivas were found to be associated with coronavirus infection. Here, we investigated infection of a cornea graft from a COVID-19-positive donor. Methods: In order to evaluate the presence of SARS-CoV-2 in the cornea graft we first carried out a qRT-PCR and then we investigated the presence of SARS-CoV-2 by fluorescence and electron microscopy. Conclusions: Although the cornea graft was found to be negative by qRT-PCR, we were able to show the presence of SARS-CoV-2 in corneal cells expressing the SARS-CoV-2 receptor, ACE2. Taken together, our findings may have important implications for the use of corneal tissue in graft indications and open the debate on SARS-CoV-2 transmissibility.

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