Presence of SARS-CoV-2 in a Cornea Transplant

Background: The SARS-CoV-2 pandemic has impacted tissue transplantation procedures since conjunctivas were found to be associated with coronavirus infection. Here, we investigated infection of a cornea graft from a COVID-19-positive donor. Methods: In order to evaluate the presence of SARS-CoV-2 in...

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Autores principales: Otmani Idrissi, Myriem, Baudoin, Jean-Pierre, Chateau, Anne-Line, Aherfi, Sarah, Bedotto-Buffet, Marielle, Latil, Alain, Lepidi, Hubert, Chiaroni, Jacques, Picard, Christophe, Mege, Jean-Louis, La Scola, Bernard, Mezouar, Soraya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401771/
https://www.ncbi.nlm.nih.gov/pubmed/34451398
http://dx.doi.org/10.3390/pathogens10080934
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author Otmani Idrissi, Myriem
Baudoin, Jean-Pierre
Chateau, Anne-Line
Aherfi, Sarah
Bedotto-Buffet, Marielle
Latil, Alain
Lepidi, Hubert
Chiaroni, Jacques
Picard, Christophe
Mege, Jean-Louis
La Scola, Bernard
Mezouar, Soraya
author_facet Otmani Idrissi, Myriem
Baudoin, Jean-Pierre
Chateau, Anne-Line
Aherfi, Sarah
Bedotto-Buffet, Marielle
Latil, Alain
Lepidi, Hubert
Chiaroni, Jacques
Picard, Christophe
Mege, Jean-Louis
La Scola, Bernard
Mezouar, Soraya
author_sort Otmani Idrissi, Myriem
collection PubMed
description Background: The SARS-CoV-2 pandemic has impacted tissue transplantation procedures since conjunctivas were found to be associated with coronavirus infection. Here, we investigated infection of a cornea graft from a COVID-19-positive donor. Methods: In order to evaluate the presence of SARS-CoV-2 in the cornea graft we first carried out a qRT-PCR and then we investigated the presence of SARS-CoV-2 by fluorescence and electron microscopy. Conclusions: Although the cornea graft was found to be negative by qRT-PCR, we were able to show the presence of SARS-CoV-2 in corneal cells expressing the SARS-CoV-2 receptor, ACE2. Taken together, our findings may have important implications for the use of corneal tissue in graft indications and open the debate on SARS-CoV-2 transmissibility.
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spelling pubmed-84017712021-08-29 Presence of SARS-CoV-2 in a Cornea Transplant Otmani Idrissi, Myriem Baudoin, Jean-Pierre Chateau, Anne-Line Aherfi, Sarah Bedotto-Buffet, Marielle Latil, Alain Lepidi, Hubert Chiaroni, Jacques Picard, Christophe Mege, Jean-Louis La Scola, Bernard Mezouar, Soraya Pathogens Brief Report Background: The SARS-CoV-2 pandemic has impacted tissue transplantation procedures since conjunctivas were found to be associated with coronavirus infection. Here, we investigated infection of a cornea graft from a COVID-19-positive donor. Methods: In order to evaluate the presence of SARS-CoV-2 in the cornea graft we first carried out a qRT-PCR and then we investigated the presence of SARS-CoV-2 by fluorescence and electron microscopy. Conclusions: Although the cornea graft was found to be negative by qRT-PCR, we were able to show the presence of SARS-CoV-2 in corneal cells expressing the SARS-CoV-2 receptor, ACE2. Taken together, our findings may have important implications for the use of corneal tissue in graft indications and open the debate on SARS-CoV-2 transmissibility. MDPI 2021-07-24 /pmc/articles/PMC8401771/ /pubmed/34451398 http://dx.doi.org/10.3390/pathogens10080934 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Otmani Idrissi, Myriem
Baudoin, Jean-Pierre
Chateau, Anne-Line
Aherfi, Sarah
Bedotto-Buffet, Marielle
Latil, Alain
Lepidi, Hubert
Chiaroni, Jacques
Picard, Christophe
Mege, Jean-Louis
La Scola, Bernard
Mezouar, Soraya
Presence of SARS-CoV-2 in a Cornea Transplant
title Presence of SARS-CoV-2 in a Cornea Transplant
title_full Presence of SARS-CoV-2 in a Cornea Transplant
title_fullStr Presence of SARS-CoV-2 in a Cornea Transplant
title_full_unstemmed Presence of SARS-CoV-2 in a Cornea Transplant
title_short Presence of SARS-CoV-2 in a Cornea Transplant
title_sort presence of sars-cov-2 in a cornea transplant
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401771/
https://www.ncbi.nlm.nih.gov/pubmed/34451398
http://dx.doi.org/10.3390/pathogens10080934
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