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Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood
Circulating tumor cells (CTCs) isolation from a blood sample plays an important role in cancer diagnosis and treatment. Microfluidics offers a great potential for cancer cell separation from the blood. Among the microfluidic-based methods for CTC separation, the inertial method as a passive method a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401779/ https://www.ncbi.nlm.nih.gov/pubmed/34442499 http://dx.doi.org/10.3390/mi12080877 |
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author | Nasiri, Rohollah Shamloo, Amir Akbari, Javad |
author_facet | Nasiri, Rohollah Shamloo, Amir Akbari, Javad |
author_sort | Nasiri, Rohollah |
collection | PubMed |
description | Circulating tumor cells (CTCs) isolation from a blood sample plays an important role in cancer diagnosis and treatment. Microfluidics offers a great potential for cancer cell separation from the blood. Among the microfluidic-based methods for CTC separation, the inertial method as a passive method and magnetic method as an active method are two efficient well-established methods. Here, we investigated the combination of these two methods to separate CTCs from a blood sample in a single chip. Firstly, numerical simulations were performed to analyze the fluid flow within the proposed channel, and the particle trajectories within the inertial cell separation unit were investigated to determine/predict the particle trajectories within the inertial channel in the presence of fluid dynamic forces. Then, the designed device was fabricated using the soft-lithography technique. Later, the CTCs were conjugated with magnetic nanoparticles and Ep-CAM antibodies to improve the magnetic susceptibility of the cells in the presence of a magnetic field by using neodymium permanent magnets of 0.51 T. A diluted blood sample containing nanoparticle-conjugated CTCs was injected into the device at different flow rates to analyze its performance. It was found that the flow rate of 1000 µL/min resulted in the highest recovery rate and purity of ~95% and ~93% for CTCs, respectively. |
format | Online Article Text |
id | pubmed-8401779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84017792021-08-29 Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood Nasiri, Rohollah Shamloo, Amir Akbari, Javad Micromachines (Basel) Article Circulating tumor cells (CTCs) isolation from a blood sample plays an important role in cancer diagnosis and treatment. Microfluidics offers a great potential for cancer cell separation from the blood. Among the microfluidic-based methods for CTC separation, the inertial method as a passive method and magnetic method as an active method are two efficient well-established methods. Here, we investigated the combination of these two methods to separate CTCs from a blood sample in a single chip. Firstly, numerical simulations were performed to analyze the fluid flow within the proposed channel, and the particle trajectories within the inertial cell separation unit were investigated to determine/predict the particle trajectories within the inertial channel in the presence of fluid dynamic forces. Then, the designed device was fabricated using the soft-lithography technique. Later, the CTCs were conjugated with magnetic nanoparticles and Ep-CAM antibodies to improve the magnetic susceptibility of the cells in the presence of a magnetic field by using neodymium permanent magnets of 0.51 T. A diluted blood sample containing nanoparticle-conjugated CTCs was injected into the device at different flow rates to analyze its performance. It was found that the flow rate of 1000 µL/min resulted in the highest recovery rate and purity of ~95% and ~93% for CTCs, respectively. MDPI 2021-07-26 /pmc/articles/PMC8401779/ /pubmed/34442499 http://dx.doi.org/10.3390/mi12080877 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nasiri, Rohollah Shamloo, Amir Akbari, Javad Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood |
title | Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood |
title_full | Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood |
title_fullStr | Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood |
title_full_unstemmed | Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood |
title_short | Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood |
title_sort | design of a hybrid inertial and magnetophoretic microfluidic device for ctcs separation from blood |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401779/ https://www.ncbi.nlm.nih.gov/pubmed/34442499 http://dx.doi.org/10.3390/mi12080877 |
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