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Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood

Circulating tumor cells (CTCs) isolation from a blood sample plays an important role in cancer diagnosis and treatment. Microfluidics offers a great potential for cancer cell separation from the blood. Among the microfluidic-based methods for CTC separation, the inertial method as a passive method a...

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Autores principales: Nasiri, Rohollah, Shamloo, Amir, Akbari, Javad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401779/
https://www.ncbi.nlm.nih.gov/pubmed/34442499
http://dx.doi.org/10.3390/mi12080877
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author Nasiri, Rohollah
Shamloo, Amir
Akbari, Javad
author_facet Nasiri, Rohollah
Shamloo, Amir
Akbari, Javad
author_sort Nasiri, Rohollah
collection PubMed
description Circulating tumor cells (CTCs) isolation from a blood sample plays an important role in cancer diagnosis and treatment. Microfluidics offers a great potential for cancer cell separation from the blood. Among the microfluidic-based methods for CTC separation, the inertial method as a passive method and magnetic method as an active method are two efficient well-established methods. Here, we investigated the combination of these two methods to separate CTCs from a blood sample in a single chip. Firstly, numerical simulations were performed to analyze the fluid flow within the proposed channel, and the particle trajectories within the inertial cell separation unit were investigated to determine/predict the particle trajectories within the inertial channel in the presence of fluid dynamic forces. Then, the designed device was fabricated using the soft-lithography technique. Later, the CTCs were conjugated with magnetic nanoparticles and Ep-CAM antibodies to improve the magnetic susceptibility of the cells in the presence of a magnetic field by using neodymium permanent magnets of 0.51 T. A diluted blood sample containing nanoparticle-conjugated CTCs was injected into the device at different flow rates to analyze its performance. It was found that the flow rate of 1000 µL/min resulted in the highest recovery rate and purity of ~95% and ~93% for CTCs, respectively.
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spelling pubmed-84017792021-08-29 Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood Nasiri, Rohollah Shamloo, Amir Akbari, Javad Micromachines (Basel) Article Circulating tumor cells (CTCs) isolation from a blood sample plays an important role in cancer diagnosis and treatment. Microfluidics offers a great potential for cancer cell separation from the blood. Among the microfluidic-based methods for CTC separation, the inertial method as a passive method and magnetic method as an active method are two efficient well-established methods. Here, we investigated the combination of these two methods to separate CTCs from a blood sample in a single chip. Firstly, numerical simulations were performed to analyze the fluid flow within the proposed channel, and the particle trajectories within the inertial cell separation unit were investigated to determine/predict the particle trajectories within the inertial channel in the presence of fluid dynamic forces. Then, the designed device was fabricated using the soft-lithography technique. Later, the CTCs were conjugated with magnetic nanoparticles and Ep-CAM antibodies to improve the magnetic susceptibility of the cells in the presence of a magnetic field by using neodymium permanent magnets of 0.51 T. A diluted blood sample containing nanoparticle-conjugated CTCs was injected into the device at different flow rates to analyze its performance. It was found that the flow rate of 1000 µL/min resulted in the highest recovery rate and purity of ~95% and ~93% for CTCs, respectively. MDPI 2021-07-26 /pmc/articles/PMC8401779/ /pubmed/34442499 http://dx.doi.org/10.3390/mi12080877 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nasiri, Rohollah
Shamloo, Amir
Akbari, Javad
Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood
title Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood
title_full Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood
title_fullStr Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood
title_full_unstemmed Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood
title_short Design of a Hybrid Inertial and Magnetophoretic Microfluidic Device for CTCs Separation from Blood
title_sort design of a hybrid inertial and magnetophoretic microfluidic device for ctcs separation from blood
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401779/
https://www.ncbi.nlm.nih.gov/pubmed/34442499
http://dx.doi.org/10.3390/mi12080877
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