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Reduction of In Vivo Placental Amino Acid Transport Precedes the Development of Intrauterine Growth Restriction in the Non-Human Primate

Intrauterine growth restriction (IUGR) is associated with reduced placental amino acid transport (AAT). However, it remains to be established if changes in AAT contribute to restricted fetal growth. We hypothesized that reduced in vivo placental AAT precedes the development of IUGR in baboons with m...

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Autores principales: Rosario, Fredrick J., Kramer, Anita, Li, Cun, Galan, Henry L., Powell, Theresa L., Nathanielsz, Peter W., Jansson, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401823/
https://www.ncbi.nlm.nih.gov/pubmed/34445051
http://dx.doi.org/10.3390/nu13082892
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author Rosario, Fredrick J.
Kramer, Anita
Li, Cun
Galan, Henry L.
Powell, Theresa L.
Nathanielsz, Peter W.
Jansson, Thomas
author_facet Rosario, Fredrick J.
Kramer, Anita
Li, Cun
Galan, Henry L.
Powell, Theresa L.
Nathanielsz, Peter W.
Jansson, Thomas
author_sort Rosario, Fredrick J.
collection PubMed
description Intrauterine growth restriction (IUGR) is associated with reduced placental amino acid transport (AAT). However, it remains to be established if changes in AAT contribute to restricted fetal growth. We hypothesized that reduced in vivo placental AAT precedes the development of IUGR in baboons with maternal nutrient restriction (MNR). Baboons were fed either a control (ad libitum) or MNR diet (70% of control diet) from gestational day (GD) 30. At GD 140, in vivo transplacental AA transport was measured by infusing nine ((13))C- or ((2))H-labeled essential amino acids (EAAs) as a bolus into the maternal circulation at cesarean section. A fetal vein-to-maternal artery mole percent excess ratio for each EAA was measured. Microvillous plasma membrane (MVM) system A and system L transport activity were determined. Fetal and placental weights were not significantly different between MNR and control. In vivo, the fetal vein-to-maternal artery mole percent excess ratio was significantly decreased for tryptophan in MNR. MVM system A and system L activity was markedly reduced in MNR. Reduction of in vivo placental amino acid transport precedes fetal growth restriction in the non-human primate, suggesting that reduced placental amino acid transfer may contribute to IUGR.
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spelling pubmed-84018232021-08-29 Reduction of In Vivo Placental Amino Acid Transport Precedes the Development of Intrauterine Growth Restriction in the Non-Human Primate Rosario, Fredrick J. Kramer, Anita Li, Cun Galan, Henry L. Powell, Theresa L. Nathanielsz, Peter W. Jansson, Thomas Nutrients Article Intrauterine growth restriction (IUGR) is associated with reduced placental amino acid transport (AAT). However, it remains to be established if changes in AAT contribute to restricted fetal growth. We hypothesized that reduced in vivo placental AAT precedes the development of IUGR in baboons with maternal nutrient restriction (MNR). Baboons were fed either a control (ad libitum) or MNR diet (70% of control diet) from gestational day (GD) 30. At GD 140, in vivo transplacental AA transport was measured by infusing nine ((13))C- or ((2))H-labeled essential amino acids (EAAs) as a bolus into the maternal circulation at cesarean section. A fetal vein-to-maternal artery mole percent excess ratio for each EAA was measured. Microvillous plasma membrane (MVM) system A and system L transport activity were determined. Fetal and placental weights were not significantly different between MNR and control. In vivo, the fetal vein-to-maternal artery mole percent excess ratio was significantly decreased for tryptophan in MNR. MVM system A and system L activity was markedly reduced in MNR. Reduction of in vivo placental amino acid transport precedes fetal growth restriction in the non-human primate, suggesting that reduced placental amino acid transfer may contribute to IUGR. MDPI 2021-08-23 /pmc/articles/PMC8401823/ /pubmed/34445051 http://dx.doi.org/10.3390/nu13082892 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rosario, Fredrick J.
Kramer, Anita
Li, Cun
Galan, Henry L.
Powell, Theresa L.
Nathanielsz, Peter W.
Jansson, Thomas
Reduction of In Vivo Placental Amino Acid Transport Precedes the Development of Intrauterine Growth Restriction in the Non-Human Primate
title Reduction of In Vivo Placental Amino Acid Transport Precedes the Development of Intrauterine Growth Restriction in the Non-Human Primate
title_full Reduction of In Vivo Placental Amino Acid Transport Precedes the Development of Intrauterine Growth Restriction in the Non-Human Primate
title_fullStr Reduction of In Vivo Placental Amino Acid Transport Precedes the Development of Intrauterine Growth Restriction in the Non-Human Primate
title_full_unstemmed Reduction of In Vivo Placental Amino Acid Transport Precedes the Development of Intrauterine Growth Restriction in the Non-Human Primate
title_short Reduction of In Vivo Placental Amino Acid Transport Precedes the Development of Intrauterine Growth Restriction in the Non-Human Primate
title_sort reduction of in vivo placental amino acid transport precedes the development of intrauterine growth restriction in the non-human primate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401823/
https://www.ncbi.nlm.nih.gov/pubmed/34445051
http://dx.doi.org/10.3390/nu13082892
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