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Cunninghamella arunalokei a New Species of Cunninghamella from India Causing Disease in an Immunocompetent Individual

Mucormycosis due to Cunninghamella spp. is a rare disease, especially in immunocompetent individuals. Here, we describe the isolation and characterization of a new species of Cunninghamella, causing chronic rhino-orbital-cerebral disease, and review cases of mucormycosis due to Cunninghamella spp. i...

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Autores principales: Hallur, Vinaykumar, Prakash, Hariprasath, Sable, Mukund, Preetam, Chappity, Purushotham, Prashanth, Senapati, Rabindra, Shankarnarayan, Shamanth Adekhandi, Bag, Nerbadyswari Deep, Rudramurthy, Shivaprakash Mandya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401845/
https://www.ncbi.nlm.nih.gov/pubmed/34436209
http://dx.doi.org/10.3390/jof7080670
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author Hallur, Vinaykumar
Prakash, Hariprasath
Sable, Mukund
Preetam, Chappity
Purushotham, Prashanth
Senapati, Rabindra
Shankarnarayan, Shamanth Adekhandi
Bag, Nerbadyswari Deep
Rudramurthy, Shivaprakash Mandya
author_facet Hallur, Vinaykumar
Prakash, Hariprasath
Sable, Mukund
Preetam, Chappity
Purushotham, Prashanth
Senapati, Rabindra
Shankarnarayan, Shamanth Adekhandi
Bag, Nerbadyswari Deep
Rudramurthy, Shivaprakash Mandya
author_sort Hallur, Vinaykumar
collection PubMed
description Mucormycosis due to Cunninghamella spp. is a rare disease, especially in immunocompetent individuals. Here, we describe the isolation and characterization of a new species of Cunninghamella, causing chronic rhino-orbital-cerebral disease, and review cases of mucormycosis due to Cunninghamella spp. in immunocompetent individuals. The Basic Local Alignment Search Tool (BLAST) analysis of the internal transcribed spacer region (ITS) sequence of isolate NCCPF 890012 showed 90% similarity with Cunninghamella bigelovii, while the large ribosomal subunit (28S) and translation elongation factor-1 alpha (EF-1 alpha) gene sequences showed 98% identity. Further, the phylogenetic analysis with concatenated sequences clustered isolate (NCCPF 890012) closely with C. bigelovii. The ITS sequence showed the maximum variation among three genes analyzed and helped in the new species’ delineation. Comparison of the assembled whole genome of NCCPF 890012 with other Mucorales using 123 single-copy orthologous genes showed clustering within the genus Cunninghamella. Based on these findings, the isolate is considered to be a new species of Cunninghamella and designated as Cunninghamella arunalokei sp. nov. Despite repeated debridement and antifungal treatment, the patient had multiple recurrences with intracranial extension and succumbed to the illness.
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spelling pubmed-84018452021-08-29 Cunninghamella arunalokei a New Species of Cunninghamella from India Causing Disease in an Immunocompetent Individual Hallur, Vinaykumar Prakash, Hariprasath Sable, Mukund Preetam, Chappity Purushotham, Prashanth Senapati, Rabindra Shankarnarayan, Shamanth Adekhandi Bag, Nerbadyswari Deep Rudramurthy, Shivaprakash Mandya J Fungi (Basel) Article Mucormycosis due to Cunninghamella spp. is a rare disease, especially in immunocompetent individuals. Here, we describe the isolation and characterization of a new species of Cunninghamella, causing chronic rhino-orbital-cerebral disease, and review cases of mucormycosis due to Cunninghamella spp. in immunocompetent individuals. The Basic Local Alignment Search Tool (BLAST) analysis of the internal transcribed spacer region (ITS) sequence of isolate NCCPF 890012 showed 90% similarity with Cunninghamella bigelovii, while the large ribosomal subunit (28S) and translation elongation factor-1 alpha (EF-1 alpha) gene sequences showed 98% identity. Further, the phylogenetic analysis with concatenated sequences clustered isolate (NCCPF 890012) closely with C. bigelovii. The ITS sequence showed the maximum variation among three genes analyzed and helped in the new species’ delineation. Comparison of the assembled whole genome of NCCPF 890012 with other Mucorales using 123 single-copy orthologous genes showed clustering within the genus Cunninghamella. Based on these findings, the isolate is considered to be a new species of Cunninghamella and designated as Cunninghamella arunalokei sp. nov. Despite repeated debridement and antifungal treatment, the patient had multiple recurrences with intracranial extension and succumbed to the illness. MDPI 2021-08-19 /pmc/articles/PMC8401845/ /pubmed/34436209 http://dx.doi.org/10.3390/jof7080670 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hallur, Vinaykumar
Prakash, Hariprasath
Sable, Mukund
Preetam, Chappity
Purushotham, Prashanth
Senapati, Rabindra
Shankarnarayan, Shamanth Adekhandi
Bag, Nerbadyswari Deep
Rudramurthy, Shivaprakash Mandya
Cunninghamella arunalokei a New Species of Cunninghamella from India Causing Disease in an Immunocompetent Individual
title Cunninghamella arunalokei a New Species of Cunninghamella from India Causing Disease in an Immunocompetent Individual
title_full Cunninghamella arunalokei a New Species of Cunninghamella from India Causing Disease in an Immunocompetent Individual
title_fullStr Cunninghamella arunalokei a New Species of Cunninghamella from India Causing Disease in an Immunocompetent Individual
title_full_unstemmed Cunninghamella arunalokei a New Species of Cunninghamella from India Causing Disease in an Immunocompetent Individual
title_short Cunninghamella arunalokei a New Species of Cunninghamella from India Causing Disease in an Immunocompetent Individual
title_sort cunninghamella arunalokei a new species of cunninghamella from india causing disease in an immunocompetent individual
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401845/
https://www.ncbi.nlm.nih.gov/pubmed/34436209
http://dx.doi.org/10.3390/jof7080670
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