Cargando…
A Novel Bioanalytical Method for Determination of Inotodiol Isolated from Inonotus Obliquus and Its Application to Pharmacokinetic Study
In this study, we developed a bioanalytical method using liquid chromatography coupled to triple quadrupole tandem mass spectrometry (LC-MS/MS) to apply to a pharmacokinetic study of inotodiol, which is known for its anti-cancer activity. Plasma samples were prepared with alkaline hydrolysis, liquid...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401913/ https://www.ncbi.nlm.nih.gov/pubmed/34451676 http://dx.doi.org/10.3390/plants10081631 |
_version_ | 1783745663365808128 |
---|---|
author | Kim, Jin Hyeok Gao, Dan Cho, Chong Woon Hwang, Inkyu Kim, Hyung Min Kang, Jong Seong |
author_facet | Kim, Jin Hyeok Gao, Dan Cho, Chong Woon Hwang, Inkyu Kim, Hyung Min Kang, Jong Seong |
author_sort | Kim, Jin Hyeok |
collection | PubMed |
description | In this study, we developed a bioanalytical method using liquid chromatography coupled to triple quadrupole tandem mass spectrometry (LC-MS/MS) to apply to a pharmacokinetic study of inotodiol, which is known for its anti-cancer activity. Plasma samples were prepared with alkaline hydrolysis, liquid–liquid extraction, and solid-phase extraction. Inotodiol was detected in positive mode with atmospheric pressure chemical ionization by multiple-reaction monitoring mode using LC-MS/MS. The developed method was validated with linearity, accuracy, and precision. Accuracy ranged from 97.8% to 111.9%, and the coefficient of variation for precision was 1.8% to 4.4%. The developed method was applied for pharmacokinetic study, and the mean pharmacokinetic parameters administration were calculated as follows: λ(z) 0.016 min(−1); T(1/2) 49.35 min; C(max) 2582 ng/mL; Cl 0.004 ng/min; AUC(0–t) 109,500 ng×min/mL; MRT(0–t) 32.30 min; Vd 0.281 mL after intravenous administration at dose of 2 mg/kg and λ(z) 0.005 min(−1); T(1/2) 138.6 min; T(max) 40 min; C(max) 49.56 ng/mL; AUC(0–t) 6176 ng×in/mL; MRT(0–t) 103.7 min after oral administration. The absolute oral bioavailability of inotodiol was 0.45%, similar to nonpolar phytosterols. Collectively, this is the first bioanalytical method and pharmacokinetic study for inotodiol. |
format | Online Article Text |
id | pubmed-8401913 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84019132021-08-29 A Novel Bioanalytical Method for Determination of Inotodiol Isolated from Inonotus Obliquus and Its Application to Pharmacokinetic Study Kim, Jin Hyeok Gao, Dan Cho, Chong Woon Hwang, Inkyu Kim, Hyung Min Kang, Jong Seong Plants (Basel) Article In this study, we developed a bioanalytical method using liquid chromatography coupled to triple quadrupole tandem mass spectrometry (LC-MS/MS) to apply to a pharmacokinetic study of inotodiol, which is known for its anti-cancer activity. Plasma samples were prepared with alkaline hydrolysis, liquid–liquid extraction, and solid-phase extraction. Inotodiol was detected in positive mode with atmospheric pressure chemical ionization by multiple-reaction monitoring mode using LC-MS/MS. The developed method was validated with linearity, accuracy, and precision. Accuracy ranged from 97.8% to 111.9%, and the coefficient of variation for precision was 1.8% to 4.4%. The developed method was applied for pharmacokinetic study, and the mean pharmacokinetic parameters administration were calculated as follows: λ(z) 0.016 min(−1); T(1/2) 49.35 min; C(max) 2582 ng/mL; Cl 0.004 ng/min; AUC(0–t) 109,500 ng×min/mL; MRT(0–t) 32.30 min; Vd 0.281 mL after intravenous administration at dose of 2 mg/kg and λ(z) 0.005 min(−1); T(1/2) 138.6 min; T(max) 40 min; C(max) 49.56 ng/mL; AUC(0–t) 6176 ng×in/mL; MRT(0–t) 103.7 min after oral administration. The absolute oral bioavailability of inotodiol was 0.45%, similar to nonpolar phytosterols. Collectively, this is the first bioanalytical method and pharmacokinetic study for inotodiol. MDPI 2021-08-09 /pmc/articles/PMC8401913/ /pubmed/34451676 http://dx.doi.org/10.3390/plants10081631 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Jin Hyeok Gao, Dan Cho, Chong Woon Hwang, Inkyu Kim, Hyung Min Kang, Jong Seong A Novel Bioanalytical Method for Determination of Inotodiol Isolated from Inonotus Obliquus and Its Application to Pharmacokinetic Study |
title | A Novel Bioanalytical Method for Determination of Inotodiol Isolated from Inonotus Obliquus and Its Application to Pharmacokinetic Study |
title_full | A Novel Bioanalytical Method for Determination of Inotodiol Isolated from Inonotus Obliquus and Its Application to Pharmacokinetic Study |
title_fullStr | A Novel Bioanalytical Method for Determination of Inotodiol Isolated from Inonotus Obliquus and Its Application to Pharmacokinetic Study |
title_full_unstemmed | A Novel Bioanalytical Method for Determination of Inotodiol Isolated from Inonotus Obliquus and Its Application to Pharmacokinetic Study |
title_short | A Novel Bioanalytical Method for Determination of Inotodiol Isolated from Inonotus Obliquus and Its Application to Pharmacokinetic Study |
title_sort | novel bioanalytical method for determination of inotodiol isolated from inonotus obliquus and its application to pharmacokinetic study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401913/ https://www.ncbi.nlm.nih.gov/pubmed/34451676 http://dx.doi.org/10.3390/plants10081631 |
work_keys_str_mv | AT kimjinhyeok anovelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy AT gaodan anovelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy AT chochongwoon anovelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy AT hwanginkyu anovelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy AT kimhyungmin anovelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy AT kangjongseong anovelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy AT kimjinhyeok novelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy AT gaodan novelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy AT chochongwoon novelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy AT hwanginkyu novelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy AT kimhyungmin novelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy AT kangjongseong novelbioanalyticalmethodfordeterminationofinotodiolisolatedfrominonotusobliquusanditsapplicationtopharmacokineticstudy |