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A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini

White lupin (Lupinus albus) represents an important legume crop in Europe and other parts of the world due to its high protein content and potential for low-input agriculture. However, most cultivars are susceptible to anthracnose caused by Colletotrichum lupini, a seed- and air-borne fungal pathoge...

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Autores principales: Kamber, Tim, Malpica-López, Nachelli, Messmer, Monika M., Oberhänsli, Thomas, Arncken, Christine, Alkemade, Joris A., Hohmann, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401954/
https://www.ncbi.nlm.nih.gov/pubmed/34451593
http://dx.doi.org/10.3390/plants10081548
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author Kamber, Tim
Malpica-López, Nachelli
Messmer, Monika M.
Oberhänsli, Thomas
Arncken, Christine
Alkemade, Joris A.
Hohmann, Pierre
author_facet Kamber, Tim
Malpica-López, Nachelli
Messmer, Monika M.
Oberhänsli, Thomas
Arncken, Christine
Alkemade, Joris A.
Hohmann, Pierre
author_sort Kamber, Tim
collection PubMed
description White lupin (Lupinus albus) represents an important legume crop in Europe and other parts of the world due to its high protein content and potential for low-input agriculture. However, most cultivars are susceptible to anthracnose caused by Colletotrichum lupini, a seed- and air-borne fungal pathogen that causes severe yield losses. The aim of this work was to develop a C. lupini-specific quantitative real-time TaqMan PCR assay that allows for quick and reliable detection and quantification of the pathogen in infected seed and plant material. Quantification of C. lupini DNA in dry seeds allowed us to distinguish infected and certified (non-infected) seed batches with DNA loads corresponding to the disease score index and yield of the mother plants. Additionally, C. lupini DNA could be detected in infected lupin shoots and close to the infection site, thereby allowing us to study the disease cycle of this hemibiotrophic pathogen. This qPCR assay provides a useful diagnostic tool to determine anthracnose infection levels of white lupin seeds and will facilitate the use of seed health assessments as a strategy to reduce the primary infection source and spread of this disease.
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spelling pubmed-84019542021-08-29 A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini Kamber, Tim Malpica-López, Nachelli Messmer, Monika M. Oberhänsli, Thomas Arncken, Christine Alkemade, Joris A. Hohmann, Pierre Plants (Basel) Article White lupin (Lupinus albus) represents an important legume crop in Europe and other parts of the world due to its high protein content and potential for low-input agriculture. However, most cultivars are susceptible to anthracnose caused by Colletotrichum lupini, a seed- and air-borne fungal pathogen that causes severe yield losses. The aim of this work was to develop a C. lupini-specific quantitative real-time TaqMan PCR assay that allows for quick and reliable detection and quantification of the pathogen in infected seed and plant material. Quantification of C. lupini DNA in dry seeds allowed us to distinguish infected and certified (non-infected) seed batches with DNA loads corresponding to the disease score index and yield of the mother plants. Additionally, C. lupini DNA could be detected in infected lupin shoots and close to the infection site, thereby allowing us to study the disease cycle of this hemibiotrophic pathogen. This qPCR assay provides a useful diagnostic tool to determine anthracnose infection levels of white lupin seeds and will facilitate the use of seed health assessments as a strategy to reduce the primary infection source and spread of this disease. MDPI 2021-07-28 /pmc/articles/PMC8401954/ /pubmed/34451593 http://dx.doi.org/10.3390/plants10081548 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kamber, Tim
Malpica-López, Nachelli
Messmer, Monika M.
Oberhänsli, Thomas
Arncken, Christine
Alkemade, Joris A.
Hohmann, Pierre
A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini
title A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini
title_full A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini
title_fullStr A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini
title_full_unstemmed A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini
title_short A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini
title_sort qpcr assay for the fast detection and quantification of colletotrichum lupini
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401954/
https://www.ncbi.nlm.nih.gov/pubmed/34451593
http://dx.doi.org/10.3390/plants10081548
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