A Comprehensive Study of the Antibacterial Activity of Bioactive Juice and Extracts from Pomegranate (Punica granatum L.) Peels and Seeds

Due to the growing awareness of Punica granatum fruit’s health-promoting properties, the pomegranate is increasingly used for food purposes. This results in the formation of biological waste products such as peels. A biowaste circular bioeconomy strategy holds great prospective for a sustainable eco...

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Autores principales: Kupnik, Kaja, Primožič, Mateja, Vasić, Katja, Knez, Željko, Leitgeb, Maja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402121/
https://www.ncbi.nlm.nih.gov/pubmed/34451599
http://dx.doi.org/10.3390/plants10081554
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author Kupnik, Kaja
Primožič, Mateja
Vasić, Katja
Knez, Željko
Leitgeb, Maja
author_facet Kupnik, Kaja
Primožič, Mateja
Vasić, Katja
Knez, Željko
Leitgeb, Maja
author_sort Kupnik, Kaja
collection PubMed
description Due to the growing awareness of Punica granatum fruit’s health-promoting properties, the pomegranate is increasingly used for food purposes. This results in the formation of biological waste products such as peels. A biowaste circular bioeconomy strategy holds great prospective for a sustainable economy. Therefore, a sustainable and environmentally friendly way of disposing of waste (e.g., use of biowaste to obtain high-value components (e.g., punicalagins, enzymes)) is crucial for the protection of the environment and human health. In the presented study, the content of total phenols and proanthocyanidins in ten samples of Punica granatum fruit (juice, aqueous (H(2)O) and ethanolic (EtOH) extracts of peels and seeds) was determined. Peel extracts were found to be the richest in the content of secondary metabolites and showed extremely high antioxidant potential (approximately 90% inhibition: DPPH radical scavenging activity). To the best of our knowledge, this is the first comparative study to determine the enzymatic activity of α-amylase, lipase, peroxidase, protease, and transglutaminase in different P. granatum samples. Furthermore, the antibacterial efficacy of all P. granatum samples was qualitatively determined against three strains of Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Pseudomonas fluorescens) and three strains of Gram-positive (Bacillus cereus, Staphylococcus aureus, and Streptococcus pyogenes) bacteria, susceptible to gaining antibiotic resistance. Moreover, the most promising peel extracts were quantified for antibacterial efficacy against tested bacteria at five different concentrations. All samples slowed down and inhibited the growth of all tested bacteria. MIC(90) values (2.7 or 0.3 mg/mL) were determined in 18 out of 24 experiments (four samples, six bacteria tested). There is no research in the reviewed literature that is current with such detailed and comprehensive determination of P. granatum peel extracts antibacterial activity. The results of the research showed great potential for the use of P. granatum in the field of antibacterial activity in biomedicine applications and in the cosmetic, food, and pharmaceutical industries.
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spelling pubmed-84021212021-08-29 A Comprehensive Study of the Antibacterial Activity of Bioactive Juice and Extracts from Pomegranate (Punica granatum L.) Peels and Seeds Kupnik, Kaja Primožič, Mateja Vasić, Katja Knez, Željko Leitgeb, Maja Plants (Basel) Article Due to the growing awareness of Punica granatum fruit’s health-promoting properties, the pomegranate is increasingly used for food purposes. This results in the formation of biological waste products such as peels. A biowaste circular bioeconomy strategy holds great prospective for a sustainable economy. Therefore, a sustainable and environmentally friendly way of disposing of waste (e.g., use of biowaste to obtain high-value components (e.g., punicalagins, enzymes)) is crucial for the protection of the environment and human health. In the presented study, the content of total phenols and proanthocyanidins in ten samples of Punica granatum fruit (juice, aqueous (H(2)O) and ethanolic (EtOH) extracts of peels and seeds) was determined. Peel extracts were found to be the richest in the content of secondary metabolites and showed extremely high antioxidant potential (approximately 90% inhibition: DPPH radical scavenging activity). To the best of our knowledge, this is the first comparative study to determine the enzymatic activity of α-amylase, lipase, peroxidase, protease, and transglutaminase in different P. granatum samples. Furthermore, the antibacterial efficacy of all P. granatum samples was qualitatively determined against three strains of Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Pseudomonas fluorescens) and three strains of Gram-positive (Bacillus cereus, Staphylococcus aureus, and Streptococcus pyogenes) bacteria, susceptible to gaining antibiotic resistance. Moreover, the most promising peel extracts were quantified for antibacterial efficacy against tested bacteria at five different concentrations. All samples slowed down and inhibited the growth of all tested bacteria. MIC(90) values (2.7 or 0.3 mg/mL) were determined in 18 out of 24 experiments (four samples, six bacteria tested). There is no research in the reviewed literature that is current with such detailed and comprehensive determination of P. granatum peel extracts antibacterial activity. The results of the research showed great potential for the use of P. granatum in the field of antibacterial activity in biomedicine applications and in the cosmetic, food, and pharmaceutical industries. MDPI 2021-07-28 /pmc/articles/PMC8402121/ /pubmed/34451599 http://dx.doi.org/10.3390/plants10081554 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kupnik, Kaja
Primožič, Mateja
Vasić, Katja
Knez, Željko
Leitgeb, Maja
A Comprehensive Study of the Antibacterial Activity of Bioactive Juice and Extracts from Pomegranate (Punica granatum L.) Peels and Seeds
title A Comprehensive Study of the Antibacterial Activity of Bioactive Juice and Extracts from Pomegranate (Punica granatum L.) Peels and Seeds
title_full A Comprehensive Study of the Antibacterial Activity of Bioactive Juice and Extracts from Pomegranate (Punica granatum L.) Peels and Seeds
title_fullStr A Comprehensive Study of the Antibacterial Activity of Bioactive Juice and Extracts from Pomegranate (Punica granatum L.) Peels and Seeds
title_full_unstemmed A Comprehensive Study of the Antibacterial Activity of Bioactive Juice and Extracts from Pomegranate (Punica granatum L.) Peels and Seeds
title_short A Comprehensive Study of the Antibacterial Activity of Bioactive Juice and Extracts from Pomegranate (Punica granatum L.) Peels and Seeds
title_sort comprehensive study of the antibacterial activity of bioactive juice and extracts from pomegranate (punica granatum l.) peels and seeds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402121/
https://www.ncbi.nlm.nih.gov/pubmed/34451599
http://dx.doi.org/10.3390/plants10081554
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