Cargando…

Cellular NADH and NADPH Conformation as a Real-Time Fluorescence-Based Metabolic Indicator under Pressurized Conditions

Cellular conformation of reduced pyridine nucleotides NADH and NADPH sensed using autofluorescence spectroscopy is presented as a real-time metabolic indicator under pressurized conditions. The approach provides information on the role of pressure in energy metabolism and antioxidant defense with ap...

Descripción completa

Detalles Bibliográficos
Autores principales:	Heidelman, Martin, Dhakal, Bibek, Gikunda, Millicent, Silva, Kalinga Pavan Thushara, Risal, Laxmi, Rodriguez, Andrew I., Abe, Fumiyoshi, Urayama, Paul
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402201/ https://www.ncbi.nlm.nih.gov/pubmed/34443607 http://dx.doi.org/10.3390/molecules26165020

Ejemplares similares

Separating NADH and NADPH fluorescence in live cells and tissues using FLIM
por: Blacker, Thomas S., et al.
Publicado: (2014)

In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD(+) fluorescent protein sensors
por: Lim, Shey-Li, et al.
Publicado: (2020)

Distinguishing chemically induced NADPH- and NADH-related metabolic responses using phasor analysis of UV-excited autofluorescence
por: Short, Audrey H., et al.
Publicado: (2021)

Investigating mitochondrial redox state using NADH and NADPH autofluorescence
por: Blacker, Thomas S., et al.
Publicado: (2016)

Quantification of the hydride donor abilities of NADH, NADPH, and BH(3)CN(−) in water
por: Mayer, Robert J., et al.
Publicado: (2022)

Upregulation of NADH/NADPH oxidase 4 by angiotensin II induces podocyte apoptosis
por: Ha, Tae-Sun, et al.
Publicado: (2023)

NADH and NADPH peroxidases as antioxidant defense mechanisms in intestinal sulfate-reducing bacteria
por: Kushkevych, Ivan, et al.
Publicado: (2023)

Metabolic trajectory of cellular differentiation in small intestine by Phasor Fluorescence Lifetime Microscopy of NADH
por: Stringari, Chiara, et al.
Publicado: (2012)

Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors
por: Meng, Hengkai, et al.
Publicado: (2016)

NADH/NADPH bi-cofactor-utilizing and thermoactive ketol-acid reductoisomerase from Sulfolobus acidocaldarius
por: Chen, Chin-Yu, et al.
Publicado: (2018)

A Novel Strategy Involved Anti-Oxidative Defense: The Conversion of NADH into NADPH by a Metabolic Network
por: Singh, Ranji, et al.
Publicado: (2008)

Correction: A Novel Strategy Involved in Anti-Oxidative Defense: The Conversion of NADH into NADPH by a Metabolic Network
por: Singh, Ranji, et al.
Publicado: (2008)

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts
por: Asfour, Huda, et al.
Publicado: (2012)

Mechanisms of Spontaneous Curvature Inversion in Compressed Graphene Ripples for Energy Harvesting Applications via Molecular Dynamics Simulations
por: Mangum, James M., et al.
Publicado: (2021)

Changes of NADH Fluorescence from the Skin of Patients with Systemic Lupus Erythematosus
por: Bogaczewicz, Jaroslaw, et al.
Publicado: (2019)

Redox engineering by ectopic expression of glutamate dehydrogenase genes links NADPH availability and NADH oxidation with cold growth in Saccharomyces cerevisiae
por: Ballester-Tomás, Lidia, et al.
Publicado: (2015)

Conformational coupling of redox-driven Na(+)-translocation in Vibrio cholerae NADH:quinone oxidoreductase
por: Hau, Jann-Louis, et al.
Publicado: (2023)

Using Fractional Intensities of Time-resolved Fluorescence to Sensitively Quantify NADH/NAD(+) with Genetically Encoded Fluorescent Biosensors
por: Chang, Mengfang, et al.
Publicado: (2017)

Nonequivalence of Second Sphere “Noncatalytic” Residues in Pentaerythritol Tetranitrate Reductase in Relation to Local Dynamics Linked to H-Transfer in Reactions with NADH and NADPH Coenzymes
por: Iorgu, Andreea I., et al.
Publicado: (2018)

In vivo imaging of cerebral energy metabolism with two-photon fluorescence lifetime microscopy of NADH
por: Yaseen, Mohammad A., et al.
Publicado: (2013)

Synthetic lethality of Mycobacterium tuberculosis NADH dehydrogenases is due to impaired NADH oxidation
por: Xu, Yuanyuan, et al.
Publicado: (2023)

Conformational States and Kinetics of the Calcium Binding Domain of NADPH Oxidase 5
por: Wei, Chin-Chuan, et al.
Publicado: (2010)

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence
por: Polesskaya, Oksana, et al.
Publicado: (2012)

Two-Photon Excited Fluorescence of NADH-Alcohol Dehydrogenase Complex in a Mixture with Bacterial Enzymes
por: Gorbunova, Ioanna A., et al.
Publicado: (2023)

NADPH- and NADH-dependent metabolism of and DNA adduct formation by benzo[a]pyrene catalyzed with rat hepatic microsomes and cytochrome P450 1A1
por: Stiborová, Marie, et al.
Publicado: (2016)

Syntrophomonas wolfei Uses an NADH-Dependent, Ferredoxin-Independent [FeFe]-Hydrogenase To Reoxidize NADH
por: Losey, Nathaniel A., et al.
Publicado: (2017)

Revealing the Membrane-Bound Catalytic Oxidation of NADH by the Drug Target Type-II NADH Dehydrogenase
por: Godoy-Hernandez, Albert, et al.
Publicado: (2019)

Tracking Functional Tumor Cell Subpopulations of Malignant Glioma by Phasor Fluorescence Lifetime Imaging Microscopy of NADH
por: Trinh, Andrew L., et al.
Publicado: (2017)

Enzyme-dependent fluorescence recovery of NADH after photobleaching to assess dehydrogenase activity of isolated perfused hearts
por: Moreno, Angel, et al.
Publicado: (2017)

Bioenergetic Alterations of Metabolic Redox Coenzymes as NADH, FAD and FMN by Means of Fluorescence Lifetime Imaging Techniques
por: Kalinina, Sviatlana, et al.
Publicado: (2021)

Two-photon fluorescence lifetime imaging microscopy of NADH metabolism in HIV-1 infected cells and tissues
por: Snyder, Greg A., et al.
Publicado: (2023)

Conformational Equilibrium of NADPH–Cytochrome P450 Oxidoreductase Is Essential for Heme Oxygenase Reaction
por: Sugishima, Masakazu, et al.
Publicado: (2020)

Single-molecule fluorescence-based analysis of protein conformation, interaction, and oligomerization in cellular systems
por: Okamoto, Kenji, et al.
Publicado: (2017)

A Photoenzymatic NADH Regeneration System
por: Höfler, Georg T., et al.
Publicado: (2018)

Mycoplasma bovis NADH oxidase functions as both a NADH oxidizing and O(2) reducing enzyme and an adhesin
por: Zhao, Gang, et al.
Publicado: (2017)

A neural network model predicts community-level signaling states in a diverse microbial community
por: Silva, Kalinga Pavan T., et al.
Publicado: (2019)

Phasor Fluorescence Lifetime Microscopy of Free and Protein-Bound NADH Reveals Neural Stem Cell Differentiation Potential
por: Stringari, Chiara, et al.
Publicado: (2012)

Geomorphodynamics, evolution, and ecology of vertical roots
por: Heidelman, Martin, et al.
Publicado: (2023)

Efflux pump gene amplifications bypass necessity of multiple target mutations for resistance against dual-targeting antibiotic
por: Silva, Kalinga Pavan T., et al.
Publicado: (2023)

Synthesis of [4S‐(2)H]NADH, [4R‐(2)H]NADH, [4‐(2)H(2)]NADH and [4‐(2)H]NAD(+) cofactors through heterogeneous biocatalysis in heavy water
por: Rowbotham, Jack S., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_llp27kq2t74op0hutp7b6s3t47