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Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein

To date, SARS-CoV-2 pandemic has caused more than 188 million infections and 4.06 million deaths worldwide. The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein has been regarded as an important target for vaccine and therapeutics development because it plays a key role in binding the h...

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Autores principales: Hong, Junping, Wang, Qian, Wu, Qian, Chen, Junyu, Wang, Xijing, Wang, Yingbin, Chen, Yixin, Xia, Ningshao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402368/
https://www.ncbi.nlm.nih.gov/pubmed/34451954
http://dx.doi.org/10.3390/vaccines9080829
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author Hong, Junping
Wang, Qian
Wu, Qian
Chen, Junyu
Wang, Xijing
Wang, Yingbin
Chen, Yixin
Xia, Ningshao
author_facet Hong, Junping
Wang, Qian
Wu, Qian
Chen, Junyu
Wang, Xijing
Wang, Yingbin
Chen, Yixin
Xia, Ningshao
author_sort Hong, Junping
collection PubMed
description To date, SARS-CoV-2 pandemic has caused more than 188 million infections and 4.06 million deaths worldwide. The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein has been regarded as an important target for vaccine and therapeutics development because it plays a key role in binding the human cell receptor ACE2 that is required for viral entry. However, it is not easy to detect RBD in Western blot using polyclonal antibody, suggesting that RBD may form a complicated conformation under native condition and bear rare linear epitope. So far, no linear epitope on RBD is reported. Thus, a monoclonal antibody (mAb) that recognizes linear epitope on RBD will become valuable. In the present study, an RBD-specific rabbit antibody named 9E1 was isolated from peripheral blood mononuclear cells (PBMC) of immunized rabbit by RBD-specific single B cell sorting and mapped to a highly conserved linear epitope within twelve amino acids (480)CNGVEGFNCYFP(491) on RBD. 9E1 works well in Western blot on S protein and immunohistochemistry on the SARS-CoV-2 infected tissue sections. The results demonstrated that 9E1 can be used as a useful tool for pathological and functional studies of SARS-CoV-2.
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spelling pubmed-84023682021-08-29 Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein Hong, Junping Wang, Qian Wu, Qian Chen, Junyu Wang, Xijing Wang, Yingbin Chen, Yixin Xia, Ningshao Vaccines (Basel) Article To date, SARS-CoV-2 pandemic has caused more than 188 million infections and 4.06 million deaths worldwide. The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein has been regarded as an important target for vaccine and therapeutics development because it plays a key role in binding the human cell receptor ACE2 that is required for viral entry. However, it is not easy to detect RBD in Western blot using polyclonal antibody, suggesting that RBD may form a complicated conformation under native condition and bear rare linear epitope. So far, no linear epitope on RBD is reported. Thus, a monoclonal antibody (mAb) that recognizes linear epitope on RBD will become valuable. In the present study, an RBD-specific rabbit antibody named 9E1 was isolated from peripheral blood mononuclear cells (PBMC) of immunized rabbit by RBD-specific single B cell sorting and mapped to a highly conserved linear epitope within twelve amino acids (480)CNGVEGFNCYFP(491) on RBD. 9E1 works well in Western blot on S protein and immunohistochemistry on the SARS-CoV-2 infected tissue sections. The results demonstrated that 9E1 can be used as a useful tool for pathological and functional studies of SARS-CoV-2. MDPI 2021-07-28 /pmc/articles/PMC8402368/ /pubmed/34451954 http://dx.doi.org/10.3390/vaccines9080829 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hong, Junping
Wang, Qian
Wu, Qian
Chen, Junyu
Wang, Xijing
Wang, Yingbin
Chen, Yixin
Xia, Ningshao
Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein
title Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein
title_full Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein
title_fullStr Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein
title_full_unstemmed Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein
title_short Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein
title_sort rabbit monoclonal antibody specifically recognizing a linear epitope in the rbd of sars-cov-2 spike protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402368/
https://www.ncbi.nlm.nih.gov/pubmed/34451954
http://dx.doi.org/10.3390/vaccines9080829
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