Cholera Toxin as a Probe for Membrane Biology

Cholera toxin B-subunit (CTxB) has emerged as one of the most widely utilized tools in membrane biology and biophysics. CTxB is a homopentameric stable protein that binds tightly to up to five GM1 glycosphingolipids. This provides a robust and tractable model for exploring membrane structure and its...

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Autores principales: Kenworthy, Anne K., Schmieder, Stefanie S., Raghunathan, Krishnan, Tiwari, Ajit, Wang, Ting, Kelly, Christopher V., Lencer, Wayne I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402489/
https://www.ncbi.nlm.nih.gov/pubmed/34437414
http://dx.doi.org/10.3390/toxins13080543
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author Kenworthy, Anne K.
Schmieder, Stefanie S.
Raghunathan, Krishnan
Tiwari, Ajit
Wang, Ting
Kelly, Christopher V.
Lencer, Wayne I.
author_facet Kenworthy, Anne K.
Schmieder, Stefanie S.
Raghunathan, Krishnan
Tiwari, Ajit
Wang, Ting
Kelly, Christopher V.
Lencer, Wayne I.
author_sort Kenworthy, Anne K.
collection PubMed
description Cholera toxin B-subunit (CTxB) has emerged as one of the most widely utilized tools in membrane biology and biophysics. CTxB is a homopentameric stable protein that binds tightly to up to five GM1 glycosphingolipids. This provides a robust and tractable model for exploring membrane structure and its dynamics including vesicular trafficking and nanodomain assembly. Here, we review important advances in these fields enabled by use of CTxB and its lipid receptor GM1.
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spelling pubmed-84024892021-08-29 Cholera Toxin as a Probe for Membrane Biology Kenworthy, Anne K. Schmieder, Stefanie S. Raghunathan, Krishnan Tiwari, Ajit Wang, Ting Kelly, Christopher V. Lencer, Wayne I. Toxins (Basel) Review Cholera toxin B-subunit (CTxB) has emerged as one of the most widely utilized tools in membrane biology and biophysics. CTxB is a homopentameric stable protein that binds tightly to up to five GM1 glycosphingolipids. This provides a robust and tractable model for exploring membrane structure and its dynamics including vesicular trafficking and nanodomain assembly. Here, we review important advances in these fields enabled by use of CTxB and its lipid receptor GM1. MDPI 2021-08-03 /pmc/articles/PMC8402489/ /pubmed/34437414 http://dx.doi.org/10.3390/toxins13080543 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Kenworthy, Anne K.
Schmieder, Stefanie S.
Raghunathan, Krishnan
Tiwari, Ajit
Wang, Ting
Kelly, Christopher V.
Lencer, Wayne I.
Cholera Toxin as a Probe for Membrane Biology
title Cholera Toxin as a Probe for Membrane Biology
title_full Cholera Toxin as a Probe for Membrane Biology
title_fullStr Cholera Toxin as a Probe for Membrane Biology
title_full_unstemmed Cholera Toxin as a Probe for Membrane Biology
title_short Cholera Toxin as a Probe for Membrane Biology
title_sort cholera toxin as a probe for membrane biology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402489/
https://www.ncbi.nlm.nih.gov/pubmed/34437414
http://dx.doi.org/10.3390/toxins13080543
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