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Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays

Solid organ transplant recipients, due to the administration of post-transplant immunosuppressive therapies, are at greater risk of viral reactivation episodes, mainly from herpes viruses, including varicella-zoster virus (VZV). The aim of this pilot study was to develop functional immunological ass...

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Autores principales: Cassaniti, Irene, Ferrari, Alessandro, Comolli, Giuditta, Sarasini, Antonella, Gregorini, Marilena, Rampino, Teresa, Lilleri, Daniele, Baldanti, Fausto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402512/
https://www.ncbi.nlm.nih.gov/pubmed/34451999
http://dx.doi.org/10.3390/vaccines9080875
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author Cassaniti, Irene
Ferrari, Alessandro
Comolli, Giuditta
Sarasini, Antonella
Gregorini, Marilena
Rampino, Teresa
Lilleri, Daniele
Baldanti, Fausto
author_facet Cassaniti, Irene
Ferrari, Alessandro
Comolli, Giuditta
Sarasini, Antonella
Gregorini, Marilena
Rampino, Teresa
Lilleri, Daniele
Baldanti, Fausto
author_sort Cassaniti, Irene
collection PubMed
description Solid organ transplant recipients, due to the administration of post-transplant immunosuppressive therapies, are at greater risk of viral reactivation episodes, mainly from herpes viruses, including varicella-zoster virus (VZV). The aim of this pilot study was to develop functional immunological assays (VZV-ELISpot) for the quantification and characterization of the VZV-specific effector-memory and central-memory responses in healthy subjects and transplanted patients. Glycoprotein gE and immediate-early 63 (IE-63) were used as antigens for in vitro stimulation. VZV-seropositive healthy subjects showed higher responses in respect to seronegative subjects. Even if differences were observed between VZV-seropositive healthy subjects and transplanted subjects at pre-transplant, the VZV-specific T-cell response was reduced at 60 days after transplant, mainly for the high level of immunosuppression. Phenotypical characterization revealed that response against VZV was mainly mediated by CD4 T cells. The results obtained in this study might be useful for the definition of personalized follow-up of the transplanted patients, providing useful information on the status of the patient potentially at risk of viral reactivation or other opportunistic infections.
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spelling pubmed-84025122021-08-29 Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays Cassaniti, Irene Ferrari, Alessandro Comolli, Giuditta Sarasini, Antonella Gregorini, Marilena Rampino, Teresa Lilleri, Daniele Baldanti, Fausto Vaccines (Basel) Article Solid organ transplant recipients, due to the administration of post-transplant immunosuppressive therapies, are at greater risk of viral reactivation episodes, mainly from herpes viruses, including varicella-zoster virus (VZV). The aim of this pilot study was to develop functional immunological assays (VZV-ELISpot) for the quantification and characterization of the VZV-specific effector-memory and central-memory responses in healthy subjects and transplanted patients. Glycoprotein gE and immediate-early 63 (IE-63) were used as antigens for in vitro stimulation. VZV-seropositive healthy subjects showed higher responses in respect to seronegative subjects. Even if differences were observed between VZV-seropositive healthy subjects and transplanted subjects at pre-transplant, the VZV-specific T-cell response was reduced at 60 days after transplant, mainly for the high level of immunosuppression. Phenotypical characterization revealed that response against VZV was mainly mediated by CD4 T cells. The results obtained in this study might be useful for the definition of personalized follow-up of the transplanted patients, providing useful information on the status of the patient potentially at risk of viral reactivation or other opportunistic infections. MDPI 2021-08-06 /pmc/articles/PMC8402512/ /pubmed/34451999 http://dx.doi.org/10.3390/vaccines9080875 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cassaniti, Irene
Ferrari, Alessandro
Comolli, Giuditta
Sarasini, Antonella
Gregorini, Marilena
Rampino, Teresa
Lilleri, Daniele
Baldanti, Fausto
Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays
title Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays
title_full Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays
title_fullStr Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays
title_full_unstemmed Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays
title_short Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays
title_sort characterization of varicella-zoster (vzv) specific t cell response in healthy subjects and transplanted patients by using enzyme linked immunospot (elispot) assays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402512/
https://www.ncbi.nlm.nih.gov/pubmed/34451999
http://dx.doi.org/10.3390/vaccines9080875
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