Cargando…
Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays
Solid organ transplant recipients, due to the administration of post-transplant immunosuppressive therapies, are at greater risk of viral reactivation episodes, mainly from herpes viruses, including varicella-zoster virus (VZV). The aim of this pilot study was to develop functional immunological ass...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402512/ https://www.ncbi.nlm.nih.gov/pubmed/34451999 http://dx.doi.org/10.3390/vaccines9080875 |
_version_ | 1783745807672934400 |
---|---|
author | Cassaniti, Irene Ferrari, Alessandro Comolli, Giuditta Sarasini, Antonella Gregorini, Marilena Rampino, Teresa Lilleri, Daniele Baldanti, Fausto |
author_facet | Cassaniti, Irene Ferrari, Alessandro Comolli, Giuditta Sarasini, Antonella Gregorini, Marilena Rampino, Teresa Lilleri, Daniele Baldanti, Fausto |
author_sort | Cassaniti, Irene |
collection | PubMed |
description | Solid organ transplant recipients, due to the administration of post-transplant immunosuppressive therapies, are at greater risk of viral reactivation episodes, mainly from herpes viruses, including varicella-zoster virus (VZV). The aim of this pilot study was to develop functional immunological assays (VZV-ELISpot) for the quantification and characterization of the VZV-specific effector-memory and central-memory responses in healthy subjects and transplanted patients. Glycoprotein gE and immediate-early 63 (IE-63) were used as antigens for in vitro stimulation. VZV-seropositive healthy subjects showed higher responses in respect to seronegative subjects. Even if differences were observed between VZV-seropositive healthy subjects and transplanted subjects at pre-transplant, the VZV-specific T-cell response was reduced at 60 days after transplant, mainly for the high level of immunosuppression. Phenotypical characterization revealed that response against VZV was mainly mediated by CD4 T cells. The results obtained in this study might be useful for the definition of personalized follow-up of the transplanted patients, providing useful information on the status of the patient potentially at risk of viral reactivation or other opportunistic infections. |
format | Online Article Text |
id | pubmed-8402512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84025122021-08-29 Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays Cassaniti, Irene Ferrari, Alessandro Comolli, Giuditta Sarasini, Antonella Gregorini, Marilena Rampino, Teresa Lilleri, Daniele Baldanti, Fausto Vaccines (Basel) Article Solid organ transplant recipients, due to the administration of post-transplant immunosuppressive therapies, are at greater risk of viral reactivation episodes, mainly from herpes viruses, including varicella-zoster virus (VZV). The aim of this pilot study was to develop functional immunological assays (VZV-ELISpot) for the quantification and characterization of the VZV-specific effector-memory and central-memory responses in healthy subjects and transplanted patients. Glycoprotein gE and immediate-early 63 (IE-63) were used as antigens for in vitro stimulation. VZV-seropositive healthy subjects showed higher responses in respect to seronegative subjects. Even if differences were observed between VZV-seropositive healthy subjects and transplanted subjects at pre-transplant, the VZV-specific T-cell response was reduced at 60 days after transplant, mainly for the high level of immunosuppression. Phenotypical characterization revealed that response against VZV was mainly mediated by CD4 T cells. The results obtained in this study might be useful for the definition of personalized follow-up of the transplanted patients, providing useful information on the status of the patient potentially at risk of viral reactivation or other opportunistic infections. MDPI 2021-08-06 /pmc/articles/PMC8402512/ /pubmed/34451999 http://dx.doi.org/10.3390/vaccines9080875 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cassaniti, Irene Ferrari, Alessandro Comolli, Giuditta Sarasini, Antonella Gregorini, Marilena Rampino, Teresa Lilleri, Daniele Baldanti, Fausto Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays |
title | Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays |
title_full | Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays |
title_fullStr | Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays |
title_full_unstemmed | Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays |
title_short | Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays |
title_sort | characterization of varicella-zoster (vzv) specific t cell response in healthy subjects and transplanted patients by using enzyme linked immunospot (elispot) assays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402512/ https://www.ncbi.nlm.nih.gov/pubmed/34451999 http://dx.doi.org/10.3390/vaccines9080875 |
work_keys_str_mv | AT cassanitiirene characterizationofvaricellazostervzvspecifictcellresponseinhealthysubjectsandtransplantedpatientsbyusingenzymelinkedimmunospotelispotassays AT ferrarialessandro characterizationofvaricellazostervzvspecifictcellresponseinhealthysubjectsandtransplantedpatientsbyusingenzymelinkedimmunospotelispotassays AT comolligiuditta characterizationofvaricellazostervzvspecifictcellresponseinhealthysubjectsandtransplantedpatientsbyusingenzymelinkedimmunospotelispotassays AT sarasiniantonella characterizationofvaricellazostervzvspecifictcellresponseinhealthysubjectsandtransplantedpatientsbyusingenzymelinkedimmunospotelispotassays AT gregorinimarilena characterizationofvaricellazostervzvspecifictcellresponseinhealthysubjectsandtransplantedpatientsbyusingenzymelinkedimmunospotelispotassays AT rampinoteresa characterizationofvaricellazostervzvspecifictcellresponseinhealthysubjectsandtransplantedpatientsbyusingenzymelinkedimmunospotelispotassays AT lilleridaniele characterizationofvaricellazostervzvspecifictcellresponseinhealthysubjectsandtransplantedpatientsbyusingenzymelinkedimmunospotelispotassays AT baldantifausto characterizationofvaricellazostervzvspecifictcellresponseinhealthysubjectsandtransplantedpatientsbyusingenzymelinkedimmunospotelispotassays |