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Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula

Discriminating Polistes dominula and Vespula spp. venom allergy is of growing importance worldwide, as systemic reactions to either species’ sting can lead to severe outcomes. Administering the correct allergen-specific immunotherapy is therefore a prerequisite to ensure the safety and health of ven...

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Autores principales: Grosch, Johannes, Eberlein, Bernadette, Waldherr, Sebastian, Pascal, Mariona, San Bartolomé, Clara, De La Roca Pinzón, Federico, Dittmar, Michael, Hilger, Christiane, Ollert, Markus, Biedermann, Tilo, Darsow, Ulf, Bilò, Maria Beatrice, Schmidt-Weber, Carsten B., Blank, Simon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402607/
https://www.ncbi.nlm.nih.gov/pubmed/34437431
http://dx.doi.org/10.3390/toxins13080559
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author Grosch, Johannes
Eberlein, Bernadette
Waldherr, Sebastian
Pascal, Mariona
San Bartolomé, Clara
De La Roca Pinzón, Federico
Dittmar, Michael
Hilger, Christiane
Ollert, Markus
Biedermann, Tilo
Darsow, Ulf
Bilò, Maria Beatrice
Schmidt-Weber, Carsten B.
Blank, Simon
author_facet Grosch, Johannes
Eberlein, Bernadette
Waldherr, Sebastian
Pascal, Mariona
San Bartolomé, Clara
De La Roca Pinzón, Federico
Dittmar, Michael
Hilger, Christiane
Ollert, Markus
Biedermann, Tilo
Darsow, Ulf
Bilò, Maria Beatrice
Schmidt-Weber, Carsten B.
Blank, Simon
author_sort Grosch, Johannes
collection PubMed
description Discriminating Polistes dominula and Vespula spp. venom allergy is of growing importance worldwide, as systemic reactions to either species’ sting can lead to severe outcomes. Administering the correct allergen-specific immunotherapy is therefore a prerequisite to ensure the safety and health of venom-allergic patients. Component-resolved diagnostics of Hymenoptera venom allergy might be improved by adding additional allergens to the diagnostic allergen panel. Therefore, three potential new allergens from P. dominula venom—immune responsive protein 30 (IRP30), vascular endothelial growth factor C (VEGF C) and phospholipase A2 (PLA2)—were cloned, recombinantly produced and biochemically characterized. Sera sIgE titers of Hymenoptera venom-allergic patients were measured in vitro to assess the allergenicity and potential cross-reactivity of the venom proteins. IRP30 and VEGF C were classified as minor allergens, as sensitization rates lay around 20–40%. About 50% of P. dominula venom-allergic patients had measurable sIgE titers directed against PLA2 from P. dominula venom. Interestingly, PLA2 was unable to activate basophils of allergic patients, questioning its role in the context of clinically relevant sensitization. Although the obtained results hint to a questionable benefit of the characterized P. dominula venom proteins for improved diagnosis of venom-allergic patients, they can contribute to a deeper understanding of the molecular mechanisms of Hymenoptera venoms and to the identification of factors that determine the allergenic potential of proteins.
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spelling pubmed-84026072021-08-29 Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula Grosch, Johannes Eberlein, Bernadette Waldherr, Sebastian Pascal, Mariona San Bartolomé, Clara De La Roca Pinzón, Federico Dittmar, Michael Hilger, Christiane Ollert, Markus Biedermann, Tilo Darsow, Ulf Bilò, Maria Beatrice Schmidt-Weber, Carsten B. Blank, Simon Toxins (Basel) Article Discriminating Polistes dominula and Vespula spp. venom allergy is of growing importance worldwide, as systemic reactions to either species’ sting can lead to severe outcomes. Administering the correct allergen-specific immunotherapy is therefore a prerequisite to ensure the safety and health of venom-allergic patients. Component-resolved diagnostics of Hymenoptera venom allergy might be improved by adding additional allergens to the diagnostic allergen panel. Therefore, three potential new allergens from P. dominula venom—immune responsive protein 30 (IRP30), vascular endothelial growth factor C (VEGF C) and phospholipase A2 (PLA2)—were cloned, recombinantly produced and biochemically characterized. Sera sIgE titers of Hymenoptera venom-allergic patients were measured in vitro to assess the allergenicity and potential cross-reactivity of the venom proteins. IRP30 and VEGF C were classified as minor allergens, as sensitization rates lay around 20–40%. About 50% of P. dominula venom-allergic patients had measurable sIgE titers directed against PLA2 from P. dominula venom. Interestingly, PLA2 was unable to activate basophils of allergic patients, questioning its role in the context of clinically relevant sensitization. Although the obtained results hint to a questionable benefit of the characterized P. dominula venom proteins for improved diagnosis of venom-allergic patients, they can contribute to a deeper understanding of the molecular mechanisms of Hymenoptera venoms and to the identification of factors that determine the allergenic potential of proteins. MDPI 2021-08-10 /pmc/articles/PMC8402607/ /pubmed/34437431 http://dx.doi.org/10.3390/toxins13080559 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grosch, Johannes
Eberlein, Bernadette
Waldherr, Sebastian
Pascal, Mariona
San Bartolomé, Clara
De La Roca Pinzón, Federico
Dittmar, Michael
Hilger, Christiane
Ollert, Markus
Biedermann, Tilo
Darsow, Ulf
Bilò, Maria Beatrice
Schmidt-Weber, Carsten B.
Blank, Simon
Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula
title Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula
title_full Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula
title_fullStr Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula
title_full_unstemmed Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula
title_short Characterization of New Allergens from the Venom of the European Paper Wasp Polistes dominula
title_sort characterization of new allergens from the venom of the european paper wasp polistes dominula
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402607/
https://www.ncbi.nlm.nih.gov/pubmed/34437431
http://dx.doi.org/10.3390/toxins13080559
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