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The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice

Wound healing is a physiological process comprising several coordinated phases, such as inflammation, proliferation, and remodeling. For centuries, Helix aspersa Muller mucus has been known to have biological properties that are useful for treating skin disorders. In this study, we used a full-thick...

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Autores principales: Gugliandolo, Enrico, Macrì, Francesco, Fusco, Roberta, Siracusa, Rosalba, D’Amico, Ramona, Cordaro, Marika, Peritore, Alessio Filippo, Impellizzeri, Daniela, Genovese, Tiziana, Cuzzocrea, Salvatore, Paola, Rosanna Di, Licata, Patrizia, Crupi, Rosalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402640/
https://www.ncbi.nlm.nih.gov/pubmed/34437489
http://dx.doi.org/10.3390/vetsci8080167
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author Gugliandolo, Enrico
Macrì, Francesco
Fusco, Roberta
Siracusa, Rosalba
D’Amico, Ramona
Cordaro, Marika
Peritore, Alessio Filippo
Impellizzeri, Daniela
Genovese, Tiziana
Cuzzocrea, Salvatore
Paola, Rosanna Di
Licata, Patrizia
Crupi, Rosalia
author_facet Gugliandolo, Enrico
Macrì, Francesco
Fusco, Roberta
Siracusa, Rosalba
D’Amico, Ramona
Cordaro, Marika
Peritore, Alessio Filippo
Impellizzeri, Daniela
Genovese, Tiziana
Cuzzocrea, Salvatore
Paola, Rosanna Di
Licata, Patrizia
Crupi, Rosalia
author_sort Gugliandolo, Enrico
collection PubMed
description Wound healing is a physiological process comprising several coordinated phases, such as inflammation, proliferation, and remodeling. For centuries, Helix aspersa Muller mucus has been known to have biological properties that are useful for treating skin disorders. In this study, we used a full-thickness excisional wound model in mice to test the hypothesis that Snail Secretion Filtrate (SSF) can improve the wound healing process. The mucus from Helix aspersa Muller was obtained mechanically by manually stimulating snails with a sterile cotton swab tip, and then the mucus was subjected to a series of filtrations to obtain SSF. After wounding, the mice were treated topically with SSF for 14 days. Our macroscopic results show that the SSF treatment significantly improved the speed and percentage of wound area closure. Furthermore, SSF improved several markers of proper wound healing, such as collagen deposition (Masson, COL3A1, matrix metalloproteinases (MMPs)) and the tissue remodeling process (α-sma, vascular-endothelial growth factor (VEGF)). SSF was also able to counteract the inflammatory process in injured wound tissue (myeloperoxidase (MPO) IL-1β, IL-6, TNF-α). In conclusion, our results show that SSF is able to enhance the speed and efficiency of wound healing and positively regulate several aspects of the wound healing process, such as the proliferative and remodeling phases.
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spelling pubmed-84026402021-08-29 The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice Gugliandolo, Enrico Macrì, Francesco Fusco, Roberta Siracusa, Rosalba D’Amico, Ramona Cordaro, Marika Peritore, Alessio Filippo Impellizzeri, Daniela Genovese, Tiziana Cuzzocrea, Salvatore Paola, Rosanna Di Licata, Patrizia Crupi, Rosalia Vet Sci Article Wound healing is a physiological process comprising several coordinated phases, such as inflammation, proliferation, and remodeling. For centuries, Helix aspersa Muller mucus has been known to have biological properties that are useful for treating skin disorders. In this study, we used a full-thickness excisional wound model in mice to test the hypothesis that Snail Secretion Filtrate (SSF) can improve the wound healing process. The mucus from Helix aspersa Muller was obtained mechanically by manually stimulating snails with a sterile cotton swab tip, and then the mucus was subjected to a series of filtrations to obtain SSF. After wounding, the mice were treated topically with SSF for 14 days. Our macroscopic results show that the SSF treatment significantly improved the speed and percentage of wound area closure. Furthermore, SSF improved several markers of proper wound healing, such as collagen deposition (Masson, COL3A1, matrix metalloproteinases (MMPs)) and the tissue remodeling process (α-sma, vascular-endothelial growth factor (VEGF)). SSF was also able to counteract the inflammatory process in injured wound tissue (myeloperoxidase (MPO) IL-1β, IL-6, TNF-α). In conclusion, our results show that SSF is able to enhance the speed and efficiency of wound healing and positively regulate several aspects of the wound healing process, such as the proliferative and remodeling phases. MDPI 2021-08-20 /pmc/articles/PMC8402640/ /pubmed/34437489 http://dx.doi.org/10.3390/vetsci8080167 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gugliandolo, Enrico
Macrì, Francesco
Fusco, Roberta
Siracusa, Rosalba
D’Amico, Ramona
Cordaro, Marika
Peritore, Alessio Filippo
Impellizzeri, Daniela
Genovese, Tiziana
Cuzzocrea, Salvatore
Paola, Rosanna Di
Licata, Patrizia
Crupi, Rosalia
The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice
title The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice
title_full The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice
title_fullStr The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice
title_full_unstemmed The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice
title_short The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice
title_sort protective effect of snail secretion filtrate in an experimental model of excisional wounds in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402640/
https://www.ncbi.nlm.nih.gov/pubmed/34437489
http://dx.doi.org/10.3390/vetsci8080167
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