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Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in Mice

The COVID-19 pandemic, caused by SARS-CoV-2, has rapidly spread to more than 222 countries and has put global public health at high risk. The world urgently needs cost-effective and safe SARS-CoV-2 vaccines, antiviral, and therapeutic drugs to control it. In this study, we engineered the receptor bi...

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Autores principales: Mamedov, Tarlan, Yuksel, Damla, Ilgın, Merve, Gurbuzaslan, Irem, Gulec, Burcu, Yetiskin, Hazel, Uygut, Muhammet Ali, Islam Pavel, Shaikh Terkis, Ozdarendeli, Aykut, Mammadova, Gulshan, Say, Deniz, Hasanova, Gulnara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402646/
https://www.ncbi.nlm.nih.gov/pubmed/34452461
http://dx.doi.org/10.3390/v13081595
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author Mamedov, Tarlan
Yuksel, Damla
Ilgın, Merve
Gurbuzaslan, Irem
Gulec, Burcu
Yetiskin, Hazel
Uygut, Muhammet Ali
Islam Pavel, Shaikh Terkis
Ozdarendeli, Aykut
Mammadova, Gulshan
Say, Deniz
Hasanova, Gulnara
author_facet Mamedov, Tarlan
Yuksel, Damla
Ilgın, Merve
Gurbuzaslan, Irem
Gulec, Burcu
Yetiskin, Hazel
Uygut, Muhammet Ali
Islam Pavel, Shaikh Terkis
Ozdarendeli, Aykut
Mammadova, Gulshan
Say, Deniz
Hasanova, Gulnara
author_sort Mamedov, Tarlan
collection PubMed
description The COVID-19 pandemic, caused by SARS-CoV-2, has rapidly spread to more than 222 countries and has put global public health at high risk. The world urgently needs cost-effective and safe SARS-CoV-2 vaccines, antiviral, and therapeutic drugs to control it. In this study, we engineered the receptor binding domain (RBD) of the SARS-CoV-2 spike (S) protein and produced it in the plant Nicotiana benthamiana in a glycosylated and deglycosylated form. Expression levels of both glycosylated (gRBD) and deglycosylated (dRBD) RBD were greater than 45 mg/kg fresh weight. The purification yields were 22 mg of pure protein/kg of plant biomass for gRBD and 20 mg for dRBD, which would be sufficient for commercialization of these vaccine candidates. The purified plant-produced RBD protein was recognized by an S protein-specific monoclonal antibody, demonstrating specific reactivity of the antibody to the plant-produced RBD proteins. The SARS-CoV-2 RBD showed specific binding to angiotensin converting enzyme 2 (ACE2), the SARS-CoV-2 receptor. In mice, the plant-produced RBD antigens elicited high titers of antibodies with a potent virus-neutralizing activity. To our knowledge, this is the first report demonstrating that mice immunized with plant-produced deglycosylated RBD form elicited high titer of RBD-specific antibodies with potent neutralizing activity against SARS-CoV-2 infection. Thus, obtained data support that plant-produced glycosylated and in vivo deglycosylated RBD antigens, developed in this study, are promising vaccine candidates for the prevention of COVID-19.
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spelling pubmed-84026462021-08-29 Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in Mice Mamedov, Tarlan Yuksel, Damla Ilgın, Merve Gurbuzaslan, Irem Gulec, Burcu Yetiskin, Hazel Uygut, Muhammet Ali Islam Pavel, Shaikh Terkis Ozdarendeli, Aykut Mammadova, Gulshan Say, Deniz Hasanova, Gulnara Viruses Article The COVID-19 pandemic, caused by SARS-CoV-2, has rapidly spread to more than 222 countries and has put global public health at high risk. The world urgently needs cost-effective and safe SARS-CoV-2 vaccines, antiviral, and therapeutic drugs to control it. In this study, we engineered the receptor binding domain (RBD) of the SARS-CoV-2 spike (S) protein and produced it in the plant Nicotiana benthamiana in a glycosylated and deglycosylated form. Expression levels of both glycosylated (gRBD) and deglycosylated (dRBD) RBD were greater than 45 mg/kg fresh weight. The purification yields were 22 mg of pure protein/kg of plant biomass for gRBD and 20 mg for dRBD, which would be sufficient for commercialization of these vaccine candidates. The purified plant-produced RBD protein was recognized by an S protein-specific monoclonal antibody, demonstrating specific reactivity of the antibody to the plant-produced RBD proteins. The SARS-CoV-2 RBD showed specific binding to angiotensin converting enzyme 2 (ACE2), the SARS-CoV-2 receptor. In mice, the plant-produced RBD antigens elicited high titers of antibodies with a potent virus-neutralizing activity. To our knowledge, this is the first report demonstrating that mice immunized with plant-produced deglycosylated RBD form elicited high titer of RBD-specific antibodies with potent neutralizing activity against SARS-CoV-2 infection. Thus, obtained data support that plant-produced glycosylated and in vivo deglycosylated RBD antigens, developed in this study, are promising vaccine candidates for the prevention of COVID-19. MDPI 2021-08-12 /pmc/articles/PMC8402646/ /pubmed/34452461 http://dx.doi.org/10.3390/v13081595 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mamedov, Tarlan
Yuksel, Damla
Ilgın, Merve
Gurbuzaslan, Irem
Gulec, Burcu
Yetiskin, Hazel
Uygut, Muhammet Ali
Islam Pavel, Shaikh Terkis
Ozdarendeli, Aykut
Mammadova, Gulshan
Say, Deniz
Hasanova, Gulnara
Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in Mice
title Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in Mice
title_full Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in Mice
title_fullStr Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in Mice
title_full_unstemmed Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in Mice
title_short Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in Mice
title_sort plant-produced glycosylated and in vivo deglycosylated receptor binding domain proteins of sars-cov-2 induce potent neutralizing responses in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402646/
https://www.ncbi.nlm.nih.gov/pubmed/34452461
http://dx.doi.org/10.3390/v13081595
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