Cargando…

The Requirement of Glycoprotein C for Interindividual Spread Is Functionally Conserved within the Alphaherpesvirus Genus (Mardivirus), but Not the Host (Gallid)

Marek’s disease (MD) in chickens is caused by Gallid alphaherpesvirus 2, better known as MD herpesvirus (MDV). Current vaccines do not block interindividual spread from chicken-to-chicken, therefore, understanding MDV interindividual spread provides important information for the development of poten...

Descripción completa

Detalles Bibliográficos
Autores principales: Vega-Rodriguez, Widaliz, Ponnuraj, Nagendraprabhu, Garcia, Maricarmen, Jarosinski, Keith W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402654/
https://www.ncbi.nlm.nih.gov/pubmed/34452285
http://dx.doi.org/10.3390/v13081419
_version_ 1783745842968002560
author Vega-Rodriguez, Widaliz
Ponnuraj, Nagendraprabhu
Garcia, Maricarmen
Jarosinski, Keith W.
author_facet Vega-Rodriguez, Widaliz
Ponnuraj, Nagendraprabhu
Garcia, Maricarmen
Jarosinski, Keith W.
author_sort Vega-Rodriguez, Widaliz
collection PubMed
description Marek’s disease (MD) in chickens is caused by Gallid alphaherpesvirus 2, better known as MD herpesvirus (MDV). Current vaccines do not block interindividual spread from chicken-to-chicken, therefore, understanding MDV interindividual spread provides important information for the development of potential therapies to protect against MD, while also providing a natural host to study herpesvirus dissemination. It has long been thought that glycoprotein C (gC) of alphaherpesviruses evolved with their host based on their ability to bind and inhibit complement in a species-selective manner. Here, we tested the functional importance of gC during interindividual spread and host specificity using the natural model system of MDV in chickens through classical compensation experiments. By exchanging MDV gC with another chicken alphaherpesvirus (Gallid alphaherpesvirus 1 or infectious laryngotracheitis virus; ILTV) gC, we determined that ILTV gC could not compensate for MDV gC during interindividual spread. In contrast, exchanging turkey herpesvirus (Meleagrid alphaherpesvirus 1 or HVT) gC could compensate for chicken MDV gC. Both ILTV and MDV are Gallid alphaherpesviruses; however, ILTV is a member of the Iltovirus genus, while MDV is classified as a Mardivirus along with HVT. These results suggest that gC is functionally conserved based on the virus genera (Mardivirus vs. Iltovirus) and not the host (Gallid vs. Meleagrid).
format Online
Article
Text
id pubmed-8402654
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84026542021-08-29 The Requirement of Glycoprotein C for Interindividual Spread Is Functionally Conserved within the Alphaherpesvirus Genus (Mardivirus), but Not the Host (Gallid) Vega-Rodriguez, Widaliz Ponnuraj, Nagendraprabhu Garcia, Maricarmen Jarosinski, Keith W. Viruses Article Marek’s disease (MD) in chickens is caused by Gallid alphaherpesvirus 2, better known as MD herpesvirus (MDV). Current vaccines do not block interindividual spread from chicken-to-chicken, therefore, understanding MDV interindividual spread provides important information for the development of potential therapies to protect against MD, while also providing a natural host to study herpesvirus dissemination. It has long been thought that glycoprotein C (gC) of alphaherpesviruses evolved with their host based on their ability to bind and inhibit complement in a species-selective manner. Here, we tested the functional importance of gC during interindividual spread and host specificity using the natural model system of MDV in chickens through classical compensation experiments. By exchanging MDV gC with another chicken alphaherpesvirus (Gallid alphaherpesvirus 1 or infectious laryngotracheitis virus; ILTV) gC, we determined that ILTV gC could not compensate for MDV gC during interindividual spread. In contrast, exchanging turkey herpesvirus (Meleagrid alphaherpesvirus 1 or HVT) gC could compensate for chicken MDV gC. Both ILTV and MDV are Gallid alphaherpesviruses; however, ILTV is a member of the Iltovirus genus, while MDV is classified as a Mardivirus along with HVT. These results suggest that gC is functionally conserved based on the virus genera (Mardivirus vs. Iltovirus) and not the host (Gallid vs. Meleagrid). MDPI 2021-07-21 /pmc/articles/PMC8402654/ /pubmed/34452285 http://dx.doi.org/10.3390/v13081419 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vega-Rodriguez, Widaliz
Ponnuraj, Nagendraprabhu
Garcia, Maricarmen
Jarosinski, Keith W.
The Requirement of Glycoprotein C for Interindividual Spread Is Functionally Conserved within the Alphaherpesvirus Genus (Mardivirus), but Not the Host (Gallid)
title The Requirement of Glycoprotein C for Interindividual Spread Is Functionally Conserved within the Alphaherpesvirus Genus (Mardivirus), but Not the Host (Gallid)
title_full The Requirement of Glycoprotein C for Interindividual Spread Is Functionally Conserved within the Alphaherpesvirus Genus (Mardivirus), but Not the Host (Gallid)
title_fullStr The Requirement of Glycoprotein C for Interindividual Spread Is Functionally Conserved within the Alphaherpesvirus Genus (Mardivirus), but Not the Host (Gallid)
title_full_unstemmed The Requirement of Glycoprotein C for Interindividual Spread Is Functionally Conserved within the Alphaherpesvirus Genus (Mardivirus), but Not the Host (Gallid)
title_short The Requirement of Glycoprotein C for Interindividual Spread Is Functionally Conserved within the Alphaherpesvirus Genus (Mardivirus), but Not the Host (Gallid)
title_sort requirement of glycoprotein c for interindividual spread is functionally conserved within the alphaherpesvirus genus (mardivirus), but not the host (gallid)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402654/
https://www.ncbi.nlm.nih.gov/pubmed/34452285
http://dx.doi.org/10.3390/v13081419
work_keys_str_mv AT vegarodriguezwidaliz therequirementofglycoproteincforinterindividualspreadisfunctionallyconservedwithinthealphaherpesvirusgenusmardivirusbutnotthehostgallid
AT ponnurajnagendraprabhu therequirementofglycoproteincforinterindividualspreadisfunctionallyconservedwithinthealphaherpesvirusgenusmardivirusbutnotthehostgallid
AT garciamaricarmen therequirementofglycoproteincforinterindividualspreadisfunctionallyconservedwithinthealphaherpesvirusgenusmardivirusbutnotthehostgallid
AT jarosinskikeithw therequirementofglycoproteincforinterindividualspreadisfunctionallyconservedwithinthealphaherpesvirusgenusmardivirusbutnotthehostgallid
AT vegarodriguezwidaliz requirementofglycoproteincforinterindividualspreadisfunctionallyconservedwithinthealphaherpesvirusgenusmardivirusbutnotthehostgallid
AT ponnurajnagendraprabhu requirementofglycoproteincforinterindividualspreadisfunctionallyconservedwithinthealphaherpesvirusgenusmardivirusbutnotthehostgallid
AT garciamaricarmen requirementofglycoproteincforinterindividualspreadisfunctionallyconservedwithinthealphaherpesvirusgenusmardivirusbutnotthehostgallid
AT jarosinskikeithw requirementofglycoproteincforinterindividualspreadisfunctionallyconservedwithinthealphaherpesvirusgenusmardivirusbutnotthehostgallid