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Clinical Course of Infection and Cross-Species Detection of Equine Parvovirus-Hepatitis

Since its first discovery by Arnold Theiler in 1918, serum hepatitis also known as Theiler’s disease has been reported worldwide, causing idiopathic acute hepatitis and liver failure in horses. Recent studies have suggested a novel parvovirus, named equine parvovirus hepatitis (EqPV-H), to be associ...

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Autores principales: Reinecke, Birthe, Klöhn, Mara, Brüggemann, Yannick, Kinast, Volker, Todt, Daniel, Stang, Alexander, Badenhorst, Marcha, Koeppel, Katja, Guthrie, Alan, Groner, Ursula, Puff, Christina, de le Roi, Madeleine, Baumgärtner, Wolfgang, Cavalleri, Jessika-M. V., Steinmann, Eike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402690/
https://www.ncbi.nlm.nih.gov/pubmed/34452320
http://dx.doi.org/10.3390/v13081454
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author Reinecke, Birthe
Klöhn, Mara
Brüggemann, Yannick
Kinast, Volker
Todt, Daniel
Stang, Alexander
Badenhorst, Marcha
Koeppel, Katja
Guthrie, Alan
Groner, Ursula
Puff, Christina
de le Roi, Madeleine
Baumgärtner, Wolfgang
Cavalleri, Jessika-M. V.
Steinmann, Eike
author_facet Reinecke, Birthe
Klöhn, Mara
Brüggemann, Yannick
Kinast, Volker
Todt, Daniel
Stang, Alexander
Badenhorst, Marcha
Koeppel, Katja
Guthrie, Alan
Groner, Ursula
Puff, Christina
de le Roi, Madeleine
Baumgärtner, Wolfgang
Cavalleri, Jessika-M. V.
Steinmann, Eike
author_sort Reinecke, Birthe
collection PubMed
description Since its first discovery by Arnold Theiler in 1918, serum hepatitis also known as Theiler’s disease has been reported worldwide, causing idiopathic acute hepatitis and liver failure in horses. Recent studies have suggested a novel parvovirus, named equine parvovirus hepatitis (EqPV-H), to be associated with Theiler’s disease. Despite the severity and potential fatality of EqPV-H infection, little is known about the possibility of developing chronic infections and putative cross-species infection of equine sister species. In the present longitudinal study, we employed qPCR analysis, serology, and biochemical testing as well as pathology examination of liver biopsies and sequence analysis to investigate potential chronic EqPV-H infection in an isolated study cohort of in total 124 horses from Germany over five years (2013–2018). Importantly, our data suggest that EqPV-H viremia can become chronic in infected horses that do not show biochemical and pathological signs of liver disease. Phylogenetic analysis by maximum likelihood model also confirms high sequence similarity and nucleotide conservation of the multidomain nuclear phosphoprotein NS1 sequences from equine serum samples collected between 2013–2018. Moreover, by examining human, zebra, and donkey sera for the presence of EqPV-H DNA and VP1 capsid protein antibodies, we found evidence for cross-species infection in donkey, but not to human and zebra. In conclusion, this study provides proof for the occurrence of persistent EqPV-H infection in asymptomatic horses and cross-species EqPV-H detection in donkeys.
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spelling pubmed-84026902021-08-29 Clinical Course of Infection and Cross-Species Detection of Equine Parvovirus-Hepatitis Reinecke, Birthe Klöhn, Mara Brüggemann, Yannick Kinast, Volker Todt, Daniel Stang, Alexander Badenhorst, Marcha Koeppel, Katja Guthrie, Alan Groner, Ursula Puff, Christina de le Roi, Madeleine Baumgärtner, Wolfgang Cavalleri, Jessika-M. V. Steinmann, Eike Viruses Article Since its first discovery by Arnold Theiler in 1918, serum hepatitis also known as Theiler’s disease has been reported worldwide, causing idiopathic acute hepatitis and liver failure in horses. Recent studies have suggested a novel parvovirus, named equine parvovirus hepatitis (EqPV-H), to be associated with Theiler’s disease. Despite the severity and potential fatality of EqPV-H infection, little is known about the possibility of developing chronic infections and putative cross-species infection of equine sister species. In the present longitudinal study, we employed qPCR analysis, serology, and biochemical testing as well as pathology examination of liver biopsies and sequence analysis to investigate potential chronic EqPV-H infection in an isolated study cohort of in total 124 horses from Germany over five years (2013–2018). Importantly, our data suggest that EqPV-H viremia can become chronic in infected horses that do not show biochemical and pathological signs of liver disease. Phylogenetic analysis by maximum likelihood model also confirms high sequence similarity and nucleotide conservation of the multidomain nuclear phosphoprotein NS1 sequences from equine serum samples collected between 2013–2018. Moreover, by examining human, zebra, and donkey sera for the presence of EqPV-H DNA and VP1 capsid protein antibodies, we found evidence for cross-species infection in donkey, but not to human and zebra. In conclusion, this study provides proof for the occurrence of persistent EqPV-H infection in asymptomatic horses and cross-species EqPV-H detection in donkeys. MDPI 2021-07-26 /pmc/articles/PMC8402690/ /pubmed/34452320 http://dx.doi.org/10.3390/v13081454 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reinecke, Birthe
Klöhn, Mara
Brüggemann, Yannick
Kinast, Volker
Todt, Daniel
Stang, Alexander
Badenhorst, Marcha
Koeppel, Katja
Guthrie, Alan
Groner, Ursula
Puff, Christina
de le Roi, Madeleine
Baumgärtner, Wolfgang
Cavalleri, Jessika-M. V.
Steinmann, Eike
Clinical Course of Infection and Cross-Species Detection of Equine Parvovirus-Hepatitis
title Clinical Course of Infection and Cross-Species Detection of Equine Parvovirus-Hepatitis
title_full Clinical Course of Infection and Cross-Species Detection of Equine Parvovirus-Hepatitis
title_fullStr Clinical Course of Infection and Cross-Species Detection of Equine Parvovirus-Hepatitis
title_full_unstemmed Clinical Course of Infection and Cross-Species Detection of Equine Parvovirus-Hepatitis
title_short Clinical Course of Infection and Cross-Species Detection of Equine Parvovirus-Hepatitis
title_sort clinical course of infection and cross-species detection of equine parvovirus-hepatitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402690/
https://www.ncbi.nlm.nih.gov/pubmed/34452320
http://dx.doi.org/10.3390/v13081454
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